LOCUS       40924_810       10504 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Knock-in vector FBDki, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10504)
  AUTHORS   Tamayev R, Giliberto L, Matsuda S, D'Abramo C, Arancio O, Vidal R, 
            D'Adamio L.
  TITLE     Memory deficits due to Familial British Dementia BRI2 mutation are 
            caused by loss of BRI2 function rather than amyloidosis
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10504)
  AUTHORS   Giliberto L, D'Adamio L.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAY-2010) Litwin-Zucker Center for the Study of 
            Alzheimer's Disease, The Feinstein Institute for Medical 
            Research-North Shore-LIJ, 350 Community Drive, Manhasset, NY 11030, 
            USA
REFERENCE   3  (bases 1 to 10504)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10504)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (24-MAY-2010) Litwin-Zucker Center for the Study of Alzheimer's 
            Disease, The Feinstein Institute for Medical Research-North 
            Shore-LIJ, 350 Community Drive, Manhasset, NY 11030, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10504
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        24..128
                     /label=AmpR promoter
     CDS             129..986
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1160..1748
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2036..2057
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2072..2102
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2110..2126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2134..2150
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2171..2189
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    2257..2481
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          complement(2555..2620)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(2695..3267)
                     /label=DTA
                     /note="diphtheria toxin fragment A"
     regulatory      complement(3286..3797)
                     /label=PGK1 promoter
                     /note="PGK1 promoter"
                     /regulatory_class="promoter"
     promoter        complement(3294..3788)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_feature    3828..4989
                     /note="left arm; contains intron 5"
     misc_feature    5038..5072
                     /label=LoxP
                     /note="LoxP"
     protein_bind    5038..5071
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     polyA_signal    complement(5130..5354)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     CDS             complement(5395..6195)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     promoter        complement(6209..6703)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     protein_bind    complement(6733..6766)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6780..6796)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6805..6825
                     /label=pBlueScript MCS fragment
                     /note="pBlueScript MCS fragment"
     primer_bind     6805..6821
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    6829..9835
                     /note="right arm fragment; contains intron 5, exon 6, and
                     intron 6"
     exon            7037..7156
                     /number=6
     CDS             7037..7156
                     /codon_start=1
                     /product="ITM2B integral membrane protein 2B"
                     /label=ITM2B integral membrane protein 2B
                     /note="ABRI"
                     /protein_id="ADO40090.1"
                     /translation="GIQKREASNCFAIRHFENKFAVETLICSRTVKKNIIEEN"
     misc_difference 7070
                     /replace="a"
                     /label=humanized
                     /note="humanized"
     misc_feature    7121..7156
                     /label=ABRI mutant-extra bases
                     /note="ABRI mutant-extra bases"
     misc_difference 7121
                     /replace="t"
                     /label=ABRI mutation
                     /note="ABRI mutation"
     3'UTR           7157..7960
                     /label=ITM2B 3' UTR
