LOCUS       40924_1604        5395 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Retroviral cloning vector LB-G, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Blo M, Bogenberger JM, Swift SE, Micklem DR, Lorens JB.
  TITLE     Expanding the spectrum of genetic elements transferable by 
            retroviral vectors
  JOURNAL   DNA Cell Biol. 26 (11), 773-779 (2007)
  PUBMED    17824835
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Lorens JB, Bogenberger JM, Swift SE, Micklem DR, Bloe M.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-JAN-2007) Biomedicine, University of Bergen, Jonas 
            Lies vei 91, Bergen 5009, Norway
REFERENCE   3  (bases 1 to 5395)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5395)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "DNA Cell 
            Biol."; date: "2007"; volume: "26"; issue: "11"; pages: "773-779"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-JAN-2007) Biomedicine, University of Bergen, Jonas Lies vei 91, 
            Bergen 5009, Norway"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   25..169
                     /label=5' LTR
                     /note="5' LTR"
     misc_feature    25..93
                     /label=5' R region
                     /note="5' R region"
     misc_feature    94..169
                     /label=U5 region
                     /note="U5 region"
     misc_binding    170..187
                     /label=tRNA-Pro binding site
                     /bound_moiety="tRNA-Pro"
     misc_feature    232..589
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             654..1070
                     /codon_start=1
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
                     /translation="GQTVTTPLSLTLGHWKDVERIAHNQSVDVKKRRWVTFCSAEWPTF
                     NVGWPRDGTFNRDLITQVKIKVFSPGPHGHPDQVPYIVTWEALAFDPPPWVKPFVHPKP
                     PPPLPPSAPSLPLEPPRSTPPRSSLYPALTPSLGA"
     misc_feature    1080..1449
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     CDS             1547..2263
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     repeat_region   2323..2839
                     /label=3' LTR
                     /note="3' LTR"
     misc_feature    2323..2771
                     /label=U3 region
                     /note="U3 region"
     misc_feature    2772..2839
                     /label=3' R region
                     /note="3' R region"
     misc_feature    2839..2879
                     /label=literal polyA
                     /note="literal polyA"
     primer_bind     complement(2900..2916)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2924..2940)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2948..2978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2993..3014)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3302..3890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4064..4921)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4922..5026)
                     /label=AmpR promoter
ORIGIN
        1 gcatgctaat acgactcact ataggcgcca gtcctccgat tgactgagtc gcccgggtac
       61 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
      121 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt gggggctcgt
      181 ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg taagctggcc
      241 agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta tgcgcctgcg
      301 tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa ctgacgagtt
      361 cggaacaccc ggccgcaacc ctgggagacg tcccagggac ttcgggggcc gtttttgtgg
      421 cccgacctga gtccaaaaat cccgatcgtt ttggactctt tggtgcaccc cccttagagg
      481 agggatatgt ggttctggta ggagacgaga acctaaaaca gttcccgcct ccgtctgaat
      541 ttttgctttc ggtttgggac cgaagccgcg ccgcgcgtct tgtctgctgc agcatcgttc
      601 tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaatatgggc ccgggccaga
      661 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
      721 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
      781 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
      841 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
      901 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
      961 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     1021 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc cccatatggc
     1081 catatgagat cttatatggg gcacccccgc cccttgtaaa cttccctgac cctgacatga
     1141 caagagttac taacagcccc tctctccaag ctcacttaca ggctctctac ttagtccagc
     1201 acgaagtctg gagacctctg gcggcacgta ccaagaacaa ctggaccgac cggtgctcac
     1261 ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa cctagaacct
     1321 cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa agtagacggc
     1381 atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg tggaccatcc
     1441 tctagactgc cggatctcga ggcgcgccag tgtggtggta gggaattcaa agcttcgaat
     1501 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
     1561 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1621 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1681 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1741 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1801 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1861 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1921 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1981 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2041 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2101 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2161 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2221 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgctcgac
     2281 gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag accccacctg
     2341 taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa atacataact
     2401 gagaatagag aagttcagat caaggtcagg aacagatgga acagctgaat atgggccaaa
     2461 caggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggaacagc
     2521 tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     2581 aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc atcagatgtt
     2641 tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt
     2701 cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc
     2761 cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat
     2821 aaaccctctt gcagttgcaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaac
     2881 gcgtgaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     2941 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3001 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     3061 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     3121 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     3181 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     3241 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     3301 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     3361 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     3421 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     3481 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     3541 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     3601 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     3661 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     3721 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     3781 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     3841 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     3901 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     3961 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     4021 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     4081 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     4141 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     4201 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     4261 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     4321 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     4381 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     4441 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     4501 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     4561 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     4621 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     4681 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     4741 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     4801 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     4861 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     4921 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     4981 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     5041 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     5101 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     5161 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     5221 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag
     5281 agcagattgt actgagagtg caccatatcg acgctctccc ttatgcgact cctgcattag
     5341 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggt
//
