LOCUS 40924_1184 6342 bp DNA circular SYN 17-DEC-2018 DEFINITION Cloning vector GmScreamM8L-pFLEV, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6342) AUTHORS Zhang N, McHale LK, Finer JJ. TITLE Isolation and characterization of 'GmScream' promoters that regulate highly expressing soybean (Glycine max Merr.) genes JOURNAL Plant Sci. 241, 189-198 (2015) PUBMED 26706070 REFERENCE 2 (bases 1 to 6342) AUTHORS Zhang N. TITLE Direct Submission JOURNAL Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA REFERENCE 3 (bases 1 to 6342) TITLE Direct Submission REFERENCE 4 (bases 1 to 6342) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 241, 189-198 (2015)" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 1680 Madison Ave, Wooster, OH 44691, USA" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..6342 /mol_type="other DNA" /organism="synthetic DNA construct" primer_bind 379..395 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" regulatory 405..3059 /label=GmScreamM8L promoter /note="GmScreamM8L promoter" /regulatory_class="promoter" CDS 3062..3778 /label=EGFP /note="enhanced GFP" terminator 3814..4066 /label=NOS terminator /note="nopaline synthase terminator and poly(A) signal" primer_bind complement(4121..4137) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" protein_bind complement(4145..4161) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4169..4199) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(4214..4235) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." rep_origin complement(4523..5111) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5285..6142) /label=AmpR /note="beta-lactamase" promoter complement(6143..6247) /label=AmpR promoter ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcataa tgcccacaca 421 atccatcggc aaaaaaacca acaatctatt aaacgcttct tctcaatctc aattataaca 481 cgcaagtcac attcataaag atatatttac tacacattta tcaacaataa aaccaaaaat 541 aaataaataa aacaataatc aacttatcaa aaaataaaat tataaataaa acataaaaac 601 acctacagcc aaaaaatcaa acggtatgta taaaaaaaag aaaagaaaaa caaaggaaaa 661 aacataaaaa caaactttgc aactccatca aaccaccacc gtgatttgat catcagcacg 721 gcttgaaatt tcaacaatta aatacaaaca acaaagaatg acaatcaatt aatcaggcga 781 gaagaaatca gttgcaatgc gaatttcaat ttcacaattt atagaggata gaaaagtagt 841 aaaaaatgat tttttaaaaa agaaatcaaa atcaaaataa ataacaatca aattatatat 901 gtaaagcaat tacagtttat caaactttat ttatggaaat aatttattat cacatttatt 961 ttggtttata aattttaaat taaaatatca cctaaataaa aataattttt aacatgactt 1021 attgtcctaa ataaattatt tccgtaaatt aaataaaatg aagttttttt ctttcaaaga 1081 atctaaatgg tcataatgag aattctctaa aaaaatacat aatgagaata attatggaat 1141 ttatttatta ataaaaatta atagcatttt gatagacaat taataaaatt ttaaaaataa 1201 ccatatagaa ataataattt ttttactatc ggttccaatt aaaataatga taaaaaataa 1261 aatagattat taattgatat tgatatgaaa tttaaataaa gaatataatc atatatttta 1321 ttgatatatg atatgatata gattaattga tattgatttt gatatggaat ttaaaaataa 1381 tataataatt gtttttattt attaatacgt gtaatcaaat aattctcact ttttgaatca 1441 atcagtgtac ttaaagataa tatcagttga atatttttta tccttttacg tgtgctgtga 1501 gacattatca tcaattgtgt tgtatatgat atatagatat agatatataa atatatagat 1561 tgagtgatat aatatattta aaatataaat tatatatatg ttttaatata tttttgcata 1621 tatatatata tttgtaaaaa ctagaagtat tttttcatga gataattatt atcgagttga 1681 ataagtctat tatttgtgag agccaaccat atttatatat gtgattaaat tttatctttg 1741 tgaaattaaa aataataaaa aataccttaa aatcataata atagaaaaac ttatatttat 1801 aatttaccat tatacttaaa aaaaattaaa taaatattat aaatataaat actatcgagt 1861 aatggccgcg ctagggtttt tgagaaaaaa tcttcccacg cactcaactg cactgtacgg 1921 cgtcgttttc acagccgcat aatagaagcc gcgttcccca acccttcctc acaacattct 1981 cggaccctcc agcaccgtca cccaaacaaa tatccacgcg gtagtaggcg cgtgaaacaa 2041 actctaatcc gaactacgag acgtgagaag cacgcgcttt agcgagcgtt tcaattgtcg 2101 ctacgaaagc agagaaggat acaaacggaa ctagggtaaa ttagtaaggg taatttcgta 2161 aacagaagaa aagagttgta gctataaata aaccctctaa ccctcgtcgc attacttctc 2221 ttcacacctt tgttcactct tcttctcttg cggctagggt tttagcgcag cttcttctag 2281 gttcgttatc taccaccgtt ctatggattt tattccttct attcgtgttt attctattgg 2341 tttatgttgc ttgcaatatg ttttttctga atctgtcgtc gttgtcttca attttatcca 2401 tgtttcagag atcaattttg tttgtgtagt atgtgcttat tcttcttctt ttcgttcgag 2461 ttgttaataa cggtgctatg gtgttttcaa aagtgttttt tttattactt ttgatttaaa 2521 gtttttttgg taaggctttt atttgcttgt tatattcaaa tctttggatc cagatcttat 2581 ataagttttt ggttcaagaa agtttttggt tactgatgaa tagatctatt aactgttact 2641 ttaatcgatt caagctaaag ttttttggtt actgatgaat agatctatta tctgttactt 2701 ttaatcggtt caagctcaag ttttttggtt actgatgaat agatctatat acgtcacagt 2761 gtgctaaaca tgcccttgtt ttatctcgat cttatgtatg ggagtgccat aaattttgtt 2821 atgtctattt ttttatctgt tggaatcata ctgagtttga tgcgttacga ttgagcatac 2881 ctatttttgg gcttgttgta tggtgggtat ttagatctta atctttttat gcttatgaaa 2941 ggttttgtaa tgacaaaggt cttaatgttg ttaaactttt atttttactt tatatggtgt 3001 gttgatgtgt tatggttttg acaacttttt ttttttctgg atttttgcag atttaaggac 3061 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga 3121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta 3181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac 3241 cctcgtgacc accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa 3301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt 3361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct 3421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca 3481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa 3541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc 3601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca 3661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt 3721 cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta 3781 aagcggccgc ccggctgcac tgaggcctca gcagatcgtt caaacatttg gcaataaagt 3841 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat 3901 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt 3961 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca 4021 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatccgat gataagctgt 4081 caaacatgag aattagatct caattgaatt cgtaatcatg gtcatagctg tttcctgtgt 4141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag 4201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt 4261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag 4321 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg 4381 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat 4441 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta 4501 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa 4561 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc 4621 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt 4681 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca 4741 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg 4801 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat 4861 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta 4921 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct 4981 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac 5041 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa 5101 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa 5161 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt 5221 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca 5281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca 5341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc 5401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa 5461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc 5521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca 5581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat 5641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag 5701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac 5761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt 5821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt 5881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc 5941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat 6001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca 6061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga 6121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg 6181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 6241 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga 6301 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc //