LOCUS       40924_1344        5452 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector HSP18.2-LUC-NOS-At5g59720, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 5452)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5452)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: 
            "2940-2945"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5452
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2652..2668
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2670..3388
                     /label=Hsp18.2 enhancer/promoter
                     /note="Hsp18.2 enhancer/promoter"
                     /regulatory_class="enhancer"
     CDS             3389..5038
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      5196..5447
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctg
     2641 gtggcggccg ctctagaact agtggatccc ccgtcatttc ttctggttca agcatgacat
     2701 gaacaggcaa taaataagtt gagattttga tcacagtaac tgatacttga gtcgaatcat
     2761 ttagattttt ttttttttag tttacttgtt tagtaaatat tttgtccatg tttgtcacaa
     2821 aaacgtggct cagttcttgt atatatggag acaaaaaaat ccattaaaag attgttgaca
     2881 ttctcggaaa tttagtgcca actgttattg cgagaactta ctatagtttt cctttggcga
     2941 aaagctaata atcttaaatc ttgattttgt cctcttttct ctgagttaga ttttcttaaa
     3001 ttccacttcc gacctattaa gaaatgggct tttgcaaaga agatccgctt cactgagccc
     3061 gtatctcgaa gaggataata caacaacaaa gcaaaacggc acgtagtttt aattgtaacc
     3121 aaggattgca tttcggtctt gtttcaacaa acgaaacttc ctgaaatgcc aagaaaaatc
     3181 tggtcatttc accacagtga tcattgtgta tgtgttctaa agactccaag cgaaggtttt
     3241 agaaaaagga gcattttcta ttctattcaa gaaactcgaa gaacattctc tcttcatcct
     3301 ctaacttccc tataaatatg tcctttgcta atcagatcaa atcagcagga aaatcaagaa
     3361 ccaaaagtct cccgaaaagc aacgaaccat ggaagacgcc aaaaacataa agaaaggccc
     3421 ggcgccattc tatcctctag aggatggaac cgctggagag caactgcata aggctatgaa
     3481 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtgaacat
     3541 cacgtacgcg gaatacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
     3601 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
     3661 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
     3721 acgtgaattg ctcaacagta tgaacatttc gcagcctacc gtagtgtttg tttccaaaaa
     3781 ggggttgcaa aaaattttga acgtgcaaaa aaaattacca ataatccaga aaattattat
     3841 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
     3901 tctacctccc ggttttaatg aatacgattt tgtaccagag tcctttgatc gtgacaaaac
     3961 aattgcactg ataatgaatt cctctggatc tactgggtta cctaagggtg tggcccttcc
     4021 gcatagaact gcctgcgtca gattctcgca tgccagagat cctatttttg gcaatcaaat
     4081 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
     4141 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
     4201 gctgttttta cgatcccttc aggattacaa aattcaaagt gcgttgctag taccaaccct
     4261 attttcattc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
     4321 aattgcttct gggggcgcac ctctttcgaa agaagtcggg gaagcggttg caaaacgctt
     4381 ccatcttcca gggatacgac aaggatatgg gctcactgag actacatcag ctattctgat
     4441 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
     4501 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcagagag gcgaattatg
     4561 tgtcagagga cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
     4621 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
     4681 cttcttcata gttgaccgct tgaagtcttt aattaaatac aaaggatatc aggtggcccc
     4741 cgctgaattg gaatcgatat tgttacaaca ccccaacatc ttcgacgcgg gcgtggcagg
     4801 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
     4861 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
     4921 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
     4981 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaagt ccaaattgta
     5041 aaatgtaact gtattcagcg atgacgaaat tcttagctat tgtaatatta tatgcaaatt
     5101 gatgaatggt aattttgtaa ttgtgggtca ctgtactatt ttaacgaata ataaaatcag
     5161 gtataggtaa ctaaaaagga attcgagctc gaattgatcg ttcaaacatt tggcaataaa
     5221 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
     5281 ttacgttaag catgtaataa ttaacatgta atgcatgacg ttatttatga gatgggtttt
     5341 tatgattaga gtcccgcaat tatacattta atacgcgata gaaaacaaaa tatagcgcgc
     5401 aaactaggat aaattatcgc gcgcggtgtc atctatgtta ctagatctct ag
//
