LOCUS       40924_2144        9078 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector mTol2-B-P-Gal4VP16-Cre-CG3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9078)
  AUTHORS   Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, 
            Chien CB, Dorsky R.
  TITLE     Large Scale Zebrafish Gal4 Enhancer Trap Screen for Neural Specific 
            Expression Patterns
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9078)
  AUTHORS   Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, 
            Chien CB, Dorsky R.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAR-2013) Neurobiology and Anatomy, University of 
            Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA
REFERENCE   3  (bases 1 to 9078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-MAR-2013) Neurobiology and Anatomy, University of Utah, 20 North
            1900 East, Salt Lake City, UT 84132, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1..18)
                     /label=T7 primer
                     /note="T7 primer"
     primer_bind     complement(24..41)
                     /label=M13F
                     /note="M13F"
     primer_bind     complement(24..40)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    112..180
                     /label=LacZ alpha
                     /note="LacZ alpha"
     rep_origin      181..636
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        714..818
                     /label=AmpR promoter
     CDS             819..1676
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1850..2438
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2726..2747
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2762..2792
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2800..2816
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2824..2840
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2858..2876
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     repeat_region   2927..3085
                     /label=miniTol2 3' end
                     /note="miniTol2 3' end"
     repeat_region   2927..2943
                     /label=Tol2 3' TIR
                     /note="Tol2 3' TIR"
     primer_bind     3108..3129
                     /label=M13R
                     /note="M13R"
     primer_bind     3108..3124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3144..3164
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     regulatory      3193..3203
                     /label=adenovirus E1B TATA
                     /note="adenovirus E1B TATA"
                     /regulatory_class="promoter"
     5'UTR           3211..3242
                     /label=carp beta-actin trx start
                     /note="carp beta-actin trx start"
     protein_bind    3249..3282
                     /label=FRT (minimal)
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     protein_bind    3283..3307
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3313..3753
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     misc_feature    3790..3963
                     /label=VP16 activation domain
                     /note="VP16 activation domain"
     protein_bind    complement(3968..3992)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(3993..4010)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4015..4212
                     /label=SV40 late polyA (from pCS2+)(1)
                     /note="SV40 late polyA (from pCS2+)(1)"
     polyA_signal    4015..4149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(4261..4699)
                     /label=yeast ded1/HIS3 protein fragment X03245.1
                     /note="yeast ded1/HIS3 protein fragment X03245.1"
     intron          4706..4777
                     /note="SV40 small T antigen intron"
     intron          4782..4847
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4977..4997
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     polyA_signal    5422..5556
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_recomb     5598..5631
                     /label=FRT
                     /note="FRT"
     misc_feature    5598..5631
                     /label=FRT
                     /note="FRT"
     protein_bind    5598..5631
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     CDS             5651..5671
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5669..6697
                     /label=Cre
                     /note="site-specific recombinase"
     protein_bind    complement(6712..6732)
                     /label=attB3
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     primer_bind     complement(6740..6756)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(6772..6906)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    complement(7028..7160)
                     /label=SV40 late polyA from pCS2+(2)
                     /note="SV40 late polyA from pCS2+(2)"
     promoter        7165..7182
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(7211..7927)
                     /label=EGFP
                     /note="enhanced GFP"
     promoter        complement(7928..8826)
                     /label=cmlc2
                     /note="Cardiac myosin light chain 2 promoter"
     repeat_region   8850..9064
                     /label=miniTol2 5' end
                     /note="miniTol2 5' end"
     repeat_region   9046..9064
                     /label=Tol2 5' TIR
                     /note="Tol2 5' TIR"
     primer_bind     complement(9074..9078)
                     /label=T7 primer(1)
                     /note="T7 primer(1)"
ORIGIN
        1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
       61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
      241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac
      661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa
      721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
      781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
      841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
      901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
      961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
     2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
     2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac
     2881 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgagcaga ggtgtaaaaa
     2941 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta
     3001 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa
     3061 attgtacttg agtattaagg aagtagggat aacagggtaa tctcgagcag gaaacagcta
     3121 tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttgggccgg cccttcggcg
