LOCUS       40924_1919        5871 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector mceG, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5871)
  AUTHORS   Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Characterization of mycobacterial virulence genes through genetic 
            interaction mapping
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (31), 11760-11765 (2006)
  PUBMED    16868085
REFERENCE   2  (bases 1 to 5871)
  AUTHORS   Joshi SM, Pandey AK, Capite NM, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2006) Molecular Genetics and Microbiology, 
            University of Massachusetts Medical School, 55 Lake Ave. North, 
            Worcester, MA 01655, USA
REFERENCE   3  (bases 1 to 5871)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5871)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "31"; pages:
            "11760-11765"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUN-2006) Molecular Genetics and Microbiology, University of 
            Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 
            01655, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5871
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..394
                     /note="promoter from Mycobacterium tuberculosis groEL2
                     gene"
                     /regulatory_class="promoter"
     CDS             443..1519
                     /gene="mkl"
                     /label=mkl
                     /note="Probable ribonucleotide transport ATP-binding
                     protein mkl from Mycobacterium tuberculosis (strain ATCC 
                     25618 / H37Rv). Accession#: P9WQL5"
     CDS             1520..1537
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1544..1573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1931..2695
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     rep_origin      3204..3792
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3951..5845
                     /label=mycobacterial origin of replication from pAL5000
                     /note="mycobacterial origin of replication from pAL5000"
ORIGIN
        1 tctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccatg gccaagacaa ttgcggatcc aactgggcgc
      421 cgaccacctg acggcgcctc gggtgcgata cagtgactca taccacacaa cgggccggtg
      481 gcagccacga gcgtcgacag aagggtttcc catgggcgtc agcatcgagg tcaacggact
      541 aacgaagtcc ttcgggtcct cgaggatctg ggaagatgtc acgctaacga tccccgccgg
      601 ggaggtcagc gtgctgctgg gcccatcggg taccggcaaa tcggtgtttc tgaaatctct
      661 gatcggcctc ctgcggccgg agcgcggctc gatcatcatc gacggcaccg acatcatcga
      721 atgctcggcc aaggagcttt acgagatccg cacattgttc ggcgtgctgt ttcaggacgg
      781 tgccctgttc gggtcgatga acctctacga caacaccgcg ttccccctgc gtgagcacac
      841 caagaaaaag gaaagcgaga tccgtgacat cgtcatggag aagctggccc tagtcggcct
      901 gggtggggac gagaagaagt tccccggcga gatctccggc gggatgcgta agcgtgccgg
      961 cctagcgcgt gccctggtcc ttgacccgca gatcattctc tgcgacgagc ccgactcggg
     1021 tctggacccg gttcgtaccg cctacctgag ccagctgatc atggacatca acgcccagat
     1081 cgacgccacc atcctgatcg tgacgcacaa catcaacatc gcccgcaccg tgccggacaa
     1141 catgggcatg ttgttccgca agcatttggt gatgttcggg ccgcgggagg tgctactcac
     1201 cagcgacgag ccggtggtgc ggcagttcct caacggccgg cgcatcggcc cgatcggcat
     1261 gtccgaggag aaggacgagg ccaccatggc cgaagagcag gccctgctcg atgccggcca
     1321 ccacgcgggc ggtgtcgagg aaatcgaggg cgtgccgccg cagatcagcg cgacaccggg
     1381 catgccggag cgcaaagcgg tcgcccggcg tcaggctcgg gttcgcgaga tgttgcacac
     1441 gctgcccaaa aaggcccagg cggcgatcct cgacgatctc gagggcacgc acaagtacgc
     1501 ggtgcacgaa atcggccagc accaccacca ccaccatatg gccgagcaga agctgatctc
     1561 ggaggaggac ctgtaaatac ccaggtcggc ggcttgaggg agccgcggtg acgacgtcga
     1621 caacgcttgg cggttacgtc cgcgaccaac tgcaaacccc gctgaccctc gtcggtggat
     1681 tctttcgcat gtgtgtgctg actggaaagg cgctgtttcg ctggccgttc cagtggcgcg
     1741 agttcattct gcagctcacg gtaactgatg ccgtatttgc agtaccagcg tacggcccac
     1801 agaatgatgt cacgctgaaa atgccggcct ttgaatgggt tcatgtgcag ctccatcagc
     1861 aaaaggggat gataagttta tcaccaccga ctatttgcaa cagtgccgtt gatcgtgcta
     1921 tgatcgactg atgtcatcag cggtggagtg caatgtcgtg caatacgaat ggcgaaaagc
     1981 cgagctcatc ggtcagcttc tcaaccttgg ggttaccccc ggcggtgtgc tgctggtcca
     2041 cagctccttc cgtagcgtcc ggcccctcga agatgggcca cttggactga tcgaggccct
     2101 gcgtgctgcg ctgggtccgg gagggacgct cgtcatgccc tcgtggtcag gtctggacga
     2161 cgagccgttc gatcctgcca cgtcgcccgt tacaccggac cttggagttg tctctgacac
