LOCUS 40924_1324 4634 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector HO-poly-HO, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4634) AUTHORS Voth WP, Richards JD, Shaw JM, Stillman DJ. TITLE Yeast vectors for integration at the HO locus JOURNAL Nucleic Acids Res. 29 (12), E59 (2001) PUBMED 11410682 REFERENCE 2 (bases 1 to 4634) AUTHORS Voth WP, Richards J, Shaw J, Stillman DJ. TITLE Direct Submission JOURNAL Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA REFERENCE 3 (bases 1 to 4634) TITLE Direct Submission REFERENCE 4 (bases 1 to 4634) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic Acids Res."; date: "2001"; volume: "29"; issue: "12"; pages: "E59" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (29-NOV-2000) Pathology, University of Utah, 50 N. Medical Drive, 5C124 SOM, Salt Lake City, UT 84132, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..4634 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 1567..1583 /label=SK primer /note="common sequencing primer, one of multiple similar variants" promoter complement(1598..1616) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1623..1639) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" promoter 2511..2615 /label=AmpR promoter CDS 2616..3473 /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" rep_origin 3647..4235 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" protein_bind 4523..4544 /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." protein_bind 4599..4615 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." ORIGIN 1 atgaccatga ttacgccact agtccgaggc ctcgagatcc gatatcgccg tggcggccgc 61 cagctgaagc ttaattatcc tgggcacgag tgaaacaaag ctaaaacctt tatttagcat 121 ggccattgaa tgtaacaatt atatatatcg caagcacaaa aaatcaagga gagagaacta 181 ccactttgtt catgtgtaca atgttcatta tctccataag caaaaaaaaa aaatagaaaa 241 catatgctat aaggttgata ttctcacgag taagcggcac ttgctactta ttgacattgc 301 agatttttgg ctacagaaat agtatattag agattataat tgctaatcaa atcaaaatat 361 aaaattagta aaccaaacca tttataccct tccttagtag ttatggattg ttttttaatg 421 atatttctgc aaaccaaaga aagattgtta tccagataga atttagtttt gatattcatt 481 tttttgttga agattgaacg ccatatctgg gcctcataat tcaaaagacg gtgccattat 541 cggtagcgtt tcgcattgta ctggatttca gaaatttcac agttgatgaa tcgaaaagaa 601 tggtctcatt gcaacacgta aggttaagat gtcccttttt accattatag gcaataaatg 661 aatcataaaa cgaccgtata ctggtgaaat agtagggaga acgagtacct gtagtaaaaa 721 gtataaatca tagttaatcg ggcaatgtcc ctcgatcaag gagtattgtg tcatgttcga 781 gacaaacgcc aacatttttg tttcttttgg acaaatgttg tttgcattta tgatccgtta 841 tattttgatc taatgtagag ttgcacgtag ttcttactgg caaagaaatc gatgcatacc 901 aaaaaagaat aaaggtgata tttgatcttt accgtttagt tccaacgtaa aattgtgcct 961 ttggacttaa aatggcgtcg tacgctgcag gtcgacggat ccccggggtt aattaaggcg 1021 cgccagatct gtttaaacga gctcgaattc ctgggggaac aacttcacag aatgttttgt 1081 catattgtcg aagtggtcac aaaacaagag aagttccgcc aattataaaa agggaacccg 1141 tatatttcag cttcacggat gatttccagg gtgagagtac tgtatatggg cttacgatag 1201 aaggccataa aaatttcttg cttggcaaca aaatagaagt gaaatcatgt cgaggctgct 1261 gtgtgggaga acagcttaaa atatcacaaa aaaagaatct aaaacactgt gttgcttgtc 1321 ccagaaaggg aatcaagtat ttttataaag attggagtgg taaaaatcga gtatgtgcta 1381 gatgctatgg aagatacaaa ttcagcggtc atcactgtat aaattgcaag tatgtaccag 1441 aagcacgtga agtgaaaaag gcaaaagaca aaggcgaaaa attgggcatt acgcccgaag 1501 gtttgccagt taaaggacca gagtgtataa aatgtggcgg aatcttacag tggcctatgc 1561 ggccgctcta gaactagtgg atcgatcccc aattcgccct atagtgagtc gtattacaat 1621 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 1681 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 1741 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 1801 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 1861 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 1921 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 1981 gctttccccg tcaagctcta aatcggggtg ggccatcgcc ctgatagacg gtttttcgcc 2041 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac 2101 tcaaccctat ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt 2161 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt 2221 ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc 2281 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg 2341 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat 2401 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca 2461 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc 2521 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 2581 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 2641 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 2701 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 2761 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 2821 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 2881 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 2941 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 3001 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 3061 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 3121 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 3181 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 3241 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 3301 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc 3361 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 3421 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 3481 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 3541 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 3601 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 3661 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 3721 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 3781 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 3841 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 3901 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 3961 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 4021 gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 4081 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 4141 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 4201 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 4261 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt 4321 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga 4381 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc 4441 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 4501 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 4561 tacactttat gcttccgcgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca 4621 cacaggaaac agct //