LOCUS       40924_1504        8416 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector krt4-sfGFP-tVE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, 
            Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 8416)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8416)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental 
            Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8416
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1097..1117
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        1119..1137
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1163..1179
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      1195..3433
                     /label=krt4
                     /note="krt4"
                     /regulatory_class="promoter"
     CDS             3461..4177
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     polyA_signal    5116..5250
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5313..5333)
                     /label=attB3
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     primer_bind     complement(5341..5357)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(5373..5507)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6179..6195)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6232..6250)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6271..6287)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6295..6311)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6319..6349)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6364..6385)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6673..7261)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7435..8292)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8293..8397)
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggta atacgactca ctatagggcg
     1141 aattgggtac cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattcc
     1201 cttcccttct acttttgacg tccttttaag agcttgtgca tgaaagcaga tttggagctg
     1261 attactcatc tcaaacaccc atacaaaggg atgattgccg taccatgatc tcacaccttt
     1321 cacacctggt ttatactatg atagttgtag acgattgcgt aatgctatta aatgcccatc
     1381 agtgctggct gtgacaccca actgctgcca tttcgtgttg acttgcacga gaaatgagaa
     1441 attgtctgac tatgcagggt gtctatgcgt gggaacattt atcagtggtc attaaatact
     1501 atagtttaca gttagaccaa agtgtgctgt atttttgtgt tagcttagct gcagtttttg
     1561 tgtgtgaagt aacaaatgac aaatactcaa actattgtaa ttaagtagtt tttctcagaa
     1621 attgtaattt actaagtagt ttaaaaatgt gtacttttac tttcccttga gtacattttt
     1681 agtgcagtgt tggtactttt atttcacttc cttccttcaa cctgcagtca ctactttatt
     1741 tattcttgtc tatgtggatt agacaaatca gtcctgtgat tcctgtccaa tcaaattgca
     1801 catagaaggt aaatcacatc ataatgaact accttaagac atgggccatt tataattgca
     1861 gcaaactgtt tgccagcatt aaaagaagat gtcaaaaata tttacacgca ttaacccaga
     1921 gactgcttag atgcatgtca ctgatgagaa gatgatggat gtttactgta tgatgaccga
     1981 aataacttta aacgcacaca agacggcaca agacgtcaac atggcgttag gttgacgttg
     2041 taccccaacg cagtggggac gttgcatttt gtttagaaat gaaaattagg ttgacgtcag
     2101 aactcaacgt caggtcgatg tcaatgttca acatccaatc taaaatcata tatcaatgtc
     2161 taatgatgtt acagcttgat gttatgcgga tgttacccct atgacgtcta tcagacgttg
     2221 gattatggtt gccatacctg atgaataaat gtcattattt gacgttggtt taagatgttg
     2281 gttcgacatt ggattttggt cgctttccaa cacaacctaa atccaccaaa tattaacttc
     2341 ctatgacatc gttattggac gtcaaaataa caatatcctt agatgctggc tagactttga
     2401 atttaggtca ccacaaccta tatttaacct aatattaaca tcttatgatg ttgtgtgcct
     2461 gctgggcaat aactaaatgc actacagaat gttacgttta cacacatgta aattacatgt
     2521 aaatgcatca gcttttcaca gcataatact cactacttac tactcttgag tacttttaaa
     2581 aaagctactt ttcactcata ctttgagtaa tatttacaac tgatactttt actcgcacta
     2641 catttttagg catgtattga tatttttact atgatttttc agtactcttt ccactactgc
     2701 agccctcccc atacataatc gtatgtttac acatatggtg gagtttagag ccataatcta
     2761 cattagcttt gttagccgct agcattactg tgcagaattg tgtgtgtgca cattttccaa
     2821 tatcaataca gaaggaaact gtgttccctg ttcccttgta aatctcaaca atgcaactgt
     2881 tcagctcagg gggaaaaatg ccctgccaga tccaaacggc tggcaaaagt gaatggaaaa
     2941 aagcctttca ttaatgtgaa agttgctgcg cgccccaccc agataaaaag agcagaggtt
     3001 aacatgctct ctacggctgt ccagccaacc agatactgag gcagaaacac acccgctggc
     3061 agatggtgag agctacactg tcttttccag agtttctact ggaatgcctg tcctcaagtc
     3121 tcaagcctct ccttgcattc tctcattcca cctggggcaa agccccaggc tgggtgtgac
     3181 aacatttatc ttaccacttt ctctctgtac ctgtctaaca ggtagggtgt gtgtgagagt
     3241 gcgtatgtgt gcaagtgcgt gtgtgtgtga gagcagtcag ctccaccctc tcaagagtgt
     3301 gtataaaatt ggtcagccag ctgctgagag acacgcagag ggactttgac tctcctttgt
     3361 gagcaacctc ctccactcac tcctctctca gagagcactc tcgtacctcc ttctcagcaa
     3421 ctcaaagaca caggatccca tcgattcgaa tagcgccacc atggtgtcta aaggagagga
     3481 gctgttcaca ggcgtggtgc caatcctggt ggagctggat ggagacgtga acggccacaa
     3541 gttcagcgtg agaggcgagg gagagggaga cgccacaaac ggcaagctga cactgaaatt
     3601 catctgcaca acaggcaaac tgcctgtgcc ttggccaacc ctggtgacaa ccctgacata
     3661 cggagtgcag tgctttagca gataccctga tcacatgaaa cagcacgatt tcttcaagag
     3721 cgccatgcct gagggctacg tgcaggagag aaccatcagc ttcaaggatg acggaaccta
     3781 caagacaaga gccgaggtga agtttgaggg agatacactg gtgaacagaa tcgagctgaa
