LOCUS       40924_1074        6171 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector GloSponge, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6171)
  AUTHORS   Choudhary A, Fernandez SL-V., Taft RJ.
  TITLE     GloSponge: an integrated small RNA sponge and sensor vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6171)
  AUTHORS   Choudhary A, Fernandez SL-V., Taft RJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-SEP-2014) Institute for Molecular Bioscience, 
            University of Queensland, Building 80, Services Road, Brisbane, 
            Queensland 4072, Australia
REFERENCE   3  (bases 1 to 6171)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6171)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (22-SEP-2014) Institute for Molecular Bioscience, University of 
            Queensland, Building 80, Services Road, Brisbane, Queensland 4072, 
            Australia"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6171
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(115..236)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        522..819
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             829..1545
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    1549..1597
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        1753..1857
                     /label=AmpR promoter
     CDS             1858..2715
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      complement(2876..3464)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    3580..3863
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"
     promoter        3920..4419
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4466..6115
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
ORIGIN
        1 catgcaagct gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac
       61 cgctgagcaa taactagcat aaccccttgg ggcggccgct tcgagcagac atgataagat
      121 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
      181 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
      241 acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag gtttttttaa
      301 gcaagtaaaa cctctacaaa tgtggtaaaa tcgaatttta acaaaatatt aacgcttaca
      361 atttcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgcg
      421 gatctgcgca gcaccatggc ctgaaataac ctctgaaaga ggaacttggt taggtacctt
      481 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
      541 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
      601 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
      661 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      721 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      781 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggat ggtgagcaag
      841 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      901 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      961 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
     1021 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
     1081 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
     1141 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
     1201 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
     1261 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
     1321 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
     1381 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
     1441 cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
     1501 gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaaaa taaaatatct
     1561 ttattttcat tacatctgtg tgttggtttt ttgtgtgaat cgatagcgat aaggatcctc
     1621 tttgcgcttg cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag
     1681 caccgtttct gcggactggc tttctacgta atggtttctt agacgtcagg tggcactttt
     1741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1801 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1861 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1921 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1981 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2041 gaacgttttc caatgatgag cactttcaaa gttctgctat gtggcgcggt attatcccgt
     2101 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2161 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2221 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aactatcgga
     2281 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2341 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2401 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2461 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2521 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2581 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2641 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2701 ctgattaagc attggtaatt cgaaatgacc gaccaagcga cgcccaaccg gtatcagctc
     2761 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     2821 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     2881 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     2941 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     3001 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     3061 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     3121 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     3181 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     3241 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     3301 acggctacac tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     3361 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     3421 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatt tcaagaagat cctttgatct
     3481 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     3541 gattatcaaa aaggatcttc acctagatcc ttttatagtc cggaaataca ggaacgcacg
     3601 ctggatggcc cttcgctggg atggtgaaac catgaaaaat ggcagcttca gtggattaag
     3661 tgggggtaat gtggcctgta ccctctggtt gcataggtat tcatacggtt aaaatttatc
     3721 aggcgcgatt gcggcagttt ttcgggtggt ttgttgccat ttttacctgt ctgctgccgt
     3781 gatcgcgctg aacgcgtttt agcggtgcgt acaattaagg gattatggta aatccactta
     3841 ctgtctgccc tcgtagccat cgagataaac cgcagtactc cggccacgat gcgtccggcg
     3901 tagaggatcg agatctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     3961 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     4021 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     4081 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     4141 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4201 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4261 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4321 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4381 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttggca
     4441 atccggtact gttggtaaag ccaccatgga agatgccaaa aacattaaga agggcccagc
     4501 gccattctac ccactcgaag acgggaccgc cggcgagcag ctgcacaaag ccatgaagcg
     4561 ctacgccctg gtgcccggca ccatcgcctt taccgacgca catatcgagg tggacattac
     4621 ctacgccgag tacttcgaga tgagcgttcg gctggcagaa gctatgaagc gctatgggct
     4681 gaatacaaac catcggatcg tggtgtgcag cgagaatagc ttgcagttct tcatgcccgt
     4741 gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct aacgacatct acaacgagcg
     4801 cgagctgctg aacagcatgg gcatcagcca gcccaccgtc gtattcgtga gcaagaaagg
     4861 gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc atacaaaaga tcatcatcat
     4921 ggatagcaag accgactacc agggcttcca aagcatgtac accttcgtga cttcccattt
     4981 gccacccggc ttcaacgagt acgacttcgt gcccgagagc ttcgaccggg acaaaaccat
     5041 cgccctgatc atgaacagta gtggcagtac cggattgccc aagggcgtag ccctaccgca
     5101 ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc atcttcggca accagatcat
     5161 ccccgacacc gctatcctca gcgtggtgcc atttcaccac ggcttcggca tgttcaccac
     5221 gctgggctac ttgatctgcg gctttcgggt cgtgctcatg taccgcttcg aggaggagct
     5281 attcttgcgc agcttgcaag actataagat tcaatctgcc ctgctggtgc ccacactatt
     5341 tagcttcttc gctaagagca ctctcatcga caagtacgac ctaagcaact tgcacgagat
     5401 cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag gccgtggcca aacgcttcca
     5461 cctaccaggc atccgccagg gctacggcct gacagaaaca accagcgcca ttctgatcac
     5521 ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg gtgcccttct tcgaggctaa
     5581 ggtggtggac ttggacaccg gtaagacact gggtgtgaac cagcgcggcg agctgtgcgt
     5641 ccgtggcccc atgatcatga gcggctacgt taacaacccc gaggctacaa acgctctcat
     5701 cgacaaggac ggctggctgc acagcggcga catcgcctac tgggacgagg acgagcactt
     5761 cttcatcgtg gaccggctga agagcctgat caaatacaag ggctaccagg tagccccagc
     5821 cgaactggag agcatcctgc tgcaacaccc caacatcttc gacgccgggg tcgccggcct
     5881 gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc gtgctggaac acggtaaaac
     5941 catgaccgag aaggagatcg tggactatgt ggccagccag gttacaaccg ccaagaagct
     6001 gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga ctgaccggca agttggacgc
     6061 ccgcaagatc cgcgagattc tcattaaggc caagaagggc ggcaagatcg ccgtgtaatt
     6121 ctagttgttt aaacgagctc gctagcctcg agtctagagt cgacctgcag g
//
