LOCUS       V009915                 9422 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009915
VERSION     V009915
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9422)
  AUTHORS   Posukh OV, Maksimov DA, Koryakov DE, Pokholkova GV, Skvortsova KN,
            Belyaeva ES, Belyakin SN.
  TITLE     Repressive histone marks renewal impedes replication fork
            progression and contributes to late replication in Drosophila
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9422)
  AUTHORS   Belyakin SN.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave.
            8/2, Novosibirsk, NSO 630090, Russia
REFERENCE   3  (bases 1 to 9422)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9422)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (15-OCT-2015) Genomics Lab, IMCB SD RAS, Lavrentjeva Ave. 8/2,
            Novosibirsk, NSO 630090, Russia"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9422
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    220..289
                     /label="attB"
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     protein_bind    534..567
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     promoter        1448..1900
                     /label="hsp70 promoter"
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     complement(1970..1986)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2023..2628
                     /label="LexA"
                     /note="E. coli DNA-binding protein that represses genes
                     involved in the SOS response to DNA damage"
     CDS             2638..2658
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             2665..3279
                     /gene="Su(var)205"
                     /label="Heterochromatin protein 1"
                     /note="Heterochromatin protein 1 from Drosophila
                     melanogaster. Accession#: P05205"
     protein_bind    7501..7534
                     /label="FRT (minimal)"
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(7603..8191)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(8365..9222)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(9223..9327)
                     /label="AmpR promoter"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataggtc tagaactagt cgacgatgta ggtcacggtc tcgaagccgc ggtgcgggtg
      241 ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct ggtccatcat
      301 gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca ccgggaagcc
      361 ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg cgacggcgtc
      421 ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg cgggcatgtc
      481 gacaagccga attgatccac tagaaggcca gatctgcggc caattccctg catgaagttc
      541 ctattctcta gaaagtatag gaacttcggc gcgcctgcag gatcgatgga tccagatctg
      601 ctagcggccg cctagaacta gtggatcccc cgggctgcag gaattcggcg cgccgatgcg
      661 gccgcacaaa gtcaatgggg aacatactac gtactaaaac ttttaaaatt ttaattaaat
      721 aatttaaaat tttaggatta aacttcgatt tagtgttttg tgtaaagagt ttcttgacag
      781 tctcaaaagt gtgtaaactt ggaatttgtg gtttattttc ttgtataatt aatattttta
      841 atttattttt tgatttataa gtaaaataaa tattgtttaa ttatattata taaaaaaatt
      901 gcgtttaatt aagccaaaaa cccttaattt ttagctttaa agtcaaaaat tcaacttatt
      961 tcacagtgtg taaacagttt cttgacaaac tgtatatcta atatttttaa tttttttttt
     1021 atttataagt aaaataaata ttgtttaatt atattaaaaa aaaaattccg tttaattaag
     1081 caaataaccc ttaattttta cctttaaagt caaaaattca acatatttca cagtttgtaa
     1141 acttgataaa ctgtatgtaa tagtcttggc tttcctcata tgtacatatg tatgtaaata
     1201 tgtaaaataa gtcgcaacta aattctaata cacttctcag tcttaaatta attttatcgt
     1261 atattaaaac agaagaaagt ccgttaatcg ttgatttcgt taactaaaag tacaaaataa
     1321 tctttaatct ttagaaacgc agcaatgttt tggcggcata cgcataacaa agcgcttcga
     1381 ttatctttat cataagttat ttaagcagcc gtatttataa agaaatttcc aaaataaagc
     1441 gaatattcta gaatcccaaa acaaactggt tattgtggta ggtcatttgt ttggcagaaa
     1501 gaaaactcga gaaatttctc tggccgttat tcgttattgt ctcttttctt tttgggtctc
     1561 tccctctctg cactaatgct ctctcactct gtcacacagt aaacgacata ctgctctcgt
     1621 tggttcgaga gagcgcgcct cgaatgttcg cgaaaagagc gccggagtat aaatagaggc
     1681 gcttcgtcta cggagcgaca attcaattca aacaagcaaa gtgaacacgt cgctaagcga
     1741 aagctaagca aataaacaag cgcagctgaa caagctaaac aatctgcagt aaagtgcaag
     1801 ttaaagtgaa tcaattataa gtaaccagca accaagtaaa tcaactgcaa ctactgaaat
     1861 ctgccaagaa gtaattattg aataccagaa gagaactctg aatactttca acaagttacc
