LOCUS       40924_1354        9431 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector hu-beta-MHC-neo-pgk-hygro, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Expression vectors for selection of cardiomyocytes from 
            differentiating stem cells
  JOURNAL   Patent: DE 10245574-A 27-SEP-2002; CARDION AG; Max-Planck-Str. 15a; 
            Erkrath; Germany;
REFERENCE   2  (bases 1 to 9431)
  AUTHORS   Zaehres H.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-NOV-2003) CARDION AG, Max-Planck-Str. 15a, Erkrath, 
            NRW 40699, Germany
REFERENCE   3  (bases 1 to 9431)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9431)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Patent: DE 
            10245574-A 27-SEP-2002; CARDION AG; Max-Planck-Str. 15a; Erkrath; 
            Germany;"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (30-NOV-2003) CARDION AG, Max-Planck-Str. 15a, Erkrath, NRW 40699, 
            Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      16..3815
                     /label=Homo sapiens beta myosin heavy chain promoter
                     /note="Homo sapiens beta myosin heavy chain promoter"
                     /regulatory_class="promoter"
     CDS             3852..4652
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     polyA_signal    4662..4710
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     4751..4767
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4791..5290
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             5308..6330
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     polyA_signal    6333..6790
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation
                     signal"
     primer_bind     complement(6798..6814)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7288..7392
                     /label=AmpR promoter
     CDS             7393..8250
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      8424..9012
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    9300..9321
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9336..9366
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9374..9390
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9398..9414
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tcgagggccc tcgaggatct atctggtcca gaccatcctt tgaaccaagt ggacttagtg
       61 gggctgaagg aggtgtgggg acaaagaacc tatggcaggg caaccggccc tcctgtctgc
      121 agcctggctt cctcaatttt gtgttcctaa cctaagagct gccattcagt gagggcccac
      181 agtgtgtctg ctgtgtgtag actctgcttg tgcaatctct aatggcctga gtagccctgg
      241 aaacaaaatg tggctatccc catgttacag atgacaaaac tggctcaaag caattatatg
      301 actgacgtag accacacagc tagaaaatgg aatcactggg tttcaaagtg gatttttgtg
      361 actgactcca aagctgacca gagtgctcat cctgtatgct tctgaggcct ctggccctcc
      421 agaccggtca ctccccacaa agtctccctt ttggtgagtt gtgtccctcc aaacactccc
      481 tgaaggcaag atcaacctgc cctaactacc ccaaagtgaa ctaggccagc cccaagcctg
      541 tctccggccc tccatctctc tctttgttca catccatcag aaagcagaga cctagagtag
      601 ggaatgagag ggggtgcaca agtacaaaag attttgaagc agcaggaaga cgaaagaagt
      661 acaagaagga gcagctgttt gtaaaaggga aaatctgaga caggcagaaa gctcagctcc
      721 ctaatcctag gctcgtgtgg agccagggca gggtccccca aggtcaggga ttctgggggc
      781 aggcggatgg gagagtcagc ctccactcct gggtggctcc cctctgccct gtgcacaaac
      841 aacgagtgcc tcattcctcc cctgcaccaa gtgccctgcc tcttcctgcc ccgtttcttc
      901 cttccctttg ggtaccggat cttttttctg attcccctct ctccatgctg acatcgcttc
      961 taattcgccc tggtgtctcc ctccctcaca attactggga gtaattttag caacttttcc
     1021 tctgaactac cgctggcgtg ttcacagcaa atggcccctg ttttggcttg acacttgatc
     1081 agggtggtga cagagtgcag acaaagccgg cacaatgcgg ggaggggagg accggggcag
     1141 atcaccttcc tttcacttca gggccttatt tggtctacct tgccgaagcg gccctccttc
     1201 cagcctctct ttatttatag cattttgctt gctctccttt atcttgctat cacttgtagc
     1261 acactctgca caatccccac ccaccccagc atggtggggc ccctggctca ctctccccac
     1321 aaggaatgtg catgtgtgca aagggtaggc ctggatgcca tttctacaaa cagttgacta
     1381 aagcagtcca gagcagggat ctttctttgg gtcattgtta tgtccttatt gaccagaata
     1441 atgcctggca catagtaggc actcaataaa tacttgttga atggaggaat ctgcaaagct
     1501 gctcagtctt cagacagcag caagcagcag gaagagaatc atgatgtaac caagagcagg
     1561 tgctggagga gctagggagt gagtggggat ctttaaggac agcaggccct ctgatctgtc
     1621 tgagcgtgtg agcaaagcct agttagggag gtgagttcta tctgcaatga gagggggcat
     1681 gggtggagac agataacacc aacaccaaca gaccagacct cccaaacccg aaaggagaga
     1741 caaagtgtga ttgatttccc aaatgcagaa aagactaccc aagtccccag ccaagaagaa
     1801 tcgctggcct gtggatgaag acactggctt tgaaatggct caactacgtc ccaagcaccc
     1861 tttgtgcatc ttaattgccc tgggaaatca gggaggggtg gcagtttgga gcttggaaag
     1921 ctggaaggtg gggacgaaag gagtttttgc ccctcatcgc caatccttga ggttcctaca
     1981 gggaacctca gacaacagct gtcttagggg tttccttcca gctccaagca gctgtattat
     2041 ctatagcttt gagagggtct gtctctgtca atggggctga ttcctcagca cctggcaggg
     2101 cctggggggg actgccagag tctgttctgc cgggcatgtt agtagcacag actactggga
     2161 taacctcagc aactgtatac cttagcactg cagagctgcg gtgttgtcag acacaggccc
     2221 ttgcagttcg tggtctaccc ccacttgcct gcacccccct tatagctgct caatgtcctc
     2281 agactgtacc ttagtgcctt ggttaccaag caacccccac agaggccctc ctcctggatt
     2341 ttcccttccc tgctatgccc accccacctt caaaatgtac ctgctgcacc catcccccag
     2401 gcctgcctca ctccttccaa gctgacagct aatcagatgg actaatcgca tgcgcggaag
     2461 agctgtgctc gggaagaagg gggagatttc acaccaaatc gtgcagtggt tgcctagaca
     2521 cggagccagt caagcttgca tgccccgttg ctagaagagc atggcatcgg ttgtgttctg
     2581 tgctcgtagg ctgggcccca gctccctttc ctcccctcct ttgtggctgt tcctcaggcc
     2641 tgcagagggg aagatttttc ctgtcctgtt actctgcctc acttgccaga acactagtcg
     2701 cctccagctc tggataaagc tgaggctggg tgggccagac aacagctgtc ttaggggtct
     2761 ccttccagct ccaagtagtt gtattatcta tagctctgag agggtctgtc tctgggctca
     2821 ggcctttagt acctgtgggg cagagccagg aacgtgagtc aggcaaaccg ctccaaacta
     2881 ggaccaatgc agctgggtcc tgctctctgc gcataggggg tccccacatc tttggaatcc
     2941 cagcccacct ttccaggcta ccctccacag gccgggctcc actcccatct gctgaggttt
     3001 cccaccttga atcctgctgc cccgattagc tgtataacct taaagaatgc actgtccctc
     3061 tccattaaat gaagtgcttg gatggattgc taaaggcctg tctggctcgg aggcttggtg
     3121 cctcaacaca ttgcctgctg gtccaaggaa atcagtgcct gagccagagt ccccatctct
     3181 aagctccatg gttattgttc ttgccacctg gctaggaaat gtccttccag ctgccccagt
     3241 ctagctgcct caccctgggg ccatgcccca actctgtcct acccttctct gctgctgaca
     3301 ctcagcccct tcccagcttc cagttggata caggacctgg gccaggagag cagggaggac
     3361 actgtggaaa tgcggccagg ccatcagggg cctcgcagca ggggactgga gggggagcag
     3421 tgtccagggc cagaagtgcc ctgcgggaga gccaggacat tggctgcctg tggtcttggt
     3481 ggtcgtggtc agttccctct cctgccagct gtggaatgtg aggcctggcc tgggagatat
     3541 ttttgctgca ctttgagcca ccccgccccc tggaactcag accctgcaca gtccatgcca
     3601 taacaatgac gaccacttcc aattgtttcc tagctggaga ggcggggagg ggagcactgt
     3661 ttgggaaggg ggggagcctg ggggaaatgc ttctagtgac aacagccctt tctaaatccg
     3721 gctagggact gggtgccgtt gggggtgggg gtgccctgct gccccatata tacagcccct
     3781 gagaccaggt ctggctccac agctctgtcc tgctccttaa ggcggccgca ccgagcgacc
     3841 ctgcagccaa tatgggatcg gccattgaac aagatggatt gcacgcaggt tctccggccg
     3901 cttgggtgga gaggctattc ggctatgact gggcacaaca gacaatcggc tgctctgatg
     3961 ccgccgtgtt ccggctgtca gcgcaggggc gcccggttct ttttgtcaag accgacctgt
     4021 ccggtgccct gaatgaactg caggacgagg cagcgcggct atcgtggctg gccacgacgg
     4081 gcgttccttg cgcagctgtg ctcgacgttg tcactgaagc gggaagggac tggctgctat
     4141 tgggcgaagt gccggggcag gatctcctgt catctcacct tgctcctgcc gagaaagtat
     4201 ccatcatggc tgatgcaatg cggcggctgc atacgcttga tccggctacc tgcccattcg
     4261 accaccaagc gaaacatcgc atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg
     4321 atcaggatga tctggacgaa gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc
     4381 tcaaggcgcg catgcccgac ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc
     4441 cgaatatcat ggtggaaaat ggccgctttt ctggattcat cgactgtggc cggctgggtg
     4501 tggcggaccg ctatcaggac atagcgttgg ctacccgtga tattgctgaa gagcttggcg
     4561 gcgaatgggc tgaccgcttc ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca
     4621 tcgccttcta tcgccttctt gacgagttct tctgagggga tcggcaataa aaagacagaa
     4681 taaaacgcac gggtgttggg tcgtttgttc ggatccgtcg actctagagc tccaccgcgg
     4741 tggcggccgc tctagaacta gtggatcccc cgggctgcag gaattctacc gggtagggga
     4801 ggcgcttttc ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg
     4861 ctacacaatg tgcctctggc ctcgcacaca ttccacagcc accggtaggc gccaaccggc
     4921 tccgttctta ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc
     4981 ccccgccccg cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag
     5041 tctcgtgcag atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg
     5101 ccaatagcag ctttgctcct tcgctttctg ggcttcagag gctgggaagg ggtgggtccg
     5161 ggggcgggct caggggcggg ctcaggggcg gtcgggcgcc cgaaggtcct ccggaggccc
     5221 ggcattctgc acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc
     5281 gggcctttcg acctgcatcc cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc
     5341 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     5401 gaagaatctc gtgctttcag cttcgatgta vgagggcgtg gatatgtcct gcgggtaaat
     5461 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     5521 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     5581 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     5641 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     5701 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     5761 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     5821 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     5881 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     5941 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     6001 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     6061 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     6121 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     6181 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     6241 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     6301 cgccccagca ctcgtccgag ggcaaaggaa tagagtatgc agaaattgat gatctattaa
     6361 acaataaaga tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga
     6421 gaacagagta cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg
     6481 gattagataa atgcctgctc tttactgaag gctctttact attgctttat gataatgttt
     6541 catagttgga tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc
     6601 cactcatgat ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc
     6661 actttgtggt tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga
     6721 gatcagcagc ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat
     6781 tccatcagaa gcttggcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     6841 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     6901 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg
     6961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc
     7021 agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct
     7081 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     7141 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     7201 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     7261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9181 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     9241 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     9301 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     9361 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     9421 tacgaattcc c
//
