GmScream10-pFLEV vector (V009954)

Basic Vector Information

      • Vector Name:
      • GmScream10-pFLEV
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 5101 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Zhang N, McHale LK, Finer JJ.

GmScream10-pFLEV vector Vector Map

GmScream10-pFLEV5101 bp6001200180024003000360042004800M13 fwdGmScream10 promoterEGFPNOS terminatorM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GmScream10-pFLEV vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_1089        5101 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream10-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1818
                     /label=GmScream10 promoter
                     /note="GmScream10 promoter"
                     /regulatory_class="promoter"
     CDS             1821..2537
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2573..2825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2880..2896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2904..2920)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2928..2958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2973..2994)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3282..3870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4044..4901)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4902..5006)
                     /label=AmpR promoter

This page is informational only.