     2221 attctggcgc ctgccaaatg taaagcgcag cgcccatcca tttgcctttg cggcagcggg
     2281 gccacaggca gagcagatca tctctgatcc attgcccctg ccacctcact cgcctgcaag
     2341 cccggtcgcc cgtgtccatg aactcgatgg gcaggtactt ctcctcggcg tgggacacga
     2401 tgccaacacg acgctgcatc ttgccgagtt gatggcaaag gttccctatg gggtgccgag
     2461 acactgcacc attcttcagg atggcaagtt ggtacgcgtc gattatctcg agaatgacca
     2521 ctgctgtgag cgctttgcct tggcggacag gtggctcaag gagaagagcc ttcagaagga
     2581 aggtccagtc ggtcatgcct ttgctcggtt gatccgctcc cgcgacattg tggcgacagc
     2641 cctgggtcaa ctgggccgag atccgttgat cttcctgcat ccgccagagg gcgggatgcg
     2701 aagaatgcga tgccgctcgc cagtcgattg gctgagctca tgagcggaga acgagatgac
     2761 gttggagggg caaggtcgcg ctgagaattc ctggtcgttc cgcaggctcg cgtaggaatc
     2821 atccgaatca atacggtcga gaagtaacag ggattcttgt gtcacagcgg acctctattc
     2881 acagggtacg ggccggctta attccgcacg gccggtcgcg acacggcctg tccgcaccgc
     2941 ggtcaggcgt tgacgatgac gggctggtcg gccacgtcgg ggagtcaggc aactatggat
     3001 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     3061 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     3121 atctaggtga agatcctttt tgataatctc atgctctagt tccactgagc gtcagacccc
     3181 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     3241 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     3301 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     3361 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     3421 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     3481 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     3541 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagcattga
     3601 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     3661 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     3721 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3781 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3841 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3901 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcaacgc gtgagcccac
     3961 cagctccgta agttcgggtg ctgtgtggct cgtacccgcg cattcaggcg gcagggggtc
     4021 taacgggtct aaggcggcgt gtacggccgc cacagcggct cttagcggcc cggaaacgtc
     4081 ctcgaaacga cgcatgtgtt cctcctggtt ggtacaggtg gttgggggtg ctcggctgtc
     4141 gctggtgttt catcatcagg gctcgacggg agagcggggg agtgtgcagt tgtggggtgg
     4201 cccctcagcg aaatatctga cttggagctc gtgtcggacc atacaccggt gattaatcgt
     4261 ggtttattat caagcgtgag ccacgtcgcc gacgaatttg agcagctctg gctgccgtac
     4321 tggtccctgg caagcgacga tctgctcgag gggatctacc gccaaagccg cgcgtcggcc
     4381 ctaggccgcc ggtacatcga ggcgaaccca acagcgctgg caaacctgct ggtcgtggac
     4441 gtagaccatc cagacgcagc gctccgagcg ctcagcgccc gggggtccca tccgctgccc
     4501 aacgcgatcg tgggcaatcg cgccaacggc cacgcacacg cagtgtgggc actcaacgcc
     4561 cctgttccac gcaccgaata cgcgcggcgt aagccgctcg catacatggc ggcgtgcgcc
     4621 gaaggccttc ggcgcgccgt cgatggcgac cgcagttact caggcctcat gaccaaaaac
     4681 cccggccaca tcgcctggga aacggaatgg ctccactcag atctctacac actcagccac
     4741 atcgaggccg agctcggcgc gaacatgcca ccgccgcgct ggcgtcagca gaccacgtac
     4801 aaagcggctc cgacgccgct agggcggaat tgcgcactgt tcgattccgt caggttgtgg
     4861 gcctatcttc ccgccctcat gcggatctac ctgccgaccc ggaacgtgga cggactcggc
     4921 cgcgcgatct atgccgagtg ccacgcgcga aacgccgaat ttccgtgcaa cgacgtgtgt
     4981 cccggaccgc taccggacag cgaggtccgc gccatcgcca acagcatttg gcgttggatc
     5041 acaaccaagt cgcgcatttg ggcggacggg atcgtggtct acgaggccac actcagtgcg
     5101 cgccatgcgg ccatctcgcg gaagggcgca gcagcgcgca cggcggcgag cacagttgcg
     5161 cggcgcgcaa agtccgcgtc agccatggag gcattgctat gagcgacggc tacagcgacg
     5221 gctacagcga cggctacaac tggcagccga ctgtccgcaa aaagcggcgc gtgaccgccg
     5281 ccgaaggcgc tcgaatcacc ggactatccg aacgccacgt cgtccggctc gtggcgcagg
     5341 aacgcagcga gtggttcgcc gagcaggctg cacgccgcga acgcatccgc gcctatcacg
     5401 acgacgaggg ccactcttgg ccgcaaacgg ccaaacattt cgggctgcat ctggacaccg
     5461 ttaagcgact cggctatcgg gcgaggaaag agcgtgcggc agaacaggaa gcggctcaaa
     5521 aggcccacaa cgaagccgac aatccaccgc tgttctaacg caattgggga gcgggtgtcg
     5581 cgggggttcc gtggggggtt ccgttgcaac gggtcggaca ggtaaaagtc ctggtagacg
     5641 ctagttttct ggtttgggcc atgcctgtct cgttgcgtgt ttcgttgcgt ccgttttgaa
     5701 taccagccag acgagacggg gttctacgaa tcttggtcga taccaagcca tttccgctga
     5761 atatcgtgga gctcaccgcc agaatcggtg gttgtggtga tgtacgtggc gaactccgtt
     5821 gtagtgcttg tggtggcatc cgtggcgcgg ccgcggtacc agatctttaa a
//
