LOCUS       40924_1249        5225 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Control vector GST6-LUC-NOS-At2g47730, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5225)
  AUTHORS   Kovtun Y, Chiu WL, Tena G, Sheen J.
  TITLE     Functional analysis of oxidative stress-activated mitogen-activated 
            protein kinase cascade in plants
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 97 (6), 2940-2945 (2000)
  PUBMED    10717008
REFERENCE   2  (bases 1 to 5225)
  AUTHORS   Sheen J.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-OCT-2006) Molecular Biology, Massachusetts General 
            Hospital, 185 Cambridge St. Simches Research Building-CPZN7600, 
            Boston, MA 02114, USA
REFERENCE   3  (bases 1 to 5225)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5225)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2000"; volume: "97"; issue: "6"; pages: 
            "2940-2945"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-OCT-2006) Molecular Biology, Massachusetts General Hospital, 185
            Cambridge St. Simches Research Building-CPZN7600, Boston, MA 02114, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5225
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(15..31)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(39..55)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(63..93)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(108..129)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(417..1005)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1179..2036)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2037..2141)
                     /label=AmpR promoter
     primer_bind     2615..2631
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      2667..3161
                     /label=GST6 enhancer/promoter
                     /note="GST6 enhancer/promoter"
                     /regulatory_class="enhancer"
     CDS             3162..4811
                     /label=luciferase
                     /note="firefly luciferase"
     terminator      4969..5220
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
       61 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      121 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
      181 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
      241 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      301 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      361 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      421 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      481 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      541 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      601 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      661 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      721 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      781 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      841 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      901 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      961 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     1021 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     1081 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     1141 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1201 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1261 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1321 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1381 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1441 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1501 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1561 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1621 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1681 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1741 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1801 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1861 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1921 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1981 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     2041 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     2101 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     2161 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     2221 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     2281 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     2341 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     2401 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     2461 taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     2521 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     2581 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgccaagctt
     2641 gcatgcctgc aggctctaga ggatcctcta gaatcttatg tcattgatga cgacctcctc
     2701 tacacttttg gatttgtatg tttacaaagg aaggaaggat taccttagaa cagtttatgt
     2761 gatgtttgtg tctttctatt tttattcaat gaaatttctt tacatgtaca cagattagct
     2821 atattgacat gaatctaact gatttttttg tttgcaaaat aacaaattag tgaggggaat
     2881 tttgtttcag tcaataaatc cgtgatttct atatgttaga tggataacaa tgcaataaat
     2941 aaaggggacc caataaaaaa aaaggaaaga gagacgtgcc tgtgggtagt gggtggtggt
     3001 gagaagattc tggaaaaatg ggagcaattc aagctcgtct tcccttgttc ctttctcccc
     3061 cttctataaa acaccatacc ttccttcatt cttcttcatc caattccaat ttcaaaatca
     3121 gatccaacaa atcttcttct tcttcttctt cttcgatcac catggaagac gccaaaaaca
     3181 taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc
     3241 ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata
     3301 tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta
     3361 tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc
     3421 aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg
     3481 acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt
     3541 ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc
     3601 agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt
     3661 tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg
     3721 atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg
     3781 gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt
     3841 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt
     3901 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata
     3961 gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc
     4021 tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat
     4081 ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg
     4141 ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact gagactacat
     4201 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc
     4261 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga
     4321 gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag
     4381 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg
     4441 acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat
     4501 atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac atcttcgacg
     4561 cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt
     4621 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa
     4681 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta
     4741 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa
     4801 agtccaaatt gtaaaatgta actgtattca gcgatgacga aattcttagc tattgtaata
     4861 ttatatgcaa attgatgaat ggtaattttg taattgtggg tcactgtact attttaacga
     4921 ataataaaat caggtatagg taactaaaaa ggaattcgag ctcgaattga tcgttcaaac
     4981 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
     5041 taatttctgt tgattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     5101 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     5161 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     5221 tctag
//