                     /note="ITM2B 3' UTR"
     promoter        complement(9861..9879)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9889..9905)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      10047..10502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
       61 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      121 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      181 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      241 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      301 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      361 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
      421 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
      481 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
      541 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
      601 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
      661 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
      721 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
      781 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
      841 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
      901 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
      961 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     1021 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     1081 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     1141 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     1201 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     1261 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     1321 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     1381 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     1441 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     1501 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     1561 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     1621 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     1681 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     1741 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     1801 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     1861 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     1921 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     1981 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     2041 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     2101 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     2161 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct gggtaccggg ccccccctcg
     2221 aggtcgagcc ccagctggtt ctttccgcct cagaagccat agagcccacc gcatccccag
     2281 catgcctgct attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca
     2341 gaatagaatg acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga
     2401 cagtgggagt ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg
     2461 gctggcaact agaaggcaca gtcgaggctg atcagcgagc tctaggatct gcattccacc
     2521 actgctccca ttcatcagtt ccataggttg gaatctaaaa tacacaaaca attagaatca
     2581 gtagtttaac acattataca cttaaaaatt ttatatttac cttagagctt taaatctctg
     2641 taggtagttt gtccaattat gtcacaccac agaagtaagg ttccttcaca aagatcgcct
     2701 gacacgattt cctgcacagg cttgagccat atactcatac atcgcatctt ggccacgttt
     2761 tccacgggtt tcaaaattaa tctcaagttt tacgcttaac gctttcgcct gttcccagtt
     2821 attaatatat tcgacgctag aactcccctc ggcaaaggga aggctgagca ctacacgcga
     2881 agcaccatcc ccgaaccttt tgataaactc ttctgttccg acttgctcca tcaacggttc
     2941 agtgagactt aaacctaact ctttcttaat agtttcggca ttatccactt ttagtgcgag
     3001 gaccttcgtc agtcctggat acgtcacttt gaccacgtct ccagcttttc cagagagcgg
     3061 gttttcgtta tctacagagt atcccgcagc gtcgtattta ttgtcggtac tataaaaccc
     3121 tttccaatca tcgtcataat ttccttgtgt accagatttt ggcttttgta tacctttttg
     3181 aatggaatct acataaccag gtttagtccc gtggtacgaa gaaaagtttt ccatcacaaa
     3241 agatttagaa gaatcaacaa catcatcagg atccatggcg aggacctgca ggtcgaaagg
     3301 cccggagatg aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat
     3361 gccgggctcc ggaggacctt cgcgcccgcc ccgcccctga gcccgcccct gagcccgccc
     3421 ccggacccac cccttcccag cctctgagcc cagaaagcga aggagccaag ctgctattgg
     3481 ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga
