LOCUS       40924_1184        6342 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM8L-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 6342)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6342)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..3059
                     /label=GmScreamM8L promoter
                     /note="GmScreamM8L promoter"
                     /regulatory_class="promoter"
     CDS             3062..3778
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      3814..4066
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4121..4137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(4145..4161)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4169..4199)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4214..4235)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4523..5111)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5285..6142)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6143..6247)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttgcataa tgcccacaca
      421 atccatcggc aaaaaaacca acaatctatt aaacgcttct tctcaatctc aattataaca
      481 cgcaagtcac attcataaag atatatttac tacacattta tcaacaataa aaccaaaaat
      541 aaataaataa aacaataatc aacttatcaa aaaataaaat tataaataaa acataaaaac
      601 acctacagcc aaaaaatcaa acggtatgta taaaaaaaag aaaagaaaaa caaaggaaaa
      661 aacataaaaa caaactttgc aactccatca aaccaccacc gtgatttgat catcagcacg
      721 gcttgaaatt tcaacaatta aatacaaaca acaaagaatg acaatcaatt aatcaggcga
      781 gaagaaatca gttgcaatgc gaatttcaat ttcacaattt atagaggata gaaaagtagt
      841 aaaaaatgat tttttaaaaa agaaatcaaa atcaaaataa ataacaatca aattatatat
      901 gtaaagcaat tacagtttat caaactttat ttatggaaat aatttattat cacatttatt
      961 ttggtttata aattttaaat taaaatatca cctaaataaa aataattttt aacatgactt
     1021 attgtcctaa ataaattatt tccgtaaatt aaataaaatg aagttttttt ctttcaaaga
     1081 atctaaatgg tcataatgag aattctctaa aaaaatacat aatgagaata attatggaat
     1141 ttatttatta ataaaaatta atagcatttt gatagacaat taataaaatt ttaaaaataa
     1201 ccatatagaa ataataattt ttttactatc ggttccaatt aaaataatga taaaaaataa
     1261 aatagattat taattgatat tgatatgaaa tttaaataaa gaatataatc atatatttta
     1321 ttgatatatg atatgatata gattaattga tattgatttt gatatggaat ttaaaaataa
     1381 tataataatt gtttttattt attaatacgt gtaatcaaat aattctcact ttttgaatca
     1441 atcagtgtac ttaaagataa tatcagttga atatttttta tccttttacg tgtgctgtga
     1501 gacattatca tcaattgtgt tgtatatgat atatagatat agatatataa atatatagat
     1561 tgagtgatat aatatattta aaatataaat tatatatatg ttttaatata tttttgcata
     1621 tatatatata tttgtaaaaa ctagaagtat tttttcatga gataattatt atcgagttga
     1681 ataagtctat tatttgtgag agccaaccat atttatatat gtgattaaat tttatctttg
     1741 tgaaattaaa aataataaaa aataccttaa aatcataata atagaaaaac ttatatttat
     1801 aatttaccat tatacttaaa aaaaattaaa taaatattat aaatataaat actatcgagt
     1861 aatggccgcg ctagggtttt tgagaaaaaa tcttcccacg cactcaactg cactgtacgg
     1921 cgtcgttttc acagccgcat aatagaagcc gcgttcccca acccttcctc acaacattct
     1981 cggaccctcc agcaccgtca cccaaacaaa tatccacgcg gtagtaggcg cgtgaaacaa
     2041 actctaatcc gaactacgag acgtgagaag cacgcgcttt agcgagcgtt tcaattgtcg
     2101 ctacgaaagc agagaaggat acaaacggaa ctagggtaaa ttagtaaggg taatttcgta
     2161 aacagaagaa aagagttgta gctataaata aaccctctaa ccctcgtcgc attacttctc
     2221 ttcacacctt tgttcactct tcttctcttg cggctagggt tttagcgcag cttcttctag
     2281 gttcgttatc taccaccgtt ctatggattt tattccttct attcgtgttt attctattgg
     2341 tttatgttgc ttgcaatatg ttttttctga atctgtcgtc gttgtcttca attttatcca
     2401 tgtttcagag atcaattttg tttgtgtagt atgtgcttat tcttcttctt ttcgttcgag
     2461 ttgttaataa cggtgctatg gtgttttcaa aagtgttttt tttattactt ttgatttaaa
     2521 gtttttttgg taaggctttt atttgcttgt tatattcaaa tctttggatc cagatcttat
     2581 ataagttttt ggttcaagaa agtttttggt tactgatgaa tagatctatt aactgttact
     2641 ttaatcgatt caagctaaag ttttttggtt actgatgaat agatctatta tctgttactt
     2701 ttaatcggtt caagctcaag ttttttggtt actgatgaat agatctatat acgtcacagt
     2761 gtgctaaaca tgcccttgtt ttatctcgat cttatgtatg ggagtgccat aaattttgtt
     2821 atgtctattt ttttatctgt tggaatcata ctgagtttga tgcgttacga ttgagcatac
     2881 ctatttttgg gcttgttgta tggtgggtat ttagatctta atctttttat gcttatgaaa
     2941 ggttttgtaa tgacaaaggt cttaatgttg ttaaactttt atttttactt tatatggtgt
     3001 gttgatgtgt tatggttttg acaacttttt ttttttctgg atttttgcag atttaaggac
     3061 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     3121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     3181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     3241 cctcgtgacc accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     3301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     3361 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     3421 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     3481 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     3541 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     3601 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     3661 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
     3721 cctgctggag ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta
     3781 aagcggccgc ccggctgcac tgaggcctca gcagatcgtt caaacatttg gcaataaagt
     3841 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3901 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3961 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     4021 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatccgat gataagctgt
     4081 caaacatgag aattagatct caattgaatt cgtaatcatg gtcatagctg tttcctgtgt
     4141 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     4201 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     4261 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     4321 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     4381 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     4441 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     4501 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     4561 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     4621 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     4681 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     4741 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     4801 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     4861 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     4921 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     4981 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     5041 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     5101 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     5161 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     5221 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     5281 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     5341 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     5401 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     5461 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     5521 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     5581 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     5641 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     5701 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     5761 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     5821 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     5881 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     5941 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     6001 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     6061 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     6121 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     6181 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     6241 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     6301 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
