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FOXP3 vector (V009983#)

Basic Vector Information

Basic Vector Information
Vector Name FOXP3 Antibiotic Resistance Ampicillin
Length 9841 bp Type Expression vector
Source Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.

FOXP3 vector Vector Map

AmpR promoter AmpR CMV CMV promoter 5' LTR (truncated) HIV-1 Psi RRE cPPT/CTS CNS1 CNS2 CNS3 FOXP3 5'UTR FOXP3 3'UTR U3 region LTR (first half) A2 insulator U3 region LTR (second half) 5' LTR (truncated) SV40 poly(A) signal SV40 ori T7 promoter M13 fwd f1 ori

FOXP3 vector Sequence

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LOCUS       Exported                9841 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector FOXP3, complete sequence.
ACCESSION   MK012431
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Hematopoietic Stem Cell Gene Therapy for IPEX Syndrome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2018) Microbiology, Immunology and Molecular 
            Genetics, University of California, Los Angeles, 610 Charles E. 
            Young Dr. East, Los Angeles, CA 90095, USA
REFERENCE   3  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Expression vector FOXP3"
                     /mol_type="other DNA"
                     /db_xref="taxon:2492469"
     promoter        27..131
                     /gene="bla"
                     /label=AmpR promoter
     CDS             132..992
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            132..989
                     /gene="ampicillin resistance"
                     /label=ampicillin resistance
     regulatory      complement(1009..2361)
                     /regulatory_class="promoter"
                     /note="CMV"
     rep_origin      1163..1751
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2891..2986
                     /label=R region LTR
                     /note="R region LTR"
     misc_feature    2897..3071
                     /label=U5 region LTR
                     /note="U5 region LTR"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    4489..4606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      4655..4892
                     /regulatory_class="enhancer"
                     /note="CNS1"
     regulatory      4893..5252
                     /regulatory_class="enhancer"
                     /note="CNS2"
     regulatory      5252..5470
                     /regulatory_class="enhancer"
                     /note="CNS3"
     regulatory      5471..5853
                     /regulatory_class="promoter"
                     /note="FOXP3"
     5'UTR           5854..6041
     gene            6042..7334
                     /gene="FOXP3"
                     /label=FOXP3
     3'UTR           7338..8213
     misc_feature    8291..8331
                     /label=U3 region LTR (first half)
                     /note="U3 region LTR (first half)"
     misc_feature    8332..8597
                     /label=A2 insulator
                     /note="A2 insulator"
     misc_feature    8598..8614
                     /label=U3 region LTR (second half)
                     /note="U3 region LTR (second half)"
     LTR             8615..8795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8615..8710
                     /label=R region LTR
                     /note="R region LTR"
     misc_feature    8711..8794
                     /label=U5 region LTR
                     /note="U5 region LTR"
     polyA_signal    8867..8987
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9027..9162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9195..9213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9223..9239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9384..9839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"

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