LOCUS 40924_2144 9078 bp DNA circular SYN 18-DEC-2018 DEFINITION Expression vector mTol2-B-P-Gal4VP16-Cre-CG3, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9078) AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R. TITLE Large Scale Zebrafish Gal4 Enhancer Trap Screen for Neural Specific Expression Patterns JOURNAL Unpublished REFERENCE 2 (bases 1 to 9078) AUTHORS Otsuna H, Scoresby AN, Gaynes B, Tong ZZ, Kwan KK, Hutcheson DA, Chien CB, Dorsky R. TITLE Direct Submission JOURNAL Submitted (19-MAR-2013) Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 9078) TITLE Direct Submission REFERENCE 4 (bases 1 to 9078) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (19-MAR-2013) Neurobiology and Anatomy, University of Utah, 20 North 1900 East, Salt Lake City, UT 84132, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..9078 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(1..18) /label=T7 primer /note="T7 primer" primer_bind complement(24..41) /label=M13F /note="M13F" primer_bind complement(24..40) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" misc_feature 112..180 /label=LacZ alpha /note="LacZ alpha" rep_origin 181..636 /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" promoter 714..818 /label=AmpR promoter CDS 819..1676 /label=AmpR /note="beta-lactamase" rep_origin 1850..2438 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 2726..2747 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 2762..2792 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2800..2816 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2824..2840 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" promoter 2858..2876 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" repeat_region 2927..3085 /label=miniTol2 3' end /note="miniTol2 3' end" repeat_region 2927..2943 /label=Tol2 3' TIR /note="Tol2 3' TIR" primer_bind 3108..3129 /label=M13R /note="M13R" primer_bind 3108..3124 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind 3144..3164 /label=attB4 /note="core recombination site for the Gateway(R) BP reaction" regulatory 3193..3203 /label=adenovirus E1B TATA /note="adenovirus E1B TATA" /regulatory_class="promoter" 5'UTR 3211..3242 /label=carp beta-actin trx start /note="carp beta-actin trx start" protein_bind 3249..3282 /label=FRT (minimal) /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" protein_bind 3283..3307 /label=attB1 /note="recombination site for the Gateway(R) BP reaction" CDS 3313..3753 /label=GAL4 DNA binding domain /note="DNA binding domain of the GAL4 transcriptional activator" misc_feature 3790..3963 /label=VP16 activation domain /note="VP16 activation domain" protein_bind complement(3968..3992) /label=attB2 /note="recombination site for the Gateway(R) BP reaction" promoter complement(3993..4010) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" misc_feature 4015..4212 /label=SV40 late polyA (from pCS2+)(1) /note="SV40 late polyA (from pCS2+)(1)" polyA_signal 4015..4149 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature complement(4261..4699) /label=yeast ded1/HIS3 protein fragment X03245.1 /note="yeast ded1/HIS3 protein fragment X03245.1" intron 4706..4777 /note="SV40 small T antigen intron" intron 4782..4847 /label=small t intron /note="SV40 (simian virus 40) small t antigen intron" CDS 4977..4997 /label=SV40 NLS /note="nuclear localization signal of SV40 (simian virus 40) large T antigen" polyA_signal 5422..5556 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_recomb 5598..5631 /label=FRT /note="FRT" misc_feature 5598..5631 /label=FRT /note="FRT" protein_bind 5598..5631 /label=FRT (minimal) /bound_moiety="FLP recombinase from the Saccharomyces cerevisiae 2u plasmid" /note="supports FLP-mediated excision but not integration (Turan and Bode, 2011)" CDS 5651..5671 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 5669..6697 /label=Cre /note="site-specific recombinase" protein_bind complement(6712..6732) /label=attB3 /note="core recombination site for the Gateway(R) BP reaction" primer_bind complement(6740..6756) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" polyA_signal complement(6772..6906) /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" misc_feature complement(7028..7160) /label=SV40 late polyA from pCS2+(2) /note="SV40 late polyA from pCS2+(2)" promoter 7165..7182 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS complement(7211..7927) /label=EGFP /note="enhanced GFP" promoter complement(7928..8826) /label=cmlc2 /note="Cardiac myosin light chain 2 promoter" repeat_region 8850..9064 /label=miniTol2 5' end /note="miniTol2 5' end" repeat_region 9046..9064 /label=Tol2 5' TIR /note="Tol2 5' TIR" primer_bind complement(9074..9078) /label=T7 primer(1) /note="T7 primer(1)" ORIGIN 1 tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 61 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 121 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 181 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 241 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 301 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 361 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 421 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 481 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 541 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 601 acgcgaattt taacaaaata ttaacgctta caatttcctg atgcggtatt ttctccttac 661 gcatctgtgc ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa 721 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 781 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 841 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 901 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 961 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 1021 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 1081 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 1141 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 1201 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 1261 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 1321 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 1381 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 1441 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 1501 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 1561 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 1621 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 1681 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 1741 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 1801 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 1861 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 1921 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 1981 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 2041 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 2101 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 2161 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 2221 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 2281 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 2341 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 2401 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 2461 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 2521 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 