LB-G vector (V009872)

Basic Vector Information

      • Vector Name:
      • LB-G
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5395 bp
      • Type:
      • Retroviral cloning vector
      • Source/Author:
      • Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.

LB-G vector Vector Map

LB-G5395 bp6001200180024003000360042004800T7 promoter5' LTRMoMLV extended packaging signal (psi+)from MoMLV pol polyprotein; contains splice acceptorvertebrate consensus sequence for strong initiation of translation (Kozak, 1987)3' LTRliteral polyAM13 revlac operatorlac promoterCAP binding sitepMB1 ori (pUC)blaAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

LB-G vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                5395 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral cloning vector LB-G, complete sequence.
ACCESSION   EF394360
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.
  TITLE     Expanding the spectrum of genetic elements transferable by 
            retroviral vectors
  JOURNAL   DNA Cell Biol. 26 (11), 773-779 (2007)
  PUBMED    17824835
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Lorens JB, Bogenberger JM, Swift SE, Micklem DR, Bloe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2007) Biomedicine, University of Bergen, Jonas 
            Lies vei 91, Bergen 5009, Norway
REFERENCE   3  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Retroviral cloning vector LB-G"
                     /mol_type="other DNA"
                     /db_xref="taxon:421945"
     regulatory      1..24
                     /regulatory_class="promoter"
                     /note="T7 promoter"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   25..169
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    25..93
                     /label=5' R region
                     /note="5' R region"
     misc_feature    94..169
                     /label=U5 region
                     /note="U5 region"
     regulatory      169..1079
                     /regulatory_class="other"
                     /note="MoMLV extended packaging signal (psi+)"
     misc_binding    170..187
                     /label=tRNA-Pro binding site
                     /bound_moiety="tRNA-Pro"
     misc_structure  228..252
                     /label=packaging stem-loop A
                     /note="packaging stem-loop A"
     misc_feature    232..589
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     misc_structure  302..333
                     /label=packaging stem-loop B
                     /note="packaging stem-loop B"
     misc_structure  334..376
                     /label=packaging stem-loop C
                     /note="packaging stem-loop C"
     misc_structure  379..398
                     /label=packaging stem-loop D
                     /note="packaging stem-loop D"
     CDS             645..1070
                     /codon_start=1
                     /gene="gag"
                     /product="Pr65 Gag polyprotein"
                     /label=gag
                     /note="from Moloney murine leukemia virus"
                     /protein_id="ABN09913.1"
                     /translation="MGPGQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEW
                     PTFNVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVH
                     PKPPPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     gene            645..1070
                     /gene="gag"
                     /label=gag
     misc_difference 651..656
                     /gene="gag"
                     /label=6bp insert compared to MoMLV
                     /note="6bp insert compared to MoMLV"
     CDS             654..1070
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1080..1449
                     /note="from MoMLV pol polyprotein; contains splice 
                     acceptor"
     regulatory      1541..1550
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1547..2266
                     /codon_start=1
                     /gene="GFP"
                     /product="enhanced green fluorescent protein"
                     /label=GFP
                     /note="EGFP"
                     /protein_id="ABN09914.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            1547..2266
                     /gene="GFP"
                     /label=GFP
     repeat_region   2323..2839
                     /label=3' LTR
                     /note="3' LTR"
     misc_feature    2323..2771
                     /label=U3 region
                     /note="U3 region"
     misc_feature    2772..2839
                     /label=3' R region
                     /note="3' R region"
     misc_feature    2839..2879
                     /label=literal polyA
                     /note="literal polyA"
     primer_bind     complement(2900..2916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2924..2940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2948..2978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2993..3014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      3287..3999
                     /label=pMB1 ori (pUC)
                     /note="pMB1 ori (pUC)"
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4061..4921)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /function="ampicillin resistance"
                     /label=bla
                     /protein_id="ABN09912.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(4061..4921)
                     /gene="bla"
                     /label=bla
     promoter        complement(4922..5026)
                     /gene="bla"
                     /label=AmpR promoter

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