Basic Vector Information
GST6-LUC-NOS-At2g47730 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GST6-LUC-NOS-At2g47730 vector Sequence
LOCUS 40924_1249 5225 bp DNA circular SYN 17-DEC-2018 DEFINITION Control vector GST6-LUC-NOS-At2g47730, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5225) AUTHORS Kovtun Y, Chiu WL, Tena G, Sheen J. TITLE Functional analysis of oxidative stress-activated mitogen-activated protein kinase cascade in plants JOURNAL Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000) PUBMED 10717008 REFERENCE 2 (bases 1 to 5225) AUTHORS Sheen J. TITLE Direct Submission JOURNAL Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA REFERENCE 3 (bases 1 to 5225) TITLE Direct Submission REFERENCE 4 (bases 1 to 5225) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl. Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: "2940-2945" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, USA" COMMENT SGRef: number: 3; type: "Journal Article" FEATURES Location/Qualifiers source 1..5225 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind complement(15..31) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(39..55) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(63..93) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(108..129) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(417..1005) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(1179..2036) /label=AmpR /note="beta-lactamase" promoter complement(2037..2141) /label=AmpR promoter primer_bind 2615..2631 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 2667..3161 /label=GST6 enhancer/promoter /note="GST6 enhancer/promoter" /regulatory_class="enhancer" CDS 3162..4811 /label=luciferase /note="firefly luciferase" terminator 4969..5220 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal"
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