Hsp70_LexA-HP1 vector (V009915)

Basic Vector Information

Vector Name:
Hsp70_LexA-HP1
Antibiotic Resistance:
Ampicillin
Length:
9422 bp
Type:
Cloning vector
Replication origin:
ori
Source/Author:
Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN, Belyaeva ES, Belyakin SN.
Promoter:
hsp70

Hsp70_LexA-HP1 vector Vector Map

Hsp70_LexA-HP19422 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200attBFRT (minimal)hsp70 promoterKS primerLexASV40 NLSHeterochromatin protein 1FRT (minimal)oriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

Hsp70_LexA-HP1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V009915                 9422 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009915
VERSION     V009915
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 9422)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    220..289
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    534..567
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        1448..1900
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1970..1986)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2023..2628
                     /label="LexA"
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             2638..2658
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2665..3279
                     /gene="Su(var)205"
                     /label="Heterochromatin protein 1"
                     /note="Heterochromatin protein 1 from Drosophila
                     melanogaster. Accession#: P05205"
     protein_bind    7501..7534
                     /label="FRT (minimal)"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(7603..8191)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8365..9222)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9223..9327)
                     /label="AmpR promoter"

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