LOCUS       40924_1574        8967 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector L398-2, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8967)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich BV, Samoilovich MP, Satou Y, Kruss M, Gelhaus C, Kurn U, 
            Bosch TC, Khalturin K.
  TITLE     Blood System Formation in the Urochordate Ciona intestinalis 
            Requires the Variable Receptor vCRL1
  JOURNAL   Mol. Biol. Evol. 29 (10), 3081-3093 (2012)
  PUBMED    22513285
REFERENCE   2  (bases 1 to 8967)
  AUTHORS   Sommer F, Awazu S, Anton-Erxleben F, Jiang D, Klimovich AV, 
            Klimovich VB, Samoilovich MP, Satou Y, Kruess M, Gelhaus C, Kuern U,
            Bosch TCG., Khalturin K.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-FEB-2011) Zoological Institute, Christian-Albrechts 
            University Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 
            24118, Germany
REFERENCE   3  (bases 1 to 8967)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8967)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Biol. 
            Evol."; date: "2012"; volume: "29"; issue: "10"; pages: "3081-3093"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (16-FEB-2011) Zoological Institute, Christian-Albrechts University 
            Kiel, Am Botanischen Garten 9, Kiel, Schleswig-Holstein 24118, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8967
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             2448..3161
                     /codon_start=1
                     /label=GFP
                     /note="Aequorea victoria green fluorescent protein"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     promoter        complement(6124..6142)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6152..6168)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6310..6765
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6791..6895
                     /label=AmpR promoter
     CDS             6896..7753
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7927..8515
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    8803..8824
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8839..8869
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8877..8893
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8901..8917
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8938..8956
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggtaccaagt gcttgaaatg ctaaatgttt tcaatttttc gccattaaga caagcctaca
       61 caaatgcttc tataaattat gccaagcacg ttagcagctt ctacgagccc caaccactat
      121 taattcgaac agcatgtttt tttgcagtgc gcaatgttta acacactata ttatcaatac
      181 tactaaagat aacacatacc aatgcatttc gtctcaaaga gaattttatt ctcttcacga
      241 cgaaaaaaaa agttttgctc tatttccaac aacaacaaaa atatgagtaa tttattcaaa
      301 cggtttgctt aagagataag aaaaaagtga ccactattaa ttcgaacgcg gcgtaagctt
      361 accttaatct caagaagagc aaaacaaaag caactaatgt aacggaatca ttatctagtt
      421 atgatctgca aataatgctg cagggaatgt aacttgcttt accatcgcgt ggtgggaaaa
      481 cgacagtcgt tatagcacga gtgttcatac acctcgtgcc agcttacgag ctaccatata
      541 tgttgtgggc gaataaaggt tttataaata taacaggttt tataaatata acaacgccat
      601 tttaaagtcg gttacataat tctgtaacta gttcaaattg aacggtaaac gtaaataaaa
      661 accttgaccg tcttacccaa ttatataaaa acactttgaa cgctttttag gatggaaggg
      721 tatggccatg ctagataatt ctgtggacca tctcaaccca acctattaca gaacggtcgt
      781 aataatgaaa atggatacca tttttaggca tatagactga ttcctctact ttctagaaac
      841 gtaagcagta tacacagaaa aaatgaagtg tgattctgtg caattaaacc gttctaaatt
      901 catagccgac tgaatttcta attaagtgaa tgtctgacct agatttattg ttaagtttag
      961 caccaaatct gagccagcga taagcagtct aattaaattg gctgctggcg ataaaatagg
     1021 tcatcctgaa aaatcgtttg cgcctttatt taaaatatag tagagtgggg aaagacggga
     1081 catcttatcg ttctattttc tcgtcccatt tcgtagtaaa caaagaacat tcaaaaaata
     1141 taaaaccata acttcaaaac ttcaatagac cgttgtcaac tgtttaaaac acagtaagag
     1201 aatttggata ttatgtgcta aaggtgtccc atctcccccc accctactat atctgtttat
     1261 agttctgtgg ggtaagatga gataccgtta acacctaaac atttttactt taaacaatca
     1321 accacgtttt ttatggtcgt gatgggcatg aggttacata attctgaaaa tattttttgc
     1381 ccccgaccaa aagacgcgaa gagtaaaaac atgtctcagc ttatattccc cacataaata
     1441 tatttttgta ctgtttggtg aatttataaa cttatattac catgcatata cgttatgtta
