GloSponge vector (V009956)

Basic Vector Information

      • Vector Name:
      • GloSponge
      • Length:
      • 6171 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Choudhary A, Fernandez SL-V., Taft RJ.

GloSponge vector Vector Map

GloSponge6171 bp30060090012001500180021002400270030003300360039004200450048005100540057006000SV40 poly(A) signalSV40 promoterEGFPsynthetic polyadenylation signalAmpR promoteramporicer regionPGK promotervertebrate consensus sequence for strong initiation of translation (Kozak, 1987)

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GloSponge vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                6171 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GloSponge, complete sequence.
ACCESSION   KM597055
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Choudhary A, Fernandez SL-V., Taft RJ.
  TITLE     GloSponge: an integrated small RNA sponge and sensor vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Choudhary A, Fernandez SL-V., Taft RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2014) Institute for Molecular Bioscience, 
            University of Queensland, Building 80, Services Road, Brisbane, 
            Queensland 4072, Australia
REFERENCE   3  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6171
                     /organism="Cloning vector GloSponge"
                     /mol_type="other DNA"
                     /db_xref="taxon:1586245"
     polyA_signal    115..236
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        522..819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        620..830
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      689..824
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             829..1548
                     /codon_start=1
                     /gene="EGFP"
                     /product="enhanced green fluorescent protein"
                     /label=EGFP
                     /protein_id="AJA32156.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     gene            829..1548
                     /gene="EGFP"
                     /label=EGFP
     polyA_signal    1549..1597
                     /note="synthetic polyadenylation signal"
     promoter        1753..1857
                     /gene="bla"
                     /label=AmpR promoter
     CDS             1858..2718
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /protein_id="AJA32154.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            1858..2718
                     /gene="amp"
                     /label=amp
     rep_origin      complement(2876..3464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3580..3863
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
     promoter        3920..4419
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     regulatory      4460..4469
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4466..6118
                     /codon_start=1
                     /gene="luc2"
                     /product="firefly luciferase protein"
                     /label=luc2
                     /protein_id="AJA32155.1"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     gene            4466..6118
                     /gene="luc2"
                     /label=luc2

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