LOCUS       40924_1329        6062 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector HO-poly-KanMX4-HO, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Voth WP, Richards JD, Shaw JM, Stillman DJ.
  TITLE     Yeast vectors for integration at the HO locus
  JOURNAL   Nucleic Acids Res. 29 (12), E59 (2001)
  PUBMED    11410682
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Voth WP, Richards J, Shaw J, Stillman DJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical
            Drive, 5C124 SOM, Salt Lake City, UT 84132, USA
REFERENCE   3  (bases 1 to 6062)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6062)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2001"; volume: "29"; issue: "12"; pages: "E59"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 
            5C124 SOM, Salt Lake City, UT 84132, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6062
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     gene            1069..2425
                     /label=kanMX
                     /note="yeast selectable marker conferring kanamycin
                     resistance (Wach et al., 1994)"
     primer_bind     2995..3011
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(3026..3044)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3051..3067)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        3939..4043
                     /label=AmpR promoter
     CDS             4044..4901
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5075..5663
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5951..5972
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     protein_bind    6027..6043
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc
       61 cagctgaagc ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat
      121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta
      181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaatagaaaa
      241 catatgctat aaggttgata ttctcacgag taagcggcac ttgctactta ttgacattgc
      301 agatttttgg ctacagaaat agtatattag agattataat tgctaatcaa atcaaaatat
      361 aaaattagta aaccaaacca tttataccct tccttagtag ttatggattg ttttttaatg
      421 atatttctgc aaaccaaaga aagattgtta tccagataga atttagtttt gatattcatt
      481 tttttgttga agattgaacg ccatatctgg gcctcataat tcaaaagacg gtgccattat
      541 cggtagcgtt tcgcattgta ctggatttca gaaatttcac agttgatgaa tcgaaaagaa
      601 tggtctcatt gcaacacgta aggttaagat gtcccttttt accattatag gcaataaatg
      661 aatcataaaa cgaccgtata ctggtgaaat agtagggaga acgagtacct gtagtaaaaa
      721 gtataaatca tagttaatcg ggcaatgtcc ctcgatcaag gagtattgtg tcatgttcga
      781 gacaaacgcc aacatttttg tttcttttgg acaaatgttg tttgcattta tgatccgtta
      841 tattttgatc taatgtagag ttgcacgtag ttcttactgg caaagaaatc gatgcatacc
      901 aaaaaagaat aaaggtgata tttgatcttt accgtttagt tccaacgtaa aattgtgcct
      961 ttggacttaa aatggcgtcg tacgctgcag gtcgacggat ccccgggtta attaaggcgc
     1021 gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
     1081 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
     1141 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
     1201 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcaga cctgcgagca gggaaacgct
     1261 cccctcacag acgcgttgaa ttgtccccac gccgcgcccc tgtagagaaa tataaaaggt
     1321 taggatttgc cactgaggtt cttctttcat atacttcctt ttaaaatctt gctaggatac
     1381 agttctcaca tcacatccga acataaacaa ccatgggtaa ggaaaagact cacgtttcga
     1441 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
     1501 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
     1561 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
     1621 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
     1681 atgatgcatg gttactcacc actgcgatcc ccggcaaaac agcattccag gtattagaag
     1741 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
     1801 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
     1861 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
     1921 gctggcctgt tgaacaagtc tggaaagaaa tgcataagct tttgccattc tcaccggatt
     1981 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
     2041 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     2101 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     2161 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     2221 aatcagtact gacaataaaa agattcttgt tttcaagaac ttgtcatttg tatagttttt
     2281 ttatattgta gttgttctat tttaatcaaa tgttagcgtg atttatattt tttttcgcct
     2341 cgacatcatc tgcccagatg cgaagttaag tgcgcagaaa gtaatatcat gcgtcaatcg
     2401 tatgtgaatg ctggtcgcta tactgctgtc gattcgatac taacgccgcc atccagtgtc
     2461 gaaaacgagc tcgaattcct gggggaacaa cttcacagaa tgttttgtca tattgtcgaa
     2521 gtggtcacaa aacaagagaa gttccgccaa ttataaaaag ggaacccgta tatttcagct
     2581 tcacggatga tttccagggt gagagtactg tatatgggct tacgatagaa ggccataaaa
     2641 atttcttgct tggcaacaaa atagaagtga aatcatgtcg aggctgctgt gtgggagaac
     2701 agcttaaaat atcacaaaaa aagaatctaa aacactgtgt tgcttgtccc agaaagggaa
     2761 tcaagtattt ttataaagat tggagtggta aaaatcgagt atgtgctaga tgctatggaa
     2821 gatacaaatt cagcggtcat cactgtataa attgcaagta tgtaccagaa gcacgtgaag
     2881 tgaaaaaggc aaaagacaaa ggcgaaaaat tgggcattac gcccgaaggt ttgccagtta
     2941 aaggaccaga gtgtataaaa tgtggcggaa tcttacagtg gcctatgcgg ccgctctaga
     3001 actagtggat cgatccccaa ttcgccctat agtgagtcgt attacaattc actggccgtc
     3061 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     3121 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     3181 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
     3241 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     3301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     3361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     3421 aagctctaaa tcggggtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3481 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3541 cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3601 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
     3661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     3721 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     3781 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     3841 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     3901 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     3961 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     4021 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     4081 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     4141 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     4201 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     4261 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     4321 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     4381 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     4441 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     4501 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     4561 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     4621 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     4681 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     4741 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     4801 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     4861 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     4921 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     4981 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     5041 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     5101 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     5161 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     5221 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     5281 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     5341 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     5401 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     5461 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     5521 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     5581 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     5641 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     5701 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     5761 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     5821 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     5881 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     5941 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     6001 ttccgcggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     6061 ct
//
