LOCUS       40924_860        9841 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector FOXP3, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Hematopoietic Stem Cell Gene Therapy for IPEX Syndrome
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9841)
  AUTHORS   Masiuk KE, Laborada J, Grazia Roncarolo M, Hollis RP, Kohn DB.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-OCT-2018) Microbiology, Immunology and Molecular 
            Genetics, University of California, Los Angeles, 610 Charles E. 
            Young Dr. East, Los Angeles, CA 90095, USA
REFERENCE   3  (bases 1 to 9841)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9841)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-OCT-2018) Microbiology, Immunology and Molecular Genetics, 
            University of California, Los Angeles, 610 Charles E. Young Dr. 
            East, Los Angeles, CA 90095, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        27..131
                     /label=AmpR promoter
     CDS             132..989
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1163..1751
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2039..2060
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2075..2105
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2113..2129
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2137..2153
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2174..2192
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        2293..2672
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2673..2876
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             2891..3071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2891..2986
                     /label=R region LTR
                     /note="R region LTR"
     misc_feature    2897..3071
                     /label=U5 region LTR
                     /note="U5 region LTR"
     misc_feature    3118..3243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    3736..3969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4152..4196
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             4345..4386
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    4489..4606
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     regulatory      4655..4892
                     /label=CNS1
                     /note="CNS1"
                     /regulatory_class="enhancer"
     regulatory      4893..5252
                     /label=CNS2
                     /note="CNS2"
                     /regulatory_class="enhancer"
     regulatory      5252..5470
                     /label=CNS3
                     /note="CNS3"
                     /regulatory_class="enhancer"
     regulatory      5471..5853
                     /label=FOXP3
                     /note="FOXP3"
                     /regulatory_class="promoter"
     5'UTR           5854..6041
     CDS             6042..7334
                     /gene="FOXP3"
                     /label=FOXP3
                     /note="Forkhead box protein P3 from Homo sapiens.
                     Accession#: Q9BZS1"
     3'UTR           7338..8213
     misc_feature    8291..8331
                     /label=U3 region LTR (first half)
                     /note="U3 region LTR (first half)"
     misc_feature    8332..8597
                     /label=A2 insulator
                     /note="A2 insulator"
     misc_feature    8598..8614
                     /label=U3 region LTR (second half)
                     /note="U3 region LTR (second half)"
     LTR             8615..8795
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8867..9000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      9027..9162
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(9195..9213)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9223..9239)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      9384..9839
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
      121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
      181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
      241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
      301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
      361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
      421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
      481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
      541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
      601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
      661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
      721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
      781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
      841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
      901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
      961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     2161 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttggc
     2221 cattgcatac gttgtatcca tatcataata tgtacattta tattggctca tgtccaacat
     2281 taccgccatg ttgacattga ttattgacta gttattaata gtaatcaatt acggggtcat
     2341 tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat ggcccgcctg
     2401 gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa
     2461 cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact
     2521 tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta
     2581 aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt
     2641 acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg
     2701 ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg
     2761 ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc
     2821 cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt
     2881 tagtgaaccg gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     2941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     3001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     3061 aatctctagc agtggcgccc gaacagggac ctgaaagcga aagggaaacc agaggagctc
     3121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     3181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     3241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     3301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     3361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     3421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     3481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     3541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     3601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     3661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     3721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     3781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     3841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     3901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     3961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     4021 gaatgctagt tggagtaata aatctctgga acagatggaa tcacacgacc tggatggagt