     3841 aggcatcgat ttcaaagagg atggcaacat cctgggacac aaactggagt acaacttcaa
     3901 cagccacaac gtgtacatca cagccgataa acagaagaac ggcatcaaag ccaacttcaa
     3961 gatcagacac aatgtggagg atggatctgt gcagctggcc gatcactacc agcagaacac
     4021 acctatcgga gacggcccag tgctgctgcc agataaccac tacctgagca cacagagcgt
     4081 gctgtctaaa gaccctaacg agaagagaga tcacatggtg ctgctggagt ttgtgacagc
     4141 cgccggaatc accctgggca tggatgagct gtacaaatcc ggactcagat ctatgggttg
     4201 gcagaaaagc gttctggata agcttgcgca gactttccaa atccgcaaca agttactgcg
     4261 ccagggattg gctgaatgct taggaactct catcctggtg atgtttggct gtggttcatt
     4321 ggcccagttg aaactaagcg aaggttctca tggtctcttt ctcactgcaa accttgcttt
     4381 tgggtttggt gctactcttg gaatcttggt ttgcggccag gtgtcaggcg gacatttaaa
     4441 tcctgctgtt acatttgctc tctgcctctt gggaagagaa aaatggagaa agtttcctgt
     4501 gtactttctg ttccaaacac tcggatcctt cttgggtgct gctattatct ttgccgaata
     4561 ccatgacgca atttatgatt atgctggaga atcaaatgag ttgcttgtac tgggtgaaaa
     4621 agaaacagct gggatttttg ctacataccc aagcaaatat ctcacccccc taaatggatt
     4681 ttttgaccag gtgataggca cagcatccct gattgtgtgc atcctggcca ttgtggaccc
     4741 ctacaacaac ccgatccctc aaggtcttga ggccttcaca gtgggattca gcgtccttat
     4801 cattggtctc tccatgggct tcaattctgg ctatgcagta aacccagctc aagattttgg
     4861 acctcgtctt tttactgcca tggctggttg gggtagtgaa gtcttcacag ccagggatta
     4921 ttggtttttg gtgcccatct ttgctccgtt cattggagcc gttattggtg tgattgtgta
     4981 ccagctgatg gtgggatggc atgtggaagg agaggcacga gataagaaag ctaaagctag
     5041 agaggaggtg atgaacctca atgacgtcgc cagcaaggaa tgagaattct gcagtcgacg
     5101 gtaccgcggg cccgggatcc agacatgata agatacattg atgagtttgg acaaaccaca
     5161 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     5221 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     5281 caggttcagg gggaggtgtg ggaggttttt tccaacttta ttatacatag ttgataattc
     5341 actggccgtc gttttacggt accatcgatg atgatccaga catgataaga tacattgatg
     5401 agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg
     5461 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
     5521 gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa
     5581 acctctacaa atgtggtatg gctgattatg atcctctaga tcagatctgc gaagatacgg
     5641 ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag ctgctgatga
     5701 atcacatact tcctccattt tcttccactg attgactgtt ataatttccc taatttccag
     5761 gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca aaggaccaat
     5821 gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag aataaataac
     5881 atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca agggaaaata
     5941 gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt gtaaggagta
     6001 aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat tgatttttaa
     6061 ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta atcaagtaaa
     6121 attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa gcccagcaga
     6181 tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt ccctttagtg
     6241 agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     6301 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     6361 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     6421 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     6481 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     6541 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     6601 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     6661 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     6721 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     6781 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     6841 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6901 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6961 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7021 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7081 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7141 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     7201 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     7261 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     7321 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     7381 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     7441 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     7501 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     7561 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     7621 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     7681 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     7741 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     7801 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     7861 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7921 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7981 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8041 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8101 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     8161 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     8221 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     8281 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     8341 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     8401 atttccccga aaagtg
//
