GmScreamM5-pFLEV vector (V009940)

Basic Vector Information

      • Vector Name:
      • GmScreamM5-pFLEV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4939 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Zhang N, McHale LK, Finer JJ.

GmScreamM5-pFLEV vector Vector Map

GmScreamM5-pFLEV4939 bp6001200180024003000360042004800M13 fwdGmScreamM5 promotersGFP65TNOS terminatorM13 revlac operatorlac promoterCAP binding sitederived from ampicillin geneAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GmScreamM5-pFLEV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4939 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM5-pFLEV, complete sequence.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4939)
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Cloning vector GmScreamM5-pFLEV"
                     /mol_type="other DNA"
                     /label=derived from Glycine max
                     /note="derived from Glycine max"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
     regulatory      411..1656
                     /note="GmScreamM5 promoter"
     CDS             1659..2378
     regulatory      2411..2686
                     /note="NOS terminator"
     terminator      2411..2663
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2718..2734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
     protein_bind    2742..2758
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2766..2796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2811..2832
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(3120..3708)
     misc_feature    complement(3879..4739)
                     /label=derived from ampicillin gene
                     /note="derived from ampicillin gene"
     CDS             complement(3879..4739)
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     promoter        complement(4740..4844)
                     /label=AmpR promoter

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