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LB-G vector (V009872)

Basic Vector Information

Vector Name:
LB-G
Antibiotic Resistance:
Ampicillin
Length:
5395 bp
Type:
Retroviral cloning vector
Replication origin:
ori
Source/Author:
Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.

LB-G vector Vector Map

LB-G5395 bp6001200180024003000360042004800T7 promoter5' LTRtRNA-Pro binding siteMMLV Psigag (truncated)pol regionEGFP3' LTRliteral polyAM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

LB-G vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_1604        5395 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral cloning vector LB-G, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.
  TITLE     Expanding the spectrum of genetic elements transferable by 
            retroviral vectors
  JOURNAL   DNA Cell Biol. 26 (11), 773-779 (2007)
  PUBMED    17824835
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Lorens JB, Bogenberger JM, Swift SE, Micklem DR, Bloe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2007) Biomedicine, University of Bergen, Jonas 
            Lies vei 91, Bergen 5009, Norway
REFERENCE   3  (bases 1 to 5395)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Cell 
            Biol."; date: "2007"; volume: "26"; issue: "11"; pages: "773-779"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-2007) Biomedicine, University of Bergen, Jonas Lies vei 91, 
            Bergen 5009, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   25..169
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    25..93
                     /label=5' R region
                     /note="5' R region"
     misc_feature    94..169
                     /label=U5 region
                     /note="U5 region"
     misc_binding    170..187
                     /label=tRNA-Pro binding site
                     /bound_moiety="tRNA-Pro"
     misc_feature    232..589
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             654..1070
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1080..1449
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             1547..2263
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     repeat_region   2323..2839
                     /label=3' LTR
                     /note="3' LTR"
     misc_feature    2323..2771
                     /label=U3 region
                     /note="U3 region"
     misc_feature    2772..2839
                     /label=3' R region
                     /note="3' R region"
     misc_feature    2839..2879
                     /label=literal polyA
                     /note="literal polyA"
     primer_bind     complement(2900..2916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2924..2940)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2948..2978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2993..3014)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4064..4921)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4922..5026)
                     /label=AmpR promoter

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