hu-beta-MHC-neo-pgk-hygro vector (V009914)

Basic Vector Information

      • Vector Name:
      • hu-beta-MHC-neo-pgk-hygro
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9431 bp
      • Type:
      • Expression vector
      • Source/Author:
      • Zaehres H.

hu-beta-MHC-neo-pgk-hygro vector Vector Map

hu-beta-MHC-neo-pgk-hygro9431 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200Homo sapiens beta myosin heavy chain promoterNeoR/KanRHSV TK poly(A) signalSK primerPGK promoterHygRM13 fwdAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 rev

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

hu-beta-MHC-neo-pgk-hygro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9431 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector hu-beta-MHC-neo-pgk-hygro, complete sequence.
ACCESSION   AY491501
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Expression vectors for selection of cardiomyocytes from 
            differentiating stem cells
  JOURNAL   Patent: DE 10245574-A 27-SEP-2002; CARDION AG; Max-Planck-Str. 15a; 
            Erkrath; Germany;
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2003) CARDION AG, Max-Planck-Str. 15a, Erkrath, 
            NRW 40699, Germany
REFERENCE   3  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9431
                     /organism="Expression vector hu-beta-MHC-neo-pgk-hygro"
                     /mol_type="genomic DNA"
                     /db_xref="taxon:258837"
     regulatory      16..3815
                     /regulatory_class="promoter"
                     /note="Homo sapiens beta myosin heavy chain promoter"
     CDS             3852..4655
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    4662..4710
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4751..4767
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4791..5290
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5308..6333
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDV?GRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     complement(6798..6814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7288..7392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             7393..8253
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8424..9012
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9300..9321
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9336..9366
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9374..9390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9398..9414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"

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