     3541 ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg
     3601 gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaaggga
     3661 gccggttggc gctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc
     3721 gccaagtgcc agcggggctg ctaaagcgca tgctccagac tgccttggga aaagcgcctc
     3781 ccctacccgg tagaattcga tatcaagctt atcgataccg tcaattccca tgacgcttgg
     3841 gtgacaatgt aaagggaccc tgtgtcatgc ttggttagtt tttccaagat ttagaatcat
     3901 ggaaatccca aaccctctct cttcagatag aaaccatcag tcagcaggaa atgccattgc
     3961 tttgggacgc acacccaccc ctcagtttgc atgtagaaga gtcaggtttg acttttcatc
     4021 ctcactgtgg ctgggccaca ggagagagga ggtctttcag cctcacgctt ggactcttag
     4081 ggaatctgag ttctcacagg tcccgtcacc tgtggatcgt gctgaggcta gtcacagcca
     4141 ggtgacttag gaagacctgt aggtgaggag tctggagccc tgacatgacc tcggcagctc
     4201 tgctgtcact aaatcttgtt ttccccctga gcagtaagaa tagcagcatg tgcccactat
     4261 aaatgcaacg cagaagtgtg tgtgacctta cacatcgtcc agtgccatcc gtcggtgacg
     4321 ccacagcctg gcttgtgtgg gattgtgtca cccagcagaa gtgcttcttt tctggtcttt
     4381 gccaatactt gtagtgacat tgttctcacc tcaggctgct gagtcatctg aaacctacca
     4441 cttgatttct tacatttaag tcacgatctt gcctcataat tttctaaaac caatttgata
     4501 gaacgaagtt tgtctctccc ggtgtgcagt tctgcaagca cacacgggca tgtgactcca
     4561 ccgtatgtaa ctagaatacc tcccaaacct tggagctcat gcatctgtgt cttagggttt
     4621 ctgtggctgc aacaaaacac catggccaaa aaaaagcaag ttggagagga aagggtttat
     4681 tcagcttata cttccacatt gctgttcatc accaaaggag gtcaggacag gaactcaaac
     4741 aaagcaggaa cctggaggca ggagcaggtg cagggaccat gaaggagtgc tgtttactgt
     4801 ccccatggct tgctcagcct gctttcttat agaacccagg accaccagcc cagggatggc
     4861 accacccaca ataggctggg ccctcccaca ccaatcacta attaagaaga tgctctacag
     4921 gtgaatctta tgaaggcatt ttctgaatta aggttccttc cttgcagatg actctagttt
     4981 gtgtatcaag ttgacggtat cgataaagct agcttgggct gcaggtcgag ggacctaata
     5041 acttcgtata gcatacatta tacgaagtta tattaagggt tccggatcag cttgattcga
     5101 gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc cagcatgcct
     5161 gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     5221 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     5281 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     5341 actagaaggc acagtcgagg ctgatcagcg agctctagag aattgatccc ctcagaagaa
     5401 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     5461 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     5521 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     5581 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     5641 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     5701 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     5761 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     5821 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag
     5881 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     5941 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     6001 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     6061 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     6121 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     6181 aatggccgat cccatattgg ctgcaggtcg aaaggcccgg agatgaggaa gaggagaaca
     6241 gcgcggcaga cgtgcgcttt tgaagcgtgc agaatgccgg gctccggagg accttcgcgc
     6301 ccgccccgcc cctgagcccg cccctgagcc cgcccccgga cccacccctt cccagcctct
     6361 gagcccagaa agcgaaggag ccaagctgct attggccgct gccccaaagg cctacccgct
     6421 tccattgctc agcggtgctg tccatctgca cgagactagt gagacgtgct acttccattt
     6481 gtcacgtcct gcacgacgcg agctgcgggg cgggggggaa cttcctgact aggggaggag
     6541 tagaaggtgg cgcgaagggg ccaccaaaga agggagccgg ttggcgctac cggtggatgt
     6601 ggaatgtgtg cgaggccaga ggccacttgt gtagcgccaa gtgccagcgg ggctgctaaa
     6661 gcgcatgctc cagactgcct tgggaaaagc gcctccccta cccggtagaa ttcctgcagg
     6721 tcgagggacc taataacttc gtatagcata cattatacga agttatatta agggttccgg
     6781 atccactagt tctagagcgg ccgctctaga actagtggat cccccgacta ggcagcacat
     6841 ctgtctgtaa gccgtttgta aagcactgtt accagttgct aggatatatg gaaaggccca
     6901 gtggagcaca aatttcaaat tatgggatgg atatagagta gtggcatgct tctctatgtc
     6961 taaagagtaa ctgtgagtat ttgttactaa acaacttaaa acaaaaccaa aaccagcccc
     7021 ctttcctttc ccaacaggta ttcagaagcg ggaagccagt aactgtttcg ccattcggca
