LOCUS       40924_1064        3070 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector GFPuv-reporter, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3070)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Engineered photoactivatable genetic switches based on the bacterium 
            phage T7 RNA polymerase
  JOURNAL   ACS Synth Biol (2016) In press
  PUBMED    27794600
REFERENCE   2  (bases 1 to 3070)
  AUTHORS   Han T, Chen Q, Liu H.
  TITLE     Direct Submission
  JOURNAL   Submitted (11-OCT-2016) School of Life Sciences, University of 
            Science and Technology of China, University of Science and 
            Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui,
            230026,P.R.China, Hefei, Anhui 230026, China
REFERENCE   3  (bases 1 to 3070)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol (2016) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (11-OCT-2016) School of Life Sciences, University of Science and 
            Technology of China, University of Science and Technology of China, 
            No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, 
            Hefei, Anhui 230026, China"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: Vector NTI v. Vector NTI Advance (TM) 11.0 
            Assembly Name         :: GFPuv-reporter
            Coverage              :: 3070bp
            Sequencing Technology :: 454
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      28..39
                     /regulatory_class="ribosome_binding_site"
     RBS             28..39
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             46..759
                     /label=GFPuv
                     /note="GFP variant optimized for excitation by UV light"
     terminator      825..872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1066..1804
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(1967..2824)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2825..2929)
                     /label=AmpR promoter
     terminator      complement(3019..3062)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
ORIGIN
        1 taatacgact cactataggg actagtgaaa gaggagaaat actagatgag taaaggagaa
       61 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      121 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      181 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct
      241 tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag
      301 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac
      361 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta
      421 aaaggtattg attttaaaga agatggaaac attctcggac acaaactcga gtacaactat
      481 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc
      541 aaaattcgcc acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat
      601 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct
      661 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact
      721 gctgctggga ttacacatgg catggatgag ctctacaaat aatctagact gctaacaaag
      781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
      841 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatctg
      901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgatca aaggatcttc
     1081 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg
     1141 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt
     1201 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca
     1261 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc
     1321 tgccagtggc gttttaccgt gcttttccgg gttggactca agtgaacagt taccggataa
     1381 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac
     1441 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgtaagg
     1501 gagaaaggcg gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg
     1561 agcgacccgc cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca
     1621 gattcatggt tgagcctcac ggctcccaca gatgcaccgg aaaagcgtct gtttatgtga
     1681 actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct gctgttttgc
     1741 ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta tgtgtccgca
     1801 gcgcgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     1861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     1921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     1981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     2041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     2101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     2161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     2221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     2281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     2341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     2401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     2461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     2521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     2581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     2641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     2701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     2761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     2821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     2881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     2941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
     3001 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
     3061 tctggaattc
//
