LOCUS       40924_1089        5101 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GmScream10-pFLEV, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5101)
  AUTHORS   Zhang N, McHale LK, Finer JJ.
  TITLE     Isolation and characterization of 'GmScream' promoters that regulate
            highly expressing soybean (Glycine max Merr.) genes
  JOURNAL   Plant Sci. 241, 189-198 (2015)
  PUBMED    26706070
REFERENCE   2  (bases 1 to 5101)
  AUTHORS   Zhang N.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-MAY-2016) Horticulture and Crop Science, Ohio State 
            University, 1680 Madison Ave, Wooster, OH 44691, USA
REFERENCE   3  (bases 1 to 5101)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5101)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Sci. 
            241, 189-198 (2015)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-MAY-2016) Horticulture and Crop Science, Ohio State University, 
            1680 Madison Ave, Wooster, OH 44691, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..5101
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      405..1818
                     /label=GmScream10 promoter
                     /note="GmScream10 promoter"
                     /regulatory_class="promoter"
     CDS             1821..2537
                     /label=EGFP
                     /note="enhanced GFP"
     terminator      2573..2825
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2880..2896)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2904..2920)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2928..2958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2973..2994)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3282..3870)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4044..4901)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4902..5006)
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgccaa gcttatttcc gtacacttcc
      421 gtgcacctcc cttcctctct tctctatttc gtgccactaa acacgctaaa tcccaaacat
      481 ataaaaacct aacacatgta tatgcattta tttcttctac acatgtataa ggggtctctt
      541 tgaggtaaca aggcaaagca taatgataca tttattacat gtaatcaaat aaaatgtttg
      601 gttttggaca taattttaaa atgttctagt tcaattttta ttgtagtttt aaagcaatag
      661 acctcttctt attttcgtga taaatgaatc aattgcaaaa ttagttatac attaacatct
      721 cattgatttt ataagaacta ctacattttg aaagttagtt gtagcaatat tttcagttta
      781 caattgcctt gagagttagc tcttggacta ataagtaaga atgtttaaaa ctaaatcaaa
      841 tcgtttgtaa atcaccaaat taatataaaa aataaaaaat tgcttaaacc aaaaataaca
      901 aaaaaaaaac atatattttt tgtaatttag tttttggatc ttattataaa aaataaatca
      961 agccgaacaa aatgtatatt ataattatat taatttctat tgtgataatt aataataagt
     1021 attagataat ttaaattttc acttacttta taattaatat gtacatttta tttttatatg
     1081 ttaaaaaaga tagtaataat attgttaaaa agtagtgtta aatattaaat gatgtacaaa
     1141 ttattattat attaaaaaag ctggtaacat tattttatat taaatagaag tcaagataat
     1201 aatttttaac taaaatatgt taaaaaaaaa aataaaaaaa ttaattttta aaattatata
     1261 aaaagtatat aataagtatg taagtttact agataaattt aaattatgat aataggttaa
     1321 aaaataaaac aaatatgaaa ttgaactaaa ttaaaaaaaa tgatttagtt taattcgaat
     1381 ttgatattat attaaaatta aatcacatct gtattttata tttaatttga ataattttta
     1441 tctaaaaatt aaatcaaatc acgttaagaa caccctacta ataaagtggc aatgcaatta
     1501 agactttggg tttcggttga gtcacaaaga aaaacagtat gactatgtaa atatgggtcc
     1561 agttttattt gggcttagac cttagataaa gtgtttaatt gtgacaactc caataaatag
     1621 aatgcaacgt agttcaaatg tgtgtctggt tttttatttt ataaataaat atataccaat
     1681 accacaccaa gcattggtaa gggtccaaga ctccaagtgt tcaacatcga agaatgcatg
     1741 catgcaagag aattttgtta taaatttaat atccgttgga tttattaacg ccggtttccc
     1801 gaacgatttg atggtttgcc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     1861 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     1921 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     1981 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc
     2041 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     2101 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     2161 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     2221 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     2281 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     2341 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     2401 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     2461 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actcacggca
     2521 tggacgagct gtacaagtaa agcggccgcc cggctgcact gaggcctcag cagatcgttc
     2581 aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat
     2641 catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt
     2701 atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga
     2761 aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact
     2821 agatccgatg ataagctgtc aaacatgaga attagatctc aattgaattc gtaatcatgg
     2881 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     2941 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     3001 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     3061 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     3121 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     3181 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     3241 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     3301 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     3361 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     3421 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     3481 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     3541 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     3601 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     3661 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     3721 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     3781 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     3841 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     3901 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     3961 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     4021 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     4081 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     4141 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     4201 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     4261 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     4321 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     4381 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     4441 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     4501 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     4561 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     4621 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     4681 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     4741 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     4801 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     4861 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     4921 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     4981 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     5041 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     5101 c
//
