LOCUS       40924_1439        6460 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector JN100, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6460)
  AUTHORS   Nikodinovic J, Priestley ND.
  TITLE     A second generation snp-derived Escherichia coli-Streptomyces 
            shuttle expression vector that is generally transferable by 
            conjugation
  JOURNAL   Plasmid 56 (3), 223-227 (2006)
  PUBMED    16806469
REFERENCE   2  (bases 1 to 6460)
  AUTHORS   Nikodinovic J, Priestley ND.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-NOV-2005) Chemistry, University of Montana, 32 Campus 
            Dr, Missoula, MT 59812, USA
REFERENCE   3  (bases 1 to 6460)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6460)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid"; 
            date: "2006"; volume: "56"; issue: "3"; pages: "223-227"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-NOV-2005) Chemistry, University of Montana, 32 Campus Dr, 
            Missoula, MT 59812, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(191..1561)
                     /codon_start=1
                     /product="pIJ101 replication protein"
                     /label=pIJ101 replication protein
                     /protein_id="ABC02231.1"
                     /translation="MDPASGVIVAQTAAGTSVVLGLMRCGRIWLCPVCAATIRHKRAEE
                     ITAAVVEWIKRGGTAYLVTFTARHGHTDRLADLMDALQGTRKTPDSPRRPGAYQRLITG
                     GTWAGRRAKDGHRAADREGIRDRIGYVGMIRATEVTVGQINGWHPHIHAIVLVGGRTEG
                     ERSAKQIVATFEPTGAALDEWQGHWRSVWTAALRKVNPAFTPDDRHGVDFKRLETERDA
                     NDLAEYIAKTQDGKAPALELARADLKTATGGNVAPFELLGRIGDLTGGMTEDDAAGVGS
                     LEWNLSRWHEYERATRGRRAIEWTRYLRQMLGLDGGDTEADDLDLLLAADADGGELRAG
                     VAVTEDGWHAVTRRALDLEATRAAEGKDGNEDAAAVGERVREVLALADAADTVVVLTAG
                     EVAEAYADMLAALAQRREEATARRRREQDDDQDDDADDRQERAARHIARLASGPTSH"
     oriT            complement(2656..2765)
                     /direction=LEFT
                     /label=oriT
                     /note="incP origin of transfer"
     primer_bind     2964..2980
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3035..3799)
                     /label=ApmR
                     /note="aminoglycoside 3-N-acetyltransferase type IV"
     primer_bind     complement(3990..4006)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4014..4030)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4038..4068)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4083..4104)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4392..4980)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5058..5215
                     /note="multiple cloning site; MCS"
     regulatory      complement(5215..5255)
                     /label=snpA promoter
                     /note="snpA promoter"
                     /regulatory_class="promoter"
     CDS             5473..6414
                     /codon_start=1
                     /gene="snpR"
                     /product="SnpR"
                     /label=snpR
                     /protein_id="ABC02232.1"
                     /translation="MELEVRHLRALCAIADTGSLHRAARQLGVTQPSLSTQLRRIEHEL
                     GGALFVRARTGCRPTPLGRLVLSRARPLVAELRSLVSEARAAAVGGRQLRVGSTASRAL
                     AGWLRRLRRHWQEPTLHMDVSANALLRMVADGHLDVAFVHEVEGSPLRVPEGLRVRVLV
                     QREPQFVCLPADHPAAAKPSYASPTWPTTRWMIDPTVDGEWDAVRRVLRAEGLDPRILH
                     GDYHTAASLVATGEVVTVVQPTSPSRAETAVRRLHGDPLGVRLLLAARTDTELEGVYPD
                     LAEAYGEVARQAPAYREWLERSGSGALVPALP"
     gene            5473..6414
                     /gene="snpR"
                     /label=snpR
ORIGIN
        1 ctgggggggg acggcggtgc gggggatgca gcgggtcggc ggtaccgcct gcacgaagtg
       61 ccggcggggc cgaccccggg cgagtaatcc caggattact cccgcggctt cgaccccggc