     1501 ctggtatttt ctcagtaggc aaattcattt gtccacgttt tataggtttt tatttatgat
     1561 ttttaaaatg ctaaaaatgt gggagggggt tgaaagtaca atacaaacac acaaaacaac
     1621 tcaaactaaa gatttatagt tatgctaatt cacctacaca atataacaag atgtgtaatg
     1681 caaccatgtg tttatgatga gcgctaacat attttgtaac cactcaaatt ccccgccaca
     1741 cgaggataat gaataggtga ctctgtagtc tgtacatctt agactgaaat aaagattata
     1801 aatctacgaa ataaaataat ttctgctcac tgattatact tctgttttat agattagaaa
     1861 ccgtttctaa taaatgacct aattcgctat acacacacgc tgtgcgcgag ataatcattc
     1921 tcgcaccccg tttattgtgt taaaattgcc gcctagattc acaaagcgtg acggctagag
     1981 ccagcaacgt gtcgccttca attacgcaac atccgggttg cgcaattctg gatataaaag
     2041 aactaacaaa gatgacgtag ctaccttttt cagttcagac ttacgaaaga ctcacgtgtc
     2101 ggcggtctac ttgtcctttt cgagctgtgg caatttggtg agtggttcta tcttatatct
     2161 gagtacatct ctaaggaatt atagtttgat tagttaagtt tttattgtta ggaaagatga
     2221 aatcattagg ttttacttag tttaagtatg ttagtactgg ttaggcgttt gaattattga
     2281 aaaactcagt tcgttaactg tagtagttct ggtagcttag caagtatacc ctgtatacgc
     2341 cttttggctt tttaacaata acttaaactt attttacagc aaatttctgt gcattcggtt
     2401 aaccccaacc ttccaaaatg aaagacaaga ctttaattaa tgaaaaaatg agtaaaggag
     2461 aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat gttaatgggc
     2521 acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa cttaccctta
     2581 aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc actactttct
     2641 gttatggtgt tcaatgcttt tcaagatacc cagatcatat gaaacggcat gactttttca
     2701 agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa gatgacggga
     2761 actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat agaatcgagt
     2821 taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg gaatacaact
     2881 ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc aaagttaact
     2941 tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat tatcaacaaa
     3001 atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg tccacacaat
     3061 ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt gagtttgtaa
     3121 cagctgctgg gattacacat ggcatggatg aactatacaa agcgatcgcg ttttaaaatt
     3181 ctgggcattt cacacttttt atacaatatt ggtatcactt tgttcttatt aaattcaaat
     3241 gataaagata atgcaatttt agcttgtgtt cgacagttat ttgatttgtt gaaattagga
     3301 gataaaataa ctataacttt cctgcatttt tcttcaataa cactgcataa ctgctcaaag
     3361 ggattgtacc caggcaaaaa atcacaataa tcaatacaaa ctttataccc atattcattc
     3421 tctaaacgat tcttaattaa cttagcaaaa tttatatcat cttttgcata tgatataaag
     3481 acgtcataat tatcattgag tgtaaaatta ctaacatgag ttatggagca ttgcaaatct
     3541 tgattgtcat tttcaataaa attgttatca ttaatcgtac gaaaccaatg gattgcatta
     3601 ataaagatca attactttat cagtctctgt catatcctat aatgagtcaa gtttcatttt
     3661 tacttaatcc tatttgtgat aataaagatt ggagagcctt agctggtgta tttgatttta
     3721 cgatggaaga agtgaatggt ttgttatctg cttgtgaccc aacaatggag ttatttaaat
     3781 attatgagtt gaaatatggc aaaaaagtta cattatcata tgttatagaa tcattaaata
     3841 atattggaag aaaagatgtt gtacaagata ttcttaaata cattaatgat aacaatttta
     3901 ttgaaaatga caatcaagat ttgcaatgct ccataactca tgttagtaat tttacactca
     3961 atgataatta tgacgtcttt atatcatatg caaaagatga tataaatttt gctaagttaa
     4021 ttaagaatcg tttagagaat gaatatgggt ataaagtttg tattgattat tgtgattttt
     4081 tgcctgggta caatcccttt gagcagttat gcagtgttat tgaagaaaaa tgcaggaaag
     4141 ttatagttat tttatctcct aatttcaaca aatcaaataa ctgtcgaaca caagctaaaa
     4201 ttgcattatc tttatcattt gaatttaata agaacaaagt gataccaata ttgtataaaa
     4261 agtgtgaaat gcccagaatt ttaaaacgaa ttctacatcg cgtgactgca gccaacgaca
     4321 ccgcccacca tcacttctgc agttttactc caccagcttg ctcacccttc taaccatttc
     4381 agcctagtta aacccccagt tcaatttgtc agccttttaa attgcgtcat gacatttcag
     4441 ttgttactgt gtgccgctct gtccacatgg tgtagttatg tttttcatta cccccaccga
     4501 aaacactttc gtaggaaaaa accctccgtt tcgtgccaac ttaaaagtgt tatttttgtt
     4561 tctagcgctg cttaacttat ttaaaatgac aagactatct atgattttaa accatggctt
     4621 tgaatggcgt acatgtatta ttgtgtttat attcagttaa accgtactat tactgcttgg
     4681 aaaattcagt tcattccttc accgaaggct ttaacgtggt tggttgcggt gttgtcaatt
     4741 tgctgctgga aataaagctt ctgttaagtg tatactgtct cttggtttgc aagattatta
     4801 tggatccaga tgttgtatat acaagagtga agatttagcg tttttatgtg gtaaaacgcc
     4861 catggtgatc tatttacagt ttttaagttg agctgatcaa ttttcacatg atgatcctga
