JSC vector (V009903)

Basic Vector Information

      • Vector Name:
      • JSC
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4566 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Sepulveda J, Shoemaker CB.

JSC vector Vector Map

JSC4566 bp600120018002400300036004200lac promoterpelB leadermulti-cloning siteE-tagpIII fusion proteinM13-UPf1 oriAmpR promoterbeta lactamaseori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

JSC vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4566 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector JSC, complete sequence.
ACCESSION   EU109715
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Sepulveda J, Shoemaker CB.
  TITLE     Design and testing of PCR primers for the construction of scFv 
            libraries representing the immunoglobulin repertoire of rats
  JOURNAL   J. Immunol. Methods 332 (1-2), 92-102 (2008)
  PUBMED    18242637
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Shoemaker CB, Sepulveda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2007) Biomedical Science, Tufts Cummings School of
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA
REFERENCE   3  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Cloning vector JSC"
                     /mol_type="other DNA"
                     /db_xref="taxon:467236"
     regulatory      228..442
                     /regulatory_class="promoter"
                     /note="lac promoter"
     protein_bind    330..351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        366..396
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..420
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    443..508
                     /label=pelB leader
                     /note="pelB leader"
     CDS             443..508
                     /codon_start=1
                     /gene="pelB (fragment)"
                     /product="leader peptide for secretion"
                     /label=pelB signal sequence
                     /translation="MKYLLPTAAAGLLLLAAQPAMA"
     primer_bind     457..482
                     /label=ARAHF
                     /note="ARAHF"
     misc_feature    508..593
                     /label=multi-cloning site
                     /note="multi-cloning site"
     misc_feature    593..631
                     /label=E-tag
                     /note="E-tag"
     misc_feature    641..1855
                     /label=pIII fusion protein
                     /note="pIII fusion protein"
     primer_bind     1862..1879
                     /label=M13-UP
                     /note="M13-UP"
     primer_bind     complement(1862..1878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2091..2546
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     rep_origin      2091..2490
                     /label=M13 origin
                     /note="M13 origin"
     promoter        2828..2932
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2933..3793
                     /codon_start=1
                     /product="beta lactamase"
                     /label=beta lactamase
                     /protein_id="ABV03162.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      3984..4566
                     /label=ColE1 origin
                     /note="ColE1 origin"

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