Basic Vector Information
HO-poly-HO vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
HO-poly-HO vector Sequence
LOCUS 40924_1324 4634 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector HO-poly-HO, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4634) AUTHORS Voth WP, Richards JD, Shaw JM, Stillman DJ. TITLE Yeast vectors for integration at the HO locus JOURNAL Nucleic Acids Res. 29 (12), E59 (2001) PUBMED 11410682 REFERENCE 2 (bases 1 to 4634) AUTHORS Voth WP, Richards J, Shaw J, Stillman DJ. TITLE Direct Submission JOURNAL Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 4634) TITLE Direct Submission REFERENCE 4 (bases 1 to 4634) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "2001"; volume: "29"; issue: "12"; pages: "E59" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4634 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 1567..1583 /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(1598..1616) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1623..1639) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2511..2615 /label=AmpR promoter CDS 2616..3473 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3647..4235 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4523..4544 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." protein_bind 4599..4615 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)."
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