LOCUS       40924_1159        4939 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScreamM5-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 4939)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4939)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      411..1656
                     /label=GmScreamM5 promoter
                     /note="GmScreamM5 promoter"
                     /regulatory_class="promoter"
     CDS             1659..2375
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2411..2663
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2718..2734)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2742..2758)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2766..2796)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2811..2832)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3120..3708)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3882..4739)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4740..4844)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttctgcag caccatcacc
      421 actaacagtt cactttgcat tggttaaact ggttttatat tttcttaatt ttatataata
      481 ttttaatagt aattaaatga taatatagat aaaaaataat aaaaacttta gaattttaaa
      541 acatcaataa tttaaaaaga aaacctaaaa cattaaatta ctgataataa ctattagttt
      601 cctcatcgtg caaaaaccaa tttcgatcct ctccaactca attttcttga atcatcacgc
      661 tgtcagaatt taatccttaa gctttacaac aaaaatattt aaatcgctaa atttattatt
      721 atacaataat tagagagaaa agaatataac gagtaaatta ttattttagc ttctaaaagt
      781 gtaatgccct atcacattag tttcttaaac tgttttttaa aaacaaaatc atgaaatttt
      841 caattattca tctaagtctt taaatttaac aaacagcaac agtaataatg cgaaaaccca
      901 tccaatcaaa tacagaaagc tatatccatg tatttcctta aatcaaccca accatcttta
      961 attcaaaaaa ataggtcttg aacagaattg ttaaaaaagt aagttcttta agtcgattta
     1021 cgtcgacttg gtcggcatcc aacaactcct caaacgaaag gccgccataa ttctggggag
     1081 tgctcgatcg accaaaactg gggaatattt tcaataagat ctaaaatttt ctttgtaggc
     1141 ttaggccaaa gaacttatct ttgaagcctt tgtatgacca agtatacaaa ttcaatagtc
     1201 tctgtccgtg gacactattg accgacaccc atgctgtttt tttttttgtg tgtgtttgta
     1261 aattcaaaaa aataaatttt tttattagtc aggcacaatt gctattccta ggcaacacat
     1321 agaaccttga aataccaaac aaacacccaa ttattattgt gtgtctggat gaagcaatta
     1381 aagtagataa aatattttat ttgcgatatt aaaatgcctt tttataaaat aatttgtttg
     1441 gataaaatat tttaaaagta attaaaatgt cattattttt taaagcattt taatagacta
     1501 aattgggaga attttaaatt cttacaaaaa atagaaaaat taaaattctc catccacaaa
     1561 cacgctcaga gacctctctt cccctctgag ccacttacgc aacgctctct tcccttctca
     1621 gccactctct cacgctcttt cccctctcag ccaccaccat ggtgagcaag ggcgaggagc
     1681 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
     1741 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
     1801 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcacctacg
     1861 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
     1921 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
     1981 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
     2041 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     2101 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     2161 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     2221 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     2281 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     2341 ccgggatcac tcacggcatg gacgagctgt acaagtaaag cggccgcccg gctgcactga
     2401 ggcctcagca gatcgttcaa acatttggca ataaagtttc ttaagattga atcctgttgc
     2461 cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg taataattaa
     2521 catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc cgcaattata
     2581 catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat tatcgcgcgc
     2641 ggtgtcatct atgttactag atccgatgat aagctgtcaa acatgagaat tagatctcaa
     2701 ttgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     2761 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     2821 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2881 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2941 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3001 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3061 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3121 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     3181 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     3241 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     3301 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     3361 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     3421 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     3481 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     3541 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     3601 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     3661 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     3721 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     3781 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     3841 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     3901 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     3961 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4021 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4081 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4141 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     4201 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     4261 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     4321 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     4381 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     4441 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     4501 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     4561 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     4621 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     4681 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     4741 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     4801 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     4861 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     4921 atcacgaggc cctttcgtc
//