     4921 acgaccaggg gcacaaaggc atttgcagat tgtcccatct ctcactacga ccgatctacc
     4981 actattttca caacggccag tacatttcga agtctggtat ttagcgaagt agtgttcaat
     5041 tgccaaagcc atgtttctct gcttcgctct gtctgggatg accgatgata tgggtcgcag
     5101 ctgtaaaatt acaaatagtc agtttttctg acgaaagtga atgttgatta cattcaaatg
     5161 aaagtcgttg atggctaatg ccatcgttca tacgctttaa ttagcagatt atgtggttat
     5221 attagatatc tttaccagta atattgagaa cgccaaggta aaatctagtt ttttgatact
     5281 taatgaacag ccatattaaa agccatacat gaaaaaaaaa ttaaaaaaaa catacataaa
     5341 ctctcataag ctgacacagt aggtcgtggc tgacacgagg tgtatgaaac aaaacgctcg
     5401 tgttttaacg actgttgttg caccgtcacg cgagaataaa gtaacttcca ttcatacatg
     5461 atacagttct taccttgaaa tcgatgactg cagggctaag tttaactgat gctgtccagt
     5521 tttcgtaagc atttgttgta agtcggtttc tagcaaagaa actgagagca ctagctgtat
     5581 gagactgccc accaatcaca ttcgatataa cttccttagc agccattgta aaacttccta
     5641 aatattagta aataacgaat aataatgtgt acatgactct gcttatcagt tagattgggc
     5701 gcgccatcag tttaaacgaa tgcggccgcc accgcgggac gttacacaat tctaatatta
     5761 attaaattat tgttttaagt atgatagtaa atcacattac gccgcgttcg aattaatagt
     5821 ggtcactttt ttcttatctc ttaagcaaac cgtttgaata aattactcat atttttgttg
     5881 ttgttggaaa tagagcaaaa cttttttttt cgtcgtgaag agaataaaat tctctttgag
     5941 acgaaatgca ttggtatgtg ttatctttag tagtattgat aatatagtgt gttaaacatt
     6001 gcgcactgca aaaaaaacat gctgttcgaa ttaatagtgg ttggggctcg tagaaaacga
     6061 aaaatatctt aagctagcat agagaatgga gcaaaactca atttgatgcg agctccaatt
     6121 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6181 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6241 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6301 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6361 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6421 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6481 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6541 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6601 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     6661 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     6721 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     6781 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     6841 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     6901 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     6961 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7021 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7081 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7141 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7201 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7261 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7321 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7381 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7441 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7501 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7561 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7621 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     7681 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     7741 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     7801 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7861 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7921 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7981 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8041 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     8101 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8161 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8221 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8281 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8341 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8401 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8461 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8521 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     8581 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     8641 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     8701 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     8761 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     8821 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     8881 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     8941 taaccctcac taaagggaac aaaagct
//
