FOXP3 vector (V009983)

LOCUS       V009983                 9841 bp    DNA     circular SYN 17-DEC-2018
DEFINITION  Exported.
ACCESSION   V009983
VERSION     V009983
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Hematopoietic Stem Cell Gene Therapy for IPEX Syndrome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2018) Microbiology, Immunology and Molecular
            Genetics, University of California, Los Angeles, 610 Charles E.
            Young Dr. East, Los Angeles, CA 90095, USA
REFERENCE   3  (bases 1 to 9841)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-OCT-2018) Microbiology, Immunology and Molecular Genetics,
            University of California, Los Angeles, 610 Charles E. Young Dr.
            East, Los Angeles, CA 90095, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label="AmpR promoter"
     CDS             132..989
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      1163..1751
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    2039..2060
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        2174..2192
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             2891..3071
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2891..2986
                     /label="R region LTR"
                     /note="R region LTR"
     misc_feature    2897..3071
                     /label="U5 region LTR"
                     /note="U5 region LTR"
     misc_feature    3118..3243
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3736..3969
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             4152..4196
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             4345..4386
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    4489..4606
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     regulatory      4655..4892
                     /label="CNS1"
                     /note="CNS1"
                     /regulatory_class="enhancer"
     regulatory      4893..5252
                     /label="CNS2"
                     /note="CNS2"
                     /regulatory_class="enhancer"
     regulatory      5252..5470
                     /label="CNS3"
                     /note="CNS3"
                     /regulatory_class="enhancer"
     regulatory      5471..5853
                     /label="FOXP3"
                     /note="FOXP3"
                     /regulatory_class="promoter"
     5'UTR           5854..6041
     CDS             6042..7334
                     /gene="FOXP3"
                     /label="Forkhead box protein P3"
                     /note="Forkhead box protein P3 from Homo sapiens.
                     Accession#: Q9BZS1"
     3'UTR           7338..8213
     misc_feature    8291..8331
                     /label="U3 region LTR (first half)"
                     /note="U3 region LTR (first half)"
     misc_feature    8332..8597
                     /label="A2 insulator"
                     /note="A2 insulator"
     misc_feature    8598..8614
                     /label="U3 region LTR (second half)"
                     /note="U3 region LTR (second half)"
     LTR             8615..8795
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8867..9000
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      9027..9162
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(9195..9213)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9223..9239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      9384..9839
                     /direction=RIGHT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"