LOCUS       40924_1444        4566 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector JSC, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Sepulveda J, Shoemaker CB.
  TITLE     Design and testing of PCR primers for the construction of scFv 
            libraries representing the immunoglobulin repertoire of rats
  JOURNAL   J. Immunol. Methods 332 (1-2), 92-102 (2008)
  PUBMED    18242637
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Shoemaker CB, Sepulveda J.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2007) Biomedical Science, Tufts Cummings School of
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA
REFERENCE   3  (bases 1 to 4566)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4566)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J. Immunol.
            Methods 332 (1-2), 92-102 (2008)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-AUG-2007) Biomedical Science, Tufts Cummings School of 
            Veterinary Medicine, 200 Westboro Rd., North Grafton, MA 01536, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4566
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    330..351
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        366..396
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    404..420
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     sig_peptide     443..508
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     misc_feature    508..593
                     /label=multi-cloning site
                     /note="multi-cloning site"
     misc_feature    593..631
                     /label=E-tag
                     /note="E-tag"
     CDS             641..1852
                     /codon_start=1
                     /label=M13 gene III
                     /note="pIII"
                     /translation="TVESCLAKPHTENSFTNVWKDDKTLDRYANYEGCLWNATGVVVCT
                     GDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGGTKPPEYGDTPIPGYTYINPL
                     DGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQGALTVYTGTVTQGTDPVKTY
                     YQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQGQSSDLPQPPVNAGGGSGGG
                     SGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKMANANKGAMTENADENALQSD
                     AKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGSNSQMAQVGDGDNSPLMNNFR
                     QYLPSLPQSVECRPYVFGAGKPYEFSIDCDKINLFRGVFAFLLYVATFMYVFSTFANIL
                     RNKES"
     primer_bind     complement(1862..1878)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2091..2546
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2828..2932
                     /label=AmpR promoter
     CDS             2933..3790
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3964..4552
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
       61 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
      121 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
      181 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agggtacccg ataaaagcgg
      241 cttcctgaca ggaggccgtt ttgttttgca gcccacctct ggcacgacag gtttcccgac
      301 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
      361 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
      421 ttgaattcag gaggaattta aaatgaaata cctattgcct acggcagccg ctggattgtt
      481 attactcgcg gcccaaccgg ccatggccgg cccgggagcg gccgctggtg gaggcggttc
      541 aggcggaggt ggctctggcg gtggcggatc ggcgcgccag gcctgcacta gtggtgcgcc
      601 ggtgccgtat ccggatccgc tggaaccgcg tgccgcatag actgttgaaa gttgtttagc
      661 aaaacctcat acagaaaatt catttactaa cgtctggaaa gacgacaaaa ctttagatcg
      721 ttacgctaac tatgagggct gtctgtggaa tgctacaggc gttgtggttt gtactggtga
      781 cgaaactcag tgttacggta catgggttcc tattgggctt gctatccctg aaaatgaggg
      841 tggtggctct gagggtggcg gttctgaggg tggcggttct gagggtggcg gtactaaacc
      901 tcctgagtac ggtgatacac ctattccggg ctatacttat atcaaccctc tcgacggcac
      961 ttatccgcct ggtactgagc aaaaccccgc taatcctaat ccttctcttg aggagtctca
     1021 gcctcttaat actttcatgt ttcagaataa taggttccga aataggcagg gtgcattaac
     1081 tgtttatacg ggcactgtta ctcaaggcac tgaccccgtt aaaacttatt accagtacac
     1141 tcctgtatca tcaaaagcca tgtatgacgc ttactggaac ggtaaattca gagactgcgc
     1201 tttccattct ggctttaatg aggatccatt cgtttgtgaa tatcaaggcc aatcgtctga
     1261 cctgcctcaa cctcctgtca atgctggcgg cggctctggt ggtggttctg gtggcggctc
     1321 tgagggtggc ggctctgagg gtggcggttc tgagggtggc ggctctgagg gtggcggttc
     1381 cggtggcggc tccggttccg gtgattttga ttatgaaaaa atggcaaacg ctaataaggg
     1441 ggctatgacc gaaaatgccg atgaaaacgc gctacagtct gacgctaaag gcaaacttga
     1501 ttctgtcgct actgattacg gtgctgctat cgatggtttc attggtgacg tttccggcct
     1561 tgctaatggt aatggtgcta ctggtgattt tgctggctct aattcccaaa tggctcaagt
     1621 cggtgacggt gataattcac ctttaatgaa taatttccgt caatatttac cttctttgcc
     1681 tcagtcggtt gaatgtcgcc cttatgtctt tggcgctggt aaaccatatg aattttctat
     1741 tgattgtgac aaaataaact tattccgtgg tgtctttgcg tttcttttat atgttgccac
     1801 ctttatgtat gtattttcga cgtttgctaa catactgcgt aataaggagt cttaagaatt
     1861 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1921 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1981 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     2041 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     2101 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2161 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2221 ttttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2281 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     2341 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     2401 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     2461 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     2521 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     2581 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2641 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2701 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2761 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2821 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2881 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2941 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3001 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3061 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3121 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3181 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3241 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3301 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3361 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3421 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3481 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3541 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3601 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3661 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3721 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3781 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3841 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3901 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3961 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4021 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4081 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4141 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4201 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4261 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4321 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4381 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4441 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4501 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4561 caacgc
//