     1921 gagaaagaag aactcacaca caatccagat cttttaatta aaagcttatc gataccgtcg
     1981 acctcgaggg ggggcccggt acctgaatta attccgggcg gaatgaaagc gttaacggcc
     2041 aggcaacaag aggtgtttga tctcatccgt gatcacatca gccagacagg tatgccgccg
     2101 acgcgtgcgg aaatcgcgca gcgtttgggg ttccgttccc caaacgcggc tgaagaacat
     2161 ctgaaggcgc tggcacgcaa aggcgttatt gaaattgttt ccggcgcatc acgcgggatt
     2221 cgtctgttgc aggaagagga agaagggttg ccgctggtag gtcgtgtggc tgccggtgaa
     2281 ccacttctgg cgcaacagca tattgaaggt cattatcagg tcgatccttc cttattcaag
     2341 ccgaatgctg atttcctgct gcgcgtcagc gggatgtcga tgaaagatat cggcattatg
     2401 gatggtgact tgctggcagt gcataaaact caggatgtac gtaacggtca ggtcgttgtc
     2461 gcacgtattg atgacgaggt taccgttaag cgcctgaaaa aacagggcaa taaagtcgaa
     2521 ctgttgccag aaaatagcga gtttaaacca attgtcgtag atcttcgtca gcagagcttc
     2581 accattgaag ggctggcggt tggggttatt cgcaacggcg actggctgga attggccccc
     2641 aagaaaaaga gaaaggtgga attcggcacg aggcgcgaca accctgagag ctcggcaaag
     2701 gtttccgatg ccgaagagga ggaggaggag tacgccgtgg aaaagatcat cgacaggcgg
     2761 gtgcgcaagg gaaaggtgga gtactatctg aaatggaagg gctatcccga aactgagaac
     2821 acgtgggagc cggagaacaa tctcgactgc caggatctta tccagcagta cgaggcgagc
     2881 cgcaaggatg aggagaagtc agccgcctcc aagaaggatc gacccagcag cagcgccaag
     2941 gccaaggaaa ctcaaggacg cgccagcagt tcgacgtcca cagcaagcaa gcgaaagtcc
     3001 gaagaaccaa cagcgccctc tggcaataaa tcaaaacgta ccacagatgc ggagcaggac
     3061 accattcccg tttcaggatc taccggattc gatcgcggcc tggaggccga aaagatcttg
     3121 ggtgcctccg acaataatgg ccgcctgaca ttcctcattc agttcaaagg cgtggaccaa
     3181 gcagaaatgg tgccctcctc agtggccaat gaaaaaattc cacgaatggt aatccacttc
     3241 tacgaagagc gcctatcctg gtactctgat aatgaagatt aaggtaccga attctagtat
     3301 gtatgtaagt taataaaacc catttttgcg gaaagtagat aaaaaaaaca tttttttttt
     3361 ttactgcact ggatatcatt gaacttatct gatcagtttt aaatttactt cgatccaagg
     3421 gtatttgatg taccaggttc tttcgattac ctctcactca aaatgacatt ccactcaaag
     3481 tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg
     3541 tccgctatct ctttcgccac cgtttgtagc gttacgtagc gtcaatgtcc gccttcagtt
     3601 gcattttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca attaaacaca
     3661 aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga gtgattgggg
     3721 tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg caatgggcca
     3781 agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg agcatctgaa
     3841 caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg aactgcgatt
     3901 gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca aaccgcaatc
     3961 gcacaaacaa atagtgacac gaaacagatt attctggtag ctgtgctcgc tatataagac
     4021 aatttttaag atcatatcat gatcaagaca tctaaaggca ttcattttcg actacattct
     4081 tttttacaaa aaatataaca accagatatt ttaagctatg cacaaaaaat aaataaaagt
     4141 ataaacctac ttcgtaggat acttcgtttt gttcggggtt agatgagcat aacgcttgta
     4201 gttgatattt gagatcccct atcattgcag ggtgacagcg gacgcttcgc agagctgcat
     4261 taaccagggc ttcgggcagg ccaaaaacta cggcacgctc ctgccaccca gtccgccgga
     4321 ggactccggt tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat
     4381 ggacatcttt ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac
     4441 acgcggacta ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa
     4501 cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta
     4561 ccaatttgaa actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc
     4621 agttccggtg ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc
     4681 atccaagtat cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag
     4741 atgcaggcca ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc
     4801 agggaacacc tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag
     4861 cgagtggccc gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg
     4921 atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca
     4981 ttcgcctccg aggcactaac cgatccgccg cttctgatct gcgatgagcc cacctccgga
     5041 ctggactcat ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc
     5101 aagaccgtca tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag
     5161 atccttctga tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac
     5221 ttcttttcct agtgagttcg atgtgtttat taagggtatc tagcattaca ttacatctca
     5281 actcctatcc agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt
     5341 acaggtgttg gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat
     5401 atggcgacaa ttttgccatt agcaaagtag cccgggatat ggagcagttg ttggccacca
     5461 aaaatctgga gaagccactg gagcagccgg agaatgggta cacctacaag gccacctggt
