Basic Vector Information
mceG vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
mceG vector Sequence
LOCUS 40924_1919 5871 bp DNA circular SYN 17-DEC-2018 DEFINITION Expression vector mceG, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5871) AUTHORS Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM. TITLE Characterization of mycobacterial virulence genes through genetic interaction mapping JOURNAL Proc. Natl. Acad. Sci. U.S.A. 103 (31), 11760-11765 (2006) PUBMED 16868085 REFERENCE 2 (bases 1 to 5871) AUTHORS Joshi SM, Pandey AK, Capite NM, Fortune SM, Rubin EJ, Sassetti CM. TITLE Direct Submission JOURNAL Submitted (26-JUN-2006) Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655, USA REFERENCE 3 (bases 1 to 5871) TITLE Direct Submission REFERENCE 4 (bases 1 to 5871) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl. Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "31"; pages: "11760-11765" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (26-JUN-2006) Molecular Genetics and Microbiology, University of Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 01655, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5871 /mol_type="other DNA" /organism="synthetic DNA construct" regulatory 7..394 /note="promoter from Mycobacterium tuberculosis groEL2 gene" /regulatory_class="promoter" CDS 443..1519 /gene="mkl" /label=mkl /note="Probable ribonucleotide transport ATP-binding protein mkl from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). Accession#: P9WQL5" CDS 1520..1537 /label=6xHis /note="6xHis affinity tag" CDS 1544..1573 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS 1931..2695 /label=ApmR /note="aminoglycoside 3-N-acetyltransferase type IV" rep_origin 3204..3792 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" rep_origin 3951..5845 /label=mycobacterial origin of replication from pAL5000 /note="mycobacterial origin of replication from pAL5000"
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