FBDki vector (V009987)

LOCUS       40924_810       10504 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Knock-in vector FBDki, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Tamayev R, Giliberto L, Matsuda S, D'Abramo C, Arancio O, Vidal R, 
            D'Adamio L.
  TITLE     Memory deficits due to Familial British Dementia BRI2 mutation are 
            caused by loss of BRI2 function rather than amyloidosis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Giliberto L, D'Adamio L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) Litwin-Zucker Center for the Study of 
            Alzheimer's Disease, The Feinstein Institute for Medical 
            Research-North Shore-LIJ, 350 Community Drive, Manhasset, NY 11030, 
            USA
REFERENCE   3  (bases 1 to 10504)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2010) Litwin-Zucker Center for the Study of Alzheimer's 
            Disease, The Feinstein Institute for Medical Research-North 
            Shore-LIJ, 350 Community Drive, Manhasset, NY 11030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2257..2481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          complement(2555..2620)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2695..3267)
                     /label=DTA
                     /note="diphtheria toxin fragment A"
     regulatory      complement(3286..3797)
                     /label=PGK1 promoter
                     /note="PGK1 promoter"
                     /regulatory_class="promoter"
     promoter        complement(3294..3788)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    3828..4989
                     /note="left arm; contains intron 5"
     misc_feature    5038..5072
                     /label=LoxP
                     /note="LoxP"
     protein_bind    5038..5071
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    complement(5130..5354)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(5395..6195)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(6209..6703)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    complement(6733..6766)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6780..6796)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6805..6825
                     /label=pBlueScript MCS fragment
                     /note="pBlueScript MCS fragment"
     primer_bind     6805..6821
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6829..9835
                     /note="right arm fragment; contains intron 5, exon 6, and
                     intron 6"
     exon            7037..7156
                     /number=6
     CDS             7037..7156
                     /codon_start=1
                     /product="ITM2B integral membrane protein 2B"
                     /label=ITM2B integral membrane protein 2B
                     /note="ABRI"
                     /protein_id="ADO40090.1"
                     /translation="GIQKREASNCFAIRHFENKFAVETLICSRTVKKNIIEEN"
     misc_difference 7070
                     /replace="a"
                     /label=humanized
                     /note="humanized"
     misc_feature    7121..7156
                     /label=ABRI mutant-extra bases
                     /note="ABRI mutant-extra bases"
     misc_difference 7121
                     /replace="t"
                     /label=ABRI mutation
                     /note="ABRI mutation"
     3'UTR           7157..7960
                     /label=ITM2B 3' UTR
                     /note="ITM2B 3' UTR"
     promoter        complement(9861..9879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9889..9905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10047..10502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"