     4081 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4141 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4201 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4261 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4321 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4381 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4441 gatctcgacg gtatcgatct cgacacaaat ggcagtattc atccacaatt ttaaaagaaa
     4501 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
     4561 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
     4621 ggacagcaga gatccagttt gggtcgagga tatctaggtt agtctttttt tctgtggctt
     4681 ctgtctctgg ttttgtgctt agaaagtcct ttcctacttg aaaatgagat aaatgttcac
     4741 ctatgttggc ttctagtctc ttttatggct tcattttttc catttactat agaggttaag
     4801 agtgtgggta ctggagccag actgtctggg acaaacccag cgtcacccca agccctatgt
     4861 gtgattttta gccaggcact taacctctcc atccaagaag ggccaggtct tcagagctag
     4921 gggcttgtca tagtggccag atggacatca cctaccacat ccaccagcac ccatgtcacc
     4981 ccacctgggc caagcctgct gcaggacagg gcagccagtt ctcggaacga aacctgtggg
     5041 gtggggtatc tgccctcttc tcttcctccg tggtgtcgat gaagcccggc gcatccggcc
     5101 gccatgacgt caatggcgga aaaatctggg caagtcgggg gctgtgacaa cagggcccag
     5161 atgcagaccc cgatatgaaa acataatctg tgtcccagaa acatccccca ttcagcttct
     5221 gagaaaccca gtcagaaagg gacgtcccaa cagaggccct gggcccagga tggggcaggc
     5281 agggtggggt acctggacct acaggtgccg acctttactg tggcactggg cgggaggggg
     5341 gctggctggg gcacaggaag tggtttctgg gtcccaggca agtctgtgac ttatgcagat
     5401 gttgcagggc caagaaaatc cccacctgcc aggcctcaga gattggaggc tctccccgac
     5461 ctcccaatcc tcccatccac acatagagct tcagattctc tttctttccc cagagaccct
     5521 caaatatcct ctcactcaca gaatggtgtc tctgcctgcc tcgggttggc cctgtgattt
     5581 attttagttc ttttcccttg tttttttttt ttcaaactct atacactttt gttttaaaaa
     5641 ctgtggtttc tcatgagccc tattatctca ttgatacctc tcacctctgt ggtgagggga
     5701 agaaatcata ttttcagatg actcgtaaag ggcaaagaaa aaaacccaaa atttcaaaat
     5761 ttccgtttaa gtctcataat caagaaaagg agaaacacag agagagagaa aaaaaaaact
     5821 atgagaaccc ctccccaccc cgtgattatc agcgcacaca ctcatcgaaa aaaatttgga
     5881 ttattagaag agagaggtct gcggcttcca caccgtacag cgtggttttt cttctcggta
     5941 taaaagcaaa gttgtttttg atacgtgaca gtttcccaca agccaggctg atccttttct
     6001 gtcagtccac ttcaccaagc ctgcccttgg acaaggaccc gatgcccaac cccaggcctg
     6061 gcaagccctc ggccccttcc ttggcccttg gcccatcccc aggagcctcg cccagctgga
     6121 gggctgcacc caaagcctca gacctgctgg gggcccgggg cccaggggga accttccagg
     6181 gccgagatct tcgaggcggg gcccatgcct cctcttcttc cttgaacccc atgccaccat
     6241 cgcagctgca gctgcccaca ctgcccctag tcatggtggc accctccggg gcacggctgg
     6301 gccccttgcc ccacttacag gcactcctcc aggacaggcc acatttcatg caccagctct
     6361 caacggtgga tgcccacgcc cggacccctg tgctgcaggt gcaccccctg gagagcccag
     6421 ccatgatcag cctcacacca cccaccaccg ccactggggt cttctccctc aaggcccggc
     6481 ctggcctccc acctgggatc aacgtggcca gcctggaatg ggtgtccagg gagccggcac
     6541 tgctctgcac cttcccaaat cccagtgcac ccaggaagga cagcaccctt tcggctgtgc
     6601 cccagagctc ctacccactg ctggcaaatg gtgtctgcaa gtggcccgga tgtgagaagg
     6661 tcttcgaaga gccagaggac ttcctcaagc actgccaggc ggaccatctt ctggatgaga
     6721 agggcagggc acaatgtctc ctccagagag agatggtaca gtctctggag cagcagctgg
     6781 tgctggagaa ggagaagctg agtgccatgc aggcccacct ggctgggaaa atggcactga
     6841 ccaaggcttc atctgtggca tcatccgaca agggctcctg ctgcatcgta gctgctggca
     6901 gccaaggccc tgtcgtccca gcctggtctg gcccccggga ggcccctgac agcctgtttg
     6961 ctgtccggag gcacctgtgg ggtagccatg gaaacagcac attcccagag ttcctccaca
     7021 acatggacta cttcaagttc cacaacatgc gacccccttt cacctacgcc acgctcatcc
     7081 gctgggccat cctggaggct ccagagaagc agcggacact caatgagatc taccactggt
     7141 tcacacgcat gtttgccttc ttcagaaacc atcctgccac ctggaagaac gccatccgcc
     7201 acaacctgag tctgcacaag tgctttgtgc gggtggagag cgagaagggg gctgtgtgga
     7261 ccgtggatga gctggagttc cgcaagaaac ggagccagag gcccagcagg tgttccaacc
     7321 ctacacctgg cccctgatga cctcaagatc aaggaaagga ggatggacga acaggggcca
     7381 aactggtggg aggcagaggt ggtgggggca gggatgatag gccctggatg tgcccacagg
     7441 gaccaagaag tgaggtttcc actgtcttgc ctgccagggc ccctgttccc ccgctggcag
     7501 ccaccccctc ccccatcata tcctttgccc caaggctgct cagaggggcc ccggtcctgg
     7561 ccccagcccc cacctccgcc ccagacacac cccccagtcg agccctgcag ccaaacagag
     7621 ccttcacaac cagccacaca gagcctgcct cagctgctcg cacagattac ttcagggctg
     7681 gaaaagtcac acagacacac aaaatgtcac aatcctgtcc ctcactcaac acaaacccca
     7741 aaacacagag agcctgcctc agtacactca aacaacctca aagctgcatc atcacacaat
     7801 cacacacaag cacagccctg acaacccaca caccccaagg cacgcaccca cagccagcct
     7861 cagggcccac aggggcactg tcaacacagg ggtgtgccca gaggcctaca cagaagcagc
     7921 gtcagtaccc tcaggatctg aggtcccaac acgtgctcgc tcacacacac ggcctgttag
     7981 aattcacctg tgtatctcac gcatatgcac acgcacagcc ccccagtggg tctcttgagt
     8041 cccgtgcaga cacacacagc cacacacact gccttgccaa aaataccccg tgtctcccct
     8101 gccactcacc tcactcccat tccctgagcc ctgatccatg cctcagctta gactgcagag
     8161 gaactactca tttatttggg atccaaggcc cccaacccac agtaccgtcc ccgaattcga
     8221 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     8281 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat cagagcgaga
     8341 ttccgtctca aagaaaaaaa aagtaatgaa atgaataaaa tgagtcctag agccagtaaa
     8401 tgtcgtaaat gtctcagcta gtcaggtagt aaaaggtctc aactaggcag tggcagagca
     8461 ggattcaaat tcagggctgt tgtgatgcct ccgcagactc tgagcgccac ctggtggtaa
     8521 tttgtctgtg cctcttctga cgtggaagaa cagcaactaa cacactaaca cggcatttac
     8581 tatgggccag ccattgttgc tttttgcttg tactgggtct ctctggttag accagatctg
     8641 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8701 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     8761 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     8821 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     8881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     8941 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     9001 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     9061 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     9121 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga
     9181 cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca
     9241 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     9301 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     9361 cagcctgaat ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     9421 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     9481 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     9541 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     9601 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     9661 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     9721 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     9781 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc
     9841 c
//
