Basic Vector Information
krt4-sfGFP-tVE1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
krt4-sfGFP-tVE1 vector Sequence
LOCUS 40924_1504 8416 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector krt4-sfGFP-tVE1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8416)
AUTHORS Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M,
Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
TITLE Gain-of-function mutations of mau/DrAqp3a influence zebrafish
pigment pattern formation through the tissue environment
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 8416)
AUTHORS Eskova A.
TITLE Direct Submission
JOURNAL Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for
Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE 3 (bases 1 to 8416)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 8416)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(28-JAN-2016) e-CNV group, Max Planck Institute for Developmental
Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..8416
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1061..1077
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1097..1117
/label=attB4
/note="core recombination site for the Gateway(R) BP
reaction"
promoter 1119..1137
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 1163..1179
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
regulatory 1195..3433
/label=krt4
/note="krt4"
/regulatory_class="promoter"
CDS 3461..4177
/label=superfolder GFP
/note="GFP variant that folds robustly even when fused to
poorly folded proteins (Pedelacq et al., 2006)"
polyA_signal 5116..5250
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(5313..5333)
/label=attB3
/note="core recombination site for the Gateway(R) BP
reaction"
primer_bind complement(5341..5357)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
polyA_signal complement(5373..5507)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6179..6195)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(6232..6250)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6271..6287)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(6295..6311)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6319..6349)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(6364..6385)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6673..7261)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7435..8292)
/label=AmpR
/note="beta-lactamase"
promoter complement(8293..8397)
/label=AmpR promoter
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