     5521 tcatgcagtt ccgggcggtc ctgtggcgat cctggctgtc ggtgctcaag gaaccactcc
     5581 tcgtaaaagt gcgacttatt cagacaacgg tgagtggttc cagtggaaac aaatgatata
     5641 acgcttacaa ttcttggaaa caaattcgct agattttaga tagaattgcc tgattccaca
     5701 cccttcttag tttttttcaa tgagatgtat agtttatagt tttgcagaag ataaataaat
     5761 ttcatttaac tcgcgaatat taatgagatg cgagtaacat tttaatttgc agatggttgc
     5821 catcttgatt ggcctcatct ttttgggcca acaactcacg caagtgggtg tgatgaatat
     5881 caacggagcc atcttcctct tcctgaccaa catgaccttt caaaacgtct ttgccacgat
     5941 aaatgtaagt catgtttaga atacatttgc atttcaataa tttactaact ttctaatgaa
     6001 tcgattcgat ttaggtgttc acctcagagc tgccagtttt tatgagggag gcccgaagtc
     6061 gactttatcg ctgtgacaca tactttctgg gcaaaacgat tgccgaattg ccgctttttc
     6121 tcacagtgcc actggtcttc acggcgattg cctatccgat gatcggactg cgggccggag
     6181 tgctgcactt cttcaactgc ctggcgctgg tcactctggt ggccaatgtg tcaacgtcct
     6241 tcggatatct aatatcctgc gccagctcct cgacctcgat ggcgctgtct gtgggtccgc
     6301 cggttatcat accattcctg ctctttggcg gcttcttctt gaactcgggc tcggtgccag
     6361 tatacctcaa atggttgtcg tacctctcat ggttccgtta cgccaacgag ggtctgctga
     6421 ttaaccaatg ggcggacgtg gagccgggcg aaattagctg cacatcgtcg aacaccacgt
     6481 gccccagttc gggcaaggtc atcctggaga cgcttaactt ctccgccgcc gatctgccgc
     6541 tggactacgt gggtctggcc attctcatcg tgagcttccg ggtgctcgca tatctggctc
     6601 taagacttcg ggcccgacgc aaggagtagc cgacatatat ccgaaataac tgcttgtttt
     6661 ttttttttac cattattacc atcgtgttta ctgtttattg ccccctcaaa aagctaatgt
     6721 aattatattt gtgccaataa aaacaagata tgacctatag aatacaagta tttccccttc
     6781 gaacatcccc acaagtagac tttggatttg tcttctaacc aaaagactta cacacctgca
     6841 taccttacat caaaaactcg tttatcgcta cataaaacac cgggatatat tttttatata
     6901 catacttttc aaatcgcgcg ccctcttcat aattcacctc caccacacca cgtttcgtag
     6961 ttgctctttc gctgtctccc acccgctctc cgcaacacat tcaccttttg ttcgacgacc
     7021 ttggagcgac tgtcgttagt tccgcgcgat tcggttcgct caaatggttc cgagtggttc
     7081 atttcgtctc aatagaaatt agtaataaat atttgtatgt acaatttatt tgctccaata
     7141 tatttgtata tatttccctc acagctatat ttattctaat ttaatattat gactttttaa
     7201 ggtaattttt tgtgacctgt tcggagtgat tagcgttaca atttgaactg aaagtgacat
     7261 ccagtgtttg ttccttgtgt agatgcaacc aaactgtctc acgacgtttt gtgcatacac
     7321 attattttgt gtgtatatgg tgacgtcaca actacaagca ataagaaatt ttccagacgt
     7381 ggctttgttt aagcaaccta ctctatttca gctaaaaata agtggatttc gttggtaaaa
     7441 tacttcaatt aactaaacaa ctaactaact aataacatgc acacaaatgc tcgagtcgac
     7501 gaagttccta ttctctagaa agtataggaa cttcggatcc gccatgtgag caaaaggcca
     7561 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     7621 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7681 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7741 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7801 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7861 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7921 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7981 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     8041 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     8101 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     8161 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     8221 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     8281 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     8341 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     8401 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8461 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8521 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8581 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8641 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8701 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8761 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8821 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8881 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8941 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     9001 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     9061 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     9121 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     9181 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     9241 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     9301 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     9361 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9421 tc
//
