Basic Vector Information
HSP18.2-LUC-NOS-At5g59720 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
HSP18.2-LUC-NOS-At5g59720 vector Sequence
LOCUS 40924_1344 5452 bp DNA circular SYN 17-DEC-2018
DEFINITION Control vector HSP18.2-LUC-NOS-At5g59720, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5452)
AUTHORS Kovtun Y, Chiu WL, Tena G, Sheen J.
TITLE Functional analysis of oxidative stress-activated mitogen-activated
protein kinase cascade in plants
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
PUBMED 10717008
REFERENCE 2 (bases 1 to 5452)
AUTHORS Sheen J.
TITLE Direct Submission
JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General
Hospital, 185 Cambridge St. Simches Research Building-CPZN7600,
Boston, MA 02114, USA
REFERENCE 3 (bases 1 to 5452)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 5452)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages:
"2940-2945"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114,
USA"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5452
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind complement(15..31)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(39..55)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(63..93)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(108..129)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(417..1005)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1179..2036)
/label=AmpR
/note="beta-lactamase"
promoter complement(2037..2141)
/label=AmpR promoter
primer_bind 2615..2631
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 2652..2668
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
regulatory 2670..3388
/label=Hsp18.2 enhancer/promoter
/note="Hsp18.2 enhancer/promoter"
/regulatory_class="enhancer"
CDS 3389..5038
/label=luciferase
/note="firefly luciferase"
terminator 5196..5447
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
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