     7081 ttttgagaac aaatttgctg tggagacttt aatttgttct agaacagtca agaaaaacat
     7141 tattgaggaa aattgaattc aatgccacag cataccctgc ccctttgtat tttgtgcagt
     7201 gattgttttt taaaatcttc ttttcatgta agtagcaaac agggcttcac tgtctcttca
     7261 tctcaataac tcaattaaaa accattatct taaaaaaaga aaacaaaacc tttctttttt
     7321 ctaagtgtgg tgtctttgat gtttgaatta gcaaatgtgc aggttcctag ataagattcg
     7381 cttctcctta gagcttacct actaggaaga atctaaattg cttggaaatc actaatctgg
     7441 atttttgtgt taattctgca cttccatgag ggaaagatgc ctaaagaata gtcattcgca
     7501 tatgttaaag ggaccacagt gacttgcttg tagatgctag ccctgctacc tagtctgtta
     7561 gcatttgaag tcaccttctc atactacttt aattaaaatg tgccgtatct tcaatgttgc
     7621 tttaactact ttagaggatt tcagccttga tgttttaata tcctaggcct ctgctgtaat
     7681 aagattttag acaaatgttt ggaatttaag aagcaactca tgttactaat ttgtatagcc
     7741 catatctgtg gaatggaata taaatatcac aaagccatgt gatgagactg tgcgttgttt
     7801 ttcccatagg ataaaaccaa agaagtaatt tggttcttca tactttaagg taatccacat
     7861 acataaaaaa tgaaactatt ttataaagtc tagttctcta catgcagtta taaaaatcag
     7921 ctttttaaaa aaataaaata agccattaat tactaaatca gatttcttgt ggatgctgtt
     7981 tcagtgacac cgagggcaaa cttgaccaac gctgcttgac aggaagtggg ccttgtcatt
     8041 cagtggatgg ttagcattga agctaagatg tggatctaag tggtggttct cagtctgttc
     8101 tggtttttct tcagactcat ctcactgtgc tgttgtaact ggtagtctct agggaccagc
     8161 ctctccagca gggagataga catgtcccac agaacctggc aagctagaga tgggattttt
     8221 gttgctgttt cagtttttga aactgggtct cagtctggaa actaggctga cttggaaatc
     8281 aggccagtct caaactcgtg atgattctct gcctcagctt ccagggtgtt aagatcacta
     8341 tcatagcaac cacacctgag gttgcttctt agctgagaga attcagaatc cactgcctgg
     8401 aaaacacctg agagtaggtc tggagcaatg gctcagaagt cacgggtgct tattgctggt
     8461 ctggaggacc aagctcagtt cccagcaccc actttgggtg gctcacaact gcctgtaaat
     8521 gcaggtctgg gcacctaaca ccctcttctg gcctccactg gcctctgcac acaatgtaac
     8581 atacctgtga ataaaaagaa gtgacactaa gagcagtttt gctataagtt tctgaaacaa
     8641 aatatttcaa ttttgacttt aggttggaat tttttaaaac ttcaagagtc cacaaagaat
     8701 ttggtttgga gagttcagaa ggcagttttg tgcggcctgc ctctcagttt tatttcagta
     8761 attctgccag agctttagtg cactaaacgg atggttaagg aatgagaact taccctagaa
     8821 gagaggattt actaaagtga caactacatt ttcctacctc ccaggcttta aaatttcatg
     8881 gcttttcagg ggaggggcct gtaaacattc aagagtgaat agtgccccat attgggccaa
     8941 cacatacaga tacgtgtaga gatagattcc actcaaaaaa aaaaagtgtt gcgccttata
     9001 ttataatcca gtaagtgagt aatgagtact gagttacagt ttgttatgat aattactgga
     9061 cagaccttca tctaaacatt tttatgatct gtaaatcaaa gcaccttgaa ggtgctagac
     9121 aaatggcagt cactaagata gagccccatc ctccttatac accgccgtca ggctgtcatt
     9181 tgggaattcc tgggcattcc gtaaggtttg tagttgaagg aagggtgttg gtctgttcct
     9241 gcatttatag tagtgcactg tcagggtggc aagcagaggc aacaacccca gcttctcctc
     9301 actgacttca cacagtatga gtttgcctgc cgtcagcacg gggtgggggg gggtggctcc
     9361 agcatcatta gaatggttta agaatttcat acggtcccag ccaaacactt gatatcgcag
     9421 aacatttaca ctatcggcta gatcagacta gcaggttgtc tctgacttta tttccattac
     9481 tattatgtca gtcgagtaga ttgccccaag tcaggagtgg tggtgtgcac ctgtaatctc
     9541 tgcacttgag aaactgaaac cggatgatca tgaggtcaag acaatgtaaa cagcatgggg
     9601 ttccaggtga ctttcaacca catgctgaga tctttaaggc cagaaagggg ggggggggga
     9661 aagcgctctc acccacactt ggccaggtgt ctccctccca ggaatttgag aatccctagc
     9721 tgtggttctg gcaggtacca tttgatctca ggttctgtga tggagccttg aataagctag
     9781 agagtgaagg gccgctggtt tccagtttgc aagggggctg ctgtccttcc tggacctcga
     9841 gcggtggagc tccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     9901 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     9961 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
    10021 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
    10081 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
    10141 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
    10201 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
    10261 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
    10321 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
    10381 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
    10441 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
    10501 ttag
//