     3181 cgccactcta gagggtatat aatggatctc gcgtctcagc ctcactttga gctcctccac
     3241 accgcgccga agttcctatt ctctagaaag tataggaact tcccaagttt gtacaaaaaa
     3301 gcaggctgga ccatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt
     3361 aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg
     3421 gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa
     3481 gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac
     3541 cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta
     3601 tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact
     3661 gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt
     3721 agtaacaaag gtcaaagaca gttgactgta tcttcgaggt cgaccccggg aattcagatc
     3781 tctcgagccg cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag
     3841 gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac
     3901 ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat
     3961 atgtagcacc cagctttctt gtacaaagtg ggctatagtg agtcgtatta cgtagatcca
     4021 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa
     4081 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat
     4141 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg
     4201 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtacat taagggttcc
     4261 ggatcctcgg ggacaccaaa tatggcgatc tcggcctttt cgtttcttgg agctgggaca
     4321 tgtttgccat cgatccatct accaccagaa cggccgttag atctgctgcc accgttgttt
     4381 ccaccgaaga aaccaccgtt gccgtaacca ccacgacggt tgttgctaaa gaagctgcca
     4441 ccgccacggc caccgttgta gccgccgttg ttgttattgt agttgctcat gttatttctg
     4501 gcacttcttg gttttcctct taagtgagga ggaacataac cattctcgtt gttgtcgttg
     4561 atgcttaaat tttgcacttg ttcgctcagt tcagccataa tatgaaatgc ttttcttgtt
     4621 gttcttacgg aataccactt gccacctatc accacaacta actttttccc gttcctccat
     4681 ctcttttata ttttttttct cgagggatct ttgtgaagga accttacttc tgtggtgtga
     4741 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
     4801 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
     4861 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag
     4921 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa
     4981 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga
     5041 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa
     5101 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc
     5161 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg
     5221 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta
     5281 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt
     5341 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa
     5401 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc
     5461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     5521 tccaaactca tcaatgtatc ttatcatgtc tggatctgac atggtaagta agcttgggct
     5581 gcaggtcgag ggacctagaa gttcctattc tctagaaagt ataggaactt caagcttgtc
     5641 caccatggtg cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct
     5701 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt
     5761 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc
     5821 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt
     5881 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca
     5941 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa
     6001 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc
     6061 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa
     6121 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct
     6181 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg
     6241 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa
     6301 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc
     6361 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc
     6421 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct
     6481 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc
     6541 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca
     6601 ggctggtggc tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga
     6661 gactggggcc atggtgaggc tgctcgagga tggggactga aactgagtcg acaactttat
     6721 tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga tgatccagac
     6781 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc
     6841 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     6901 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag
     6961 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat
     7021 cagatctctt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7141 tatcttatca tgtctggatc tacgtaatac gactcactat agttctagag gctcgagagg
     7201 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     7261 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc
     7321 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     7381 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     7441 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     7501 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     7561 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     7621 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     7681 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     7741 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     7801 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     7861 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     7921 tcaccatggt cactgtctgc tttgctgttg gtctgggctc ctgggtcact ggcttactaa
     7981 tggagtcttt atgtatgagg actcttatca attgttcttc tataaaggtc tgcagtgttt
     8041 ctgttcgtcc cctacatgga cacccagagc ctcctaaata caggagccct gataactgca
     8101 caagtgctca gattccagca gggtggaaaa tgagataaag tgtgcagatg gggaggggga
     8161 cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt
     8221 cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga
     8281 tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat
     8341 tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg
     8401 aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa
     8461 gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga
     8521 aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag
     8581 tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta
     8641 tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa
     8701 ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc
     8761 tgatttggag agctcattta catttatttt gaatgtctct tatttaacac aactgattta
     8821 agctttagat ctattaccct gttatcccta gggaaaatag aatgaagtga tctccaaaaa
     8881 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa
     8941 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa
     9001 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc
     9061 tctgggccca attcgccc
//
