Basic Vector Information
JSC vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
JSC vector Sequence
LOCUS 40924_1444 4566 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector JSC, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4566)
AUTHORS Sepulveda J, Shoemaker CB.
TITLE Design and testing of PCR primers for the construction of scFv
libraries representing the immunoglobulin repertoire of rats
JOURNAL J. Immunol. Methods 332 (1-2), 92-102 (2008)
PUBMED 18242637
REFERENCE 2 (bases 1 to 4566)
AUTHORS Shoemaker CB, Sepulveda J.
TITLE Direct Submission
JOURNAL Submitted (22-AUG-2007) Biomedical Science, Tufts Cummings School of
Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA
REFERENCE 3 (bases 1 to 4566)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 4566)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "J. Immunol.
Methods 332 (1-2), 92-102 (2008)"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(22-AUG-2007) Biomedical Science, Tufts Cummings School of
Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4566
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 330..351
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 366..396
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 404..420
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
sig_peptide 443..508
/label=pelB signal sequence
/note="leader peptide for secretion"
misc_feature 508..593
/label=multi-cloning site
/note="multi-cloning site"
misc_feature 593..631
/label=E-tag
/note="E-tag"
CDS 641..1852
/codon_start=1
/label=M13 gene III
/note="pIII"
/translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
SGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENALQSD
AKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFR
QYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANIL
RNKES"
primer_bind complement(1862..1878)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2091..2546
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2828..2932
/label=AmpR promoter
CDS 2933..3790
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3964..4552
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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