hu-beta-MHC-neo-pgk-hygro vector (V009914)

LOCUS       40924_1354        9431 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector hu-beta-MHC-neo-pgk-hygro, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Expression vectors for selection of cardiomyocytes from 
            differentiating stem cells
  JOURNAL   Patent: DE 10245574-A 27-SEP-2002; CARDION AG; Max-Planck-Str. 15a; 
            Erkrath; Germany;
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2003) CARDION AG, Max-Planck-Str. 15a, Erkrath, 
            NRW 40699, Germany
REFERENCE   3  (bases 1 to 9431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Patent: DE 
            10245574-A 27-SEP-2002; CARDION AG; Max-Planck-Str. 15a; Erkrath; 
            Germany;"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-NOV-2003) CARDION AG, Max-Planck-Str. 15a, Erkrath, NRW 40699, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      16..3815
                     /label=Homo sapiens beta myosin heavy chain promoter
                     /note="Homo sapiens beta myosin heavy chain promoter"
                     /regulatory_class="promoter"
     CDS             3852..4652
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    4662..4710
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4751..4767
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4791..5290
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5308..6330
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    6333..6790
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation
                     signal"
     primer_bind     complement(6798..6814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7288..7392
                     /label=AmpR promoter
     CDS             7393..8250
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8424..9012
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9300..9321
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9336..9366
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9374..9390
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9398..9414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"