LOCUS 40924_1064 3070 bp DNA circular SYN 17-DEC-2018 DEFINITION Expression vector GFPuv-reporter, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3070) AUTHORS Han T, Chen Q, Liu H. TITLE Engineered photoactivatable genetic switches based on the bacterium phage T7 RNA polymerase JOURNAL ACS Synth Biol (2016) In press PUBMED 27794600 REFERENCE 2 (bases 1 to 3070) AUTHORS Han T, Chen Q, Liu H. TITLE Direct Submission JOURNAL Submitted (11-OCT-2016) School of Life Sciences, University of Science and Technology of China, University of Science and Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, Hefei, Anhui 230026, China REFERENCE 3 (bases 1 to 3070) TITLE Direct Submission REFERENCE 4 (bases 1 to 3070) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth Biol (2016) In press" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted (11-OCT-2016) School of Life Sciences, University of Science and Technology of China, University of Science and Technology of China, No.96, JinZhai Road Baohe District,Hefei,Anhui, 230026,P.R.China, Hefei, Anhui 230026, China" COMMENT SGRef: number: 3; type: "Journal Article" COMMENT ##Assembly-Data-START## Assembly Method :: Vector NTI v. Vector NTI Advance (TM) 11.0 Assembly Name :: GFPuv-reporter Coverage :: 3070bp Sequencing Technology :: 454 ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..3070 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 1..19 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" regulatory 28..39 /regulatory_class="ribosome_binding_site" RBS 28..39 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 46..759 /label=GFPuv /note="GFP variant optimized for excitation by UV light" terminator 825..872 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin 1066..1804 /label=CloDF13 ori /note="Plasmids containing the CloDF13 (CDF) origin of replication can be propagated in E. coli cells that contain additional plasmids with compatible origins." CDS complement(1967..2824) /label=AmpR /note="beta-lactamase" promoter complement(2825..2929) /label=AmpR promoter terminator complement(3019..3062) /label=bacterial terminator /note="putative bacterial transcription terminator" ORIGIN 1 taatacgact cactataggg actagtgaaa gaggagaaat actagatgag taaaggagaa 61 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac 121 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa 181 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttctct 241 tatggtgttc aatgcttttc ccgttatccg gatcatatga aacggcatga ctttttcaag 301 agtgccatgc ccgaaggtta tgtacaggaa cgcactatat ctttcaaaga tgacgggaac 361 tacaagacgc gtgctgaagt caagtttgaa ggtgataccc ttgttaatcg tatcgagtta 421 aaaggtattg attttaaaga agatggaaac attctcggac acaaactcga gtacaactat 481 aactcacaca atgtatacat cacggcagac aaacaaaaga atggaatcaa agctaacttc 541 aaaattcgcc acaacattga agatggatcc gttcaactag cagaccatta tcaacaaaat 601 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc gacacaatct 661 gccctttcga aagatcccaa cgaaaagcgt gaccacatgg tccttcttga gtttgtaact 721 gctgctggga ttacacatgg catggatgag ctctacaaat aatctagact gctaacaaag 781 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 841 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatctg 901 caggcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 961 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 1021 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgatca aaggatcttc 1081 ttgagatcct ttttttctgc gcgtaatctt ttgccctgta aacgaaaaaa ccacctgggg 1141 aggtggtttg atcgaaggtt aagtcagttg gggaactgct taaccgtggt aactggcttt 1201 cgcagagcac agcaaccaaa tctgtccttc cagtgtagcc ggactttggc gcacacttca 1261 agagcaaccg cgtgtttagc taaacaaatc ctctgcgaac tcccagttac caatggctgc 1321 tgccagtggc gttttaccgt gcttttccgg gttggactca agtgaacagt taccggataa 1381 ggcgcagcag tcgggctgaa cggggagttc ttgcttacag cccagcttgg agcgaacgac 1441 ctacaccgag ccgagatacc agtgtgtgag ctatgagaaa gcgccacact tcccgtaagg 1501 gagaaaggcg gaacaggtat ccggtaaacg gcagggtcgg aacaggagag cgcaagaggg 1561 agcgacccgc cggaaacggt ggggatcttt aagtcctgtc gggtttcgcc cgtactgtca 1621 gattcatggt tgagcctcac ggctcccaca gatgcaccgg aaaagcgtct gtttatgtga 1681 actctggcag gagggcggag cctatggaaa aacgccaccg gcgcggccct gctgttttgc 1741 ctcacatgtt agtcccctgc ttatccacgg aatctgtggg taactttgta tgtgtccgca 1801 gcgcgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 1921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 2041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 2101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 2161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 2221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 2281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 2341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 2401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 2461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 2521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 2581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 2641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 2701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 2761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 2821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 2881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 2941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 3001 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 3061 tctggaattc //