Basic Vector Information
GFPuv-reporter vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
GFPuv-reporter vector Sequence
LOCUS 40924_1064 3070 bp DNA circular SYN 17-DEC-2018
DEFINITION Expression vector GFPuv-reporter, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3070)
AUTHORS Han T, Chen Q, Liu H.
TITLE Engineered photoactivatable genetic switches based on the bacterium
phage T7 RNA polymerase
JOURNAL ACS Synth Biol (2016) In press
PUBMED 27794600
REFERENCE 2 (bases 1 to 3070)
AUTHORS Han T, Chen Q, Liu H.
TITLE Direct Submission
JOURNAL Submitted (11-OCT-2016) School of Life Sciences, University of
Science and Technology of China, University of Science and
Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE 3 (bases 1 to 3070)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 3070)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth
Biol (2016) In press"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(11-OCT-2016) School of Life Sciences, University of Science and
Technology of China, University of Science and Technology of China,
No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China,
Hefei, Anhui 230026, China"
COMMENT SGRef: number: 3; type: "Journal Article"
COMMENT ##Assembly-Data-START##
Assembly Method :: Vector NTI v. Vector NTI Advance (TM) 11.0
Assembly Name :: GFPuv-reporter
Coverage :: 3070bp
Sequencing Technology :: 454
##Assembly-Data-END##
FEATURES Location/Qualifiers
source 1..3070
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 28..39
/regulatory_class="ribosome_binding_site"
RBS 28..39
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 46..759
/label=GFPuv
/note="GFP variant optimized for excitation by UV light"
terminator 825..872
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1066..1804
/label=CloDF13 ori
/note="Plasmids containing the CloDF13 (CDF) origin of
replication can be propagated in E. coli cells that contain
additional plasmids with compatible origins."
CDS complement(1967..2824)
/label=AmpR
/note="beta-lactamase"
promoter complement(2825..2929)
/label=AmpR promoter
terminator complement(3019..3062)
/label=bacterial terminator
/note="putative bacterial transcription terminator"
This page is informational only.