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GFPuv-reporter vector (V009957#)

Basic Vector Information

Basic Vector Information
Vector Name GFPuv-reporter Antibiotic Resistance Ampicillin
Length 3070 bp Type Expression vector
Source Han T, Chen Q, Liu H.

GFPuv-reporter vector Vector Map

GFPuv-reporter3070 bp6001200180024003000GFPuvAmpRAmpR promoterbacterial terminator

Plasmid Resuspension protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

GFPuv-reporter vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3070 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector GFPuv-reporter, complete sequence.
ACCESSION   KX980038
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium 
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27794600
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of 
            Science and Technology of China, University of Science and 
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0 
            Assembly Name         :: GFPuv-reporter
            Coverage              :: 3070bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /organism="Expression vector GFPuv-reporter"
                     /mol_type="other DNA"
                     /db_xref="taxon:1920042"
     regulatory      1..20
                     /regulatory_class="promoter"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      28..39
                     /regulatory_class="ribosome_binding_site"
     RBS             28..39
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             46..762
                     /codon_start=1
                     /product="GFPuv"
                     /label=GFPuv
                     /protein_id="APD28479.1"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     regulatory      769..897
                     /regulatory_class="terminator"
     terminator      825..872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1066..1804
     CDS             complement(1964..2824)
                     /codon_start=1
                     /product="AmpR"
                     /label=AmpR
                     /protein_id="APD28480.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2825..2929)
                     /gene="bla"
                     /label=AmpR promoter
     terminator      3019..3062
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"

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