GmScreamM8L-pFLEV vector (V009935)

Basic Vector Information

      • Vector Name:
      • GmScreamM8L-pFLEV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6342 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Zhang N, McHale LK, Finer JJ.

GmScreamM8L-pFLEV vector Vector Map

GmScreamM8L-pFLEV6342 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300M13 fwdGmScreamM8L promotersGFP65TNOS terminatorM13 revlac operatorlac promoterCAP binding sitederived from ampicillin geneAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GmScreamM8L-pFLEV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6342 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM8L-pFLEV, complete sequence.
ACCESSION   KX252737
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 6342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Cloning vector GmScreamM8L-pFLEV"
                     /mol_type="other DNA"
                     /label=derived from Glycine max
                     /note="derived from Glycine max"
                     /db_xref="taxon:1890329"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..3059
                     /regulatory_class="promoter"
                     /note="GmScreamM8L promoter"
     CDS             3062..3781
                     /codon_start=1
                     /product="sGFP65T"
                     /label=sGFP65T
                     /protein_id="AOC59760.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     regulatory      3814..4089
                     /regulatory_class="terminator"
                     /note="NOS terminator"
     terminator      3814..4066
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4121..4137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4145..4161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4169..4199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4214..4235
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(4523..5111)
                     /direction=LEFT
     misc_feature    complement(5282..6142)
                     /label=derived from ampicillin gene
                     /note="derived from ampicillin gene"
     CDS             complement(5282..6142)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6143..6247)
                     /gene="bla"
                     /label=AmpR promoter

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