Basic Vector Information
JN100 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
JN100 vector Sequence
LOCUS 40924_1439 6460 bp DNA circular SYN 17-DEC-2018
DEFINITION Expression vector JN100, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6460)
AUTHORS Nikodinovic J, Priestley ND.
TITLE A second generation snp-derived Escherichia coli-Streptomyces
shuttle expression vector that is generally transferable by
conjugation
JOURNAL Plasmid 56 (3), 223-227 (2006)
PUBMED 16806469
REFERENCE 2 (bases 1 to 6460)
AUTHORS Nikodinovic J, Priestley ND.
TITLE Direct Submission
JOURNAL Submitted (18-NOV-2005) Chemistry, University of Montana, 32 Campus
Dr, Missoula, MT 59812, USA
REFERENCE 3 (bases 1 to 6460)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 6460)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
date: "2006"; volume: "56"; issue: "3"; pages: "223-227"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(18-NOV-2005) Chemistry, University of Montana, 32 Campus Dr,
Missoula, MT 59812, USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6460
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS complement(191..1561)
/codon_start=1
/product="pIJ101 replication protein"
/label=pIJ101 replication protein
/protein_id="ABC02231.1"
/translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
oriT complement(2656..2765)
/direction=LEFT
/label=oriT
/note="incP origin of transfer"
primer_bind 2964..2980
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(3035..3799)
/label=ApmR
/note="aminoglycoside 3-N-acetyltransferase type IV"
primer_bind complement(3990..4006)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(4014..4030)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4038..4068)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(4083..4104)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4392..4980)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5058..5215
/note="multiple cloning site; MCS"
regulatory complement(5215..5255)
/label=snpA promoter
/note="snpA promoter"
/regulatory_class="promoter"
CDS 5473..6414
/codon_start=1
/gene="snpR"
/product="SnpR"
/label=snpR
/protein_id="ABC02232.1"
/translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
LAEAYGEVARQAPAYREWLERSGSGALVPALP"
gene 5473..6414
/gene="snpR"
/label=snpR
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