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Basic Vector Information
Basic Vector Information | |||
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Vector Name | HO-poly-KanMX4-HO | Antibiotic Resistance | Ampicillin |
Length | 6062 bp | Type | Cloning vector |
Source | Voth WP, Richards JD, Shaw JM, Stillman DJ. |
HO-poly-KanMX4-HO vector Vector Map
HO-poly-KanMX4-HO vector Sequence
LOCUS Exported 6062 bp ds-DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector HO-poly-KanMX4-HO, complete sequence. ACCESSION AF324728 VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6062) AUTHORS Voth WP, Richards JD, Shaw JM, Stillman DJ. TITLE Yeast vectors for integration at the HO locus JOURNAL Nucleic Acids Res. 29 (12), E59 (2001) PUBMED 11410682 REFERENCE 2 (bases 1 to 6062) AUTHORS Voth WP, Richards J, Shaw J, Stillman DJ. TITLE Direct Submission JOURNAL Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 6062) AUTHORS . TITLE Direct Submission JOURNAL Exported Dec 18, 2018 from SnapGene 4.1.8 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6062 /organism="Cloning vector HO-poly-KanMX4-HO" /mol_type="genomic DNA" /db_xref="taxon:153593" misc_feature 1..6062 /note="vector designed for targeted integration to the HO locus in yeast; plasmid contains two fragments from the HO promoter inserted in the pUC21-NotI polylinker, along with the KanMX selectable marker; HO region is bracketed by two NotI sites, with polylinker sites between the HO and KanMX4 regions" gene 1069..2425 /label=kanMX /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 1069..1412 /label=TEF promoter /note="Ashbya gossypii TEF promoter" CDS 1413..2222 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 2228..2425 /label=TEF terminator /note="Ashbya gossypii TEF terminator" primer_bind 2995..3011 /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(3026..3044) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3051..3067) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 3939..4043 /gene="bla" /label=AmpR promoter CDS 4044..4904 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 5075..5663 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 5951..5972 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." protein_bind 6027..6043 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6051..5 /label=M13 rev /note="common sequencing primer, one of multiple similar variants"