Basic Vector Information
L398-2 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
L398-2 vector Sequence
LOCUS 40924_1574 8967 bp DNA circular SYN 17-DEC-2018
DEFINITION Expression vector L398-2, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8967)
AUTHORS Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV,
Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U,
Bosch TC, Khalturin K.
TITLE Blood System Formation in the Urochordate Ciona intestinalis
Requires the Variable Receptor vCRL1
JOURNAL Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
PUBMED 22513285
REFERENCE 2 (bases 1 to 8967)
AUTHORS Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV,
Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
Bosch TCG., Khalturin K.
TITLE Direct Submission
JOURNAL Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts
University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein
24118, Germany
REFERENCE 3 (bases 1 to 8967)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8967)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol.
Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(16-FEB-2011) Zoological Institute, Christian-Albrechts University
Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118,
Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8967
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 2448..3161
/codon_start=1
/label=GFP
/note="Aequorea victoria green fluorescent protein"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
promoter complement(6124..6142)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(6152..6168)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 6310..6765
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 6791..6895
/label=AmpR promoter
CDS 6896..7753
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7927..8515
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 8803..8824
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8839..8869
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8877..8893
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8901..8917
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 8938..8956
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
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