      121 cgccgtcgcc gcgtacgtca ccgacccccg ccgtacgtca ccgggatgac gtacggcggg
      181 ggggagcgag ttagtgcgaa gtgggcccac ttgcgagccg ggcgatgtgc cgggcggccc
      241 gctcctggcg gtcgtcggcg tcgtcgtcct ggtcgtcgtc ctgctctcgc cgtcggcgtg
      301 cagttgcttc ctcgcggcgc tgggcgaggg cggcgagcat gtcggcgtac gcctcggcca
      361 cctcccccgc cgtgagcacc accactgtgt cggccgcgtc ggccagcgcc aggacctccc
      421 gcacccgttc gcccacggcc gccgcatcct cgttgccgtc cttgccttcg gcggcccggg
      481 tcgcctcgag gtcgagggcg cggcgggtga ccgcgtgcca tccgtcctcg gtcacggcga
      541 ccccggcccg cagctccccg ccgtcggcgt cggccgccag gagcagatcg aggtcgtcgg
      601 cctcggtgtc gccgccgtcg agcccgagca tctgccgcag gtagcgggtc cattcgatgg
      661 cccggcgtcc ccgggttgcc cgctcgtact cgtgccagcg cgagaggttc cactccagcg
      721 agccgacccc ggcggcgtcg tcctcggtca tgccgccggt caggtccccg atccgtccga
      781 ggagttcgaa cggggcgacg ttcccgccgg tcgccgtctt gaggtcggcg cgggcgagtt
      841 cgagggcggg cgccttcccg tcctgggtct tggcgatgta ctcggcgagg tcgttggcgt
      901 cgcgctcggt ctccagccgc ttgaagtcga cgccgtgccg gtcgtcgggc gtgaaggcgg
      961 ggttgacctt gcgcagggcg gcggtccaca cggaccgcca gtgcccctgc cactcgtcga
     1021 gcgcggcgcc ggtcggctcg aaggtggcga cgatctgctt cgcggaccgc tccccctcgg
     1081 tccggccgcc gaccaggacg atcgcgtgga tgtgcgggtg ccagccgttg atctgcccca
     1141 cggtgacttc ggtcgcgcgg atcatgccga cgtacccgat ccggtctcgg atgccctcgc
     1201 ggtcggcggc ccggtgcccg tccttggccc ggcgtccggc ccacgtgccg cccgtgatca
     1261 gtcgctggta ggcgcccggc cgccgggggc tgtccggcgt cttccgggtg ccctggaggg
     1321 cgtccatgag gtccgcgagc cggtccgtgt gcccatggcg ggccgtgaag gtgaccaggt
     1381 aggcggtccc cccgcgcttg atccactcga ccacggcggc ggtgatctcc tcggcccgct
     1441 tgtgccggat cgtggcggcg cagaccgggc agagccagat ccgcccgcac cgcatcaggc
     1501 ccaggaccac ggacgttccg gccgccgtct gggcgacgat cacgccggag gcagggtcca
     1561 tcagggcgcg gccgcagccc ttgcacgcgg cgtccccgct gatccgccac agcgtccggc
     1621 ggcggctgta ccgggcggct ttccgcagtc gggcagcctc ggtccgcgac gtgcttccta
     1681 cttcccagag gctgtcgcct ctcgggctct ccccatccac cccgtccgga gaaaccgcag
     1741 gtcggagggg tgcgggaaac tctgttgttt ctttcccaag gtgttcgctt ttgcctcggg
     1801 cggcatctcg cgtcacacgc gcgatcgccc gcttcgctgc catccggcag cggtctgagc
     1861 agtagatacg cggccgtttg cccggtgtgt gggcaattgc ggtcccgcag tggcagcggg
     1921 gcccggcggg ccgatctggc aatgcctcgg catcgctccg tactctgggc acgagcaacg
     1981 ttcctgtctc gcccggctaa ggggcgcgag tctgggagcg gacgggtcgg aggtgcgaag
     2041 tccggcccgt tgctctttgg tctggtggga atcctggcac caatcgggcc agaggttccc
     2101 tccgccactc ccgacgcccc ttggggctgg tgtgacttgg agggccgaag agagccccgc
     2161 cgggtatccg gcggggcttt gacgtgcggt cagtgcgtgt gtcggcgagc gatggccacg
     2221 aggccctgga agccgagcgg tccggcgaag tcggcccagt cgcaaccggg ctcagcgcag
     2281 tgggcggacc agccaccgcc gttggggtcc tggaccaggt tcacggtccc ctcggtcagg
     2341 cgtccgtcga agtcggtcat ggtcggtctc ctggtgggtg ggggcggggc gccagcacga
     2401 agtgccggcg ccccgcgggg gttggtcggg tcaggcgccg aaccggcggg cggcggcggc
     2461 gaccaggccg tcggcggcgg ccatggcgcg gtcgcggtcg gtggtgaggg cggtgcggtc
     2521 ggcggcggcc cagtcctgct ctcacgcaac gtctacgtgg acgctcaggg cgacacgatc
     2581 gaggtcgggg agtccgtcgc tccggcgggc cgctggcgcg tccaccggga ctgatcaagg
     2641 cgaatacttc atatgccggc cagcctcgca gagcaggatt cccgttgagc accgccaggt
     2701 gcgaataagg gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc
     2761 tgcccggcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
     2821 gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     2881 gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     2941 agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattaattc gagctcagcc
     3001 aatcgactgg cgagcggcat cgcattcttc gcatcccgcc ctctggcgga tgcaggaaga
     3061 tcaacggatc tcggcccagt tgacccaggg ctgtcgccac aatgtcgcgg gagcggatca
     3121 accgagcaaa ggcatgaccg actggacctt ccttctgaag gctcttctcc ttgagccacc
     3181 tgtccgccaa ggcaaagcgc tcacagcagt ggtcattctc gagataatcg acgcgtacca
