Basic Vector Information
- Vector Name:
- Hsp70_LexA-HP1
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9422 bp
- Type:
- Cloning vector
- Replication origin:
- ori
- Source/Author:
- Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN.
- Promoter:
- hsp70
Hsp70_LexA-HP1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
Hsp70_LexA-HP1 vector Sequence
LOCUS 40924_1349 9422 bp DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector Hsp70_LexA-HP1, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9422)
AUTHORS Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
Belyaeva ES, Belyakin SN.
TITLE Repressive histone marks renewal impedes replication fork
progression and contributes to late replication in Drosophila
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 9422)
AUTHORS Belyakin SN.
TITLE Direct Submission
JOURNAL Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
8/2, Novosibirsk, NSO 630090, Russia
REFERENCE 3 (bases 1 to 9422)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 9422)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
Novosibirsk, NSO 630090, Russia"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9422
/mol_type="other DNA"
/organism="synthetic DNA construct"
protein_bind 220..289
/label=attB
/note="attB site for the phi-C31 integrase (Groth et al.,
2000)"
protein_bind 534..567
/label=FRT (minimal)
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
promoter 1448..1900
/label=hsp70 promoter
/note="Drosophila melanogaster hsp70Bb promoter"
primer_bind complement(1970..1986)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 2023..2628
/label=LexA
/note="E. coli DNA-binding protein that represses genes
involved in the SOS response to DNA damage"
CDS 2638..2658
/label=SV40 NLS
/note="nuclear localization signal of SV40 (simian virus
40) large T antigen"
CDS 2665..3279
/gene="Su(var)205"
/label=Su(var)205
/note="Heterochromatin protein 1 from Drosophila
melanogaster. Accession#: P05205"
protein_bind 7501..7534
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
rep_origin complement(7603..8191)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8365..9222)
/label=AmpR
/note="beta-lactamase"
promoter complement(9223..9327)
/label=AmpR promoter
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