2581 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 2641 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga 2701 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg 2761 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc 2821 acacaggaaa cagctatgac catgattacg ccaagctatt taggtgacac tatagaatac 2881 tcaagctatg catccaacgc gttgggagct ctcccatatg gtcgagcaga ggtgtaaaaa 2941 gtactcaaaa attttactca agtgaaagta caagtactta gggaaaattt tactcaatta 3001 aaagtaaaag tatctggcta gaatcttact tgagtaaaag taaaaaagta ctccattaaa 3061 attgtacttg agtattaagg aagtagggat aacagggtaa tctcgagcag gaaacagcta 3121 tgaccatgat tacgccaagc tatcaacttt gtatagaaaa gttgggccgg cccttcggcg 3181 cgccactcta gagggtatat aatggatctc gcgtctcagc ctcactttga gctcctccac 3241 accgcgccga agttcctatt ctctagaaag tataggaact tcccaagttt gtacaaaaaa 3301 gcaggctgga ccatgaagct actgtcttct atcgaacaag catgcgatat ttgccgactt 3361 aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca agtgtctgaa gaacaactgg 3421 gagtgtcgct actctcccaa aaccaaaagg tctccgctga ctagggcaca tctgacagaa 3481 gtggaatcaa ggctagaaag actggaacag ctatttctac tgatttttcc tcgagaagac 3541 cttgacatga ttttgaaaat ggattcttta caggatataa aagcattgtt aacaggatta 3601 tttgtacaag ataatgtgaa taaagatgcc gtcacagata gattggcttc agtggagact 3661 gatatgcctc taacattgag acagcataga ataagtgcga catcatcatc ggaagagagt 3721 agtaacaaag gtcaaagaca gttgactgta tcttcgaggt cgaccccggg aattcagatc 3781 tctcgagccg cccccccgac cgatgtcagc ctgggggacg agctccactt agacggcgag 3841 gacgtggcga tggcgcatgc cgacgcgcta gacgatttcg atctggacat gttgggggac 3901 ggggattccc cgggtccggg atttaccccc cacgactccg ccccctacgg cgctctggat 3961 atgtagcacc cagctttctt gtacaaagtg ggctatagtg agtcgtatta cgtagatcca 4021 gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa 4081 tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat 4141 aaacaagtta acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg 4201 gaggtttttt aattcgcggc cgcggcgcca atgcattggg cccggtacat taagggttcc 4261 ggatcctcgg ggacaccaaa tatggcgatc tcggcctttt cgtttcttgg agctgggaca 4321 tgtttgccat cgatccatct accaccagaa cggccgttag atctgctgcc accgttgttt 4381 ccaccgaaga aaccaccgtt gccgtaacca ccacgacggt tgttgctaaa gaagctgcca 4441 ccgccacggc caccgttgta gccgccgttg ttgttattgt agttgctcat gttatttctg 4501 gcacttcttg gttttcctct taagtgagga ggaacataac cattctcgtt gttgtcgttg 4561 atgcttaaat tttgcacttg ttcgctcagt tcagccataa tatgaaatgc ttttcttgtt 4621 gttcttacgg aataccactt gccacctatc accacaacta actttttccc gttcctccat 4681 ctcttttata ttttttttct cgagggatct ttgtgaagga accttacttc tgtggtgtga 4741 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa 4801 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg 4861 gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg ttttgctcag 4921 aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct actcctccaa 4981 aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta agttttttga 5041 gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc acaaaggaaa 5101 aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt ataagtaggc 5161 ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat agagtgtctg 5221 ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt aaaggggtta 5281 ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca taccacattt 5341 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 5401 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 5461 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 5521 tccaaactca tcaatgtatc ttatcatgtc tggatctgac atggtaagta agcttgggct 5581 gcaggtcgag ggacctagaa gttcctattc tctagaaagt ataggaactt caagcttgtc 5641 caccatggtg cccaagaaga agaggaaagt ctccaacctg ctgactgtgc accaaaacct 5701 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt 5761 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc 5821 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt 5881 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca 5941 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa 6001 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc 6061 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa 6121 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct 6181 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg 6241 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa 6301 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc 6361 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc 6421 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct 6481 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc 6541 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca 6601 ggctggtggc tggaccaatg tgaacattgt gatgaactac atcagaaacc tggactctga 6661 gactggggcc atggtgaggc tgctcgagga tggggactga aactgagtcg acaactttat 6721 tatacatagt tgataattca ctggccgtcg ttttacggta ccatcgatga tgatccagac 6781 atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc 6841 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa 6901 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag 6961 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat 7021 cagatctctt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 7081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 7141 tatcttatca tgtctggatc tacgtaatac gactcactat agttctagag gctcgagagg 7201 ggccgcttta cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact 7261 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc 7321 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt 7381 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct 7441 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact 7501 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc 7561 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt 7621 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt 7681 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg 7741 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca 7801 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta 7861 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc 7921 tcaccatggt cactgtctgc tttgctgttg gtctgggctc ctgggtcact ggcttactaa 7981 tggagtcttt atgtatgagg actcttatca attgttcttc tataaaggtc tgcagtgttt 8041 ctgttcgtcc cctacatgga cacccagagc ctcctaaata caggagccct gataactgca 8101 caagtgctca gattccagca gggtggaaaa tgagataaag tgtgcagatg gggaggggga 8161 cgtgaatgag agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt 8221 cacaaaataa taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga 8281 tgtgatgttc atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat 8341 tttccttttc taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg 8401 aaaagattgg ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa 8461 gcaaacagca gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga 8521 aaccaacgac ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag 8581 tgcttgtgac tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta 8641 tcataaaaaa gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa 8701 ctggctatgc ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc 8761 tgatttggag agctcattta catttatttt gaatgtctct tatttaacac aactgattta 8821 agctttagat ctattaccct gttatcccta gggaaaatag aatgaagtga tctccaaaaa 8881 ataagtactt tttgactgta aataaaattg taaggagtaa aaagtacttt tttttctaaa 8941 aaaatgtaat taagtaaaag taaaagtatt gatttttaat tgtactcaag taaagtaaaa 9001 atccccaaaa ataatactta agtacagtaa tcaagtaaaa ttactcaagt actttacacc 9061 tctgggccca attcgccc //