     3241 acttgccatc ctgaagaatg gtgcagtgtc tcggcacccc atagggaacc tttgccatca
     3301 actcggcaag atgcagcgtc gtgttggcat cgtgtcccac gccgaggaga agtacctgcc
     3361 catcgagttc atggacacgg gcgaccgggc ttgcaggcga gtgaggtggc aggggcaatg
     3421 gatcagagat gatctgctct gcctgtggcc ccgctgccgc aaaggcaaat ggatgggcgc
     3481 tgcgctttac atttggcagg cgccagaatg tgtcagagac aactccaagg tccggtgtaa
     3541 cgggcgacgt ggcaggatcg aacggctcgt cgtccagacc tgaccacgag ggcatgacga
     3601 gcgtccctcc cggacccagc gcagcacgca gggcctcgat cagtccaagt ggcccatctt
     3661 cgaggggccg gacgctacgg aaggagctgt ggaccagcag cacaccgccg ggggtaaccc
     3721 caaggttgag aagctgaccg atgagctcgg cttttcgcca ttcgtattgc acgacattgc
     3781 actccaccgc tgatgacatc agtcgatcat agcacgatca acggcactgt tgcaaatagt
     3841 cggtggtgat aaacttatca tccccttttg ctgatggagc tgcacatgaa cccattcaaa
     3901 ggccggcatt ttcagcgtga catcattctg tgggccgtac gctggtactg caaatacggc
     3961 atcagttacc gtgagctggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     4021 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     4081 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     4141 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     4201 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4261 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4321 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4381 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4441 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4501 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4561 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4621 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     4681 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     4741 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     4801 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     4861 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     4921 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     4981 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5041 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaagctccat
     5101 ggacccgtgc aggggccccg acacggtcgg ggcccctgca ctctatctat tcacgcgtgg
     5161 taccactagt aagcttggat cctgcagagc tctagatctg aattcgcatg cgcatcgatg
     5221 actcctcgag gtggggggat gagtcagaca tcgtgagtgt ccgtgcccgt acggccgttg
     5281 tgatgatggc aaccgccgat agggccgccc tatccccctc cgtccccacc tgccccgccc
     5341 tgcccggcgg ccccggccgg ggcccctccc gatccgggcc gacgcctcgc gccgtgaagg
     5401 cccgatggtc ctgatcggtc gccattttgt atgtctggtg aaagctgaac ttccgcctta
     5461 ccctcccagg acatggagct tgaggtcagg cacctcaggg cgctgtgcgc catcgccgac
     5521 accggcagcc tgcaccgcgc ggcacgccaa ctgggagtga cacagccctc gttgagcacg
     5581 cagctgcggc gcatcgaaca cgagctgggc ggtgccctgt tcgtccgggc ccgcaccggc
     5641 tgccgcccca caccgctggg ccggctggtg ctcagtcgtg cccgccccct ggtggccgaa
     5701 ttgcgctccc tcgtcagcga ggcccgcgcc gccgccgtcg gcggacgcca gctgcgcgtc
     5761 ggctccacgg ccagccgggc cctggcgggc tggctgcgcc ggctccgccg gcactggcag
     5821 gaacccaccc tgcacatgga cgtctccgcc aacgccctgc tgcgcatggt ggccgacggc
     5881 cacctcgacg tcgccttcgt gcacgaggtc gagggcagcc cgctgcgcgt ccccgaaggg
     5941 ctccgggtcc gcgtactggt ccagcgggaa ccgcagttcg tctgcctgcc ggccgaccac
     6001 ccggccgcgg cgaagccgtc gtacgcctcg ccgacctggc ccacgacccg ctggatgatc
     6061 gaccccaccg tcgacggcga gtgggacgcg gtgcgccggg tcctgcgcgc cgagggactc
     6121 gacccgcgca tcctgcacgg ggactaccac accgccgcgt ccctggtcgc caccggcgag
     6181 gtcgtcaccg tggtccagcc gacctcgccc tcccgcgccg agacggccgt ccgccggctg
     6241 cacggcgacc cgctcggcgt acggctgctg ctggcggccc gcacggacac ggaactggag
     6301 ggcgtctacc ccgacctcgc ggaggcctac ggggaggtcg cccggcaggc cccggcgtac
     6361 cgggagtggc tggaacgcag tgggtcgggg gcactcgtcc cagccctccc gtgacgaccg
     6421 cgtaccccgg cccccttccc gtaacccgac gggagatgcc
//
