Basic Vector Information
EXP5(+) vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
EXP5(+) vector Sequence
LOCUS Exported 6761 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Cloning vector EXP5(+), complete sequence.
ACCESSION EF028664
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6761)
AUTHORS Meima ME, Weening KE, Schaap P.
TITLE Vectors for expression of proteins with single or combinatorial
fluorescent protein and tandem affinity purification tags in
Dictyostelium
JOURNAL Protein Expr. Purif. 53 (2), 283-288 (2007)
PUBMED 17296313
REFERENCE 2 (bases 1 to 6761)
AUTHORS Meima ME, Weening KE, Schaap P.
TITLE Direct Submission
JOURNAL Submitted (27-SEP-2006) School of Life Sciences, University of
Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE 3 (bases 1 to 6761)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..6761
/organism="Cloning vector EXP5(+)"
/lab_host="Dictyostelium discoideum"
/mol_type="other DNA"
/note="Cloning vector EXP5(+) was generated from Cloning
vector EXP4(+) by inserting a longer multiple cloning site"
/db_xref="taxon:409537"
misc_feature 15..59
/label=multipe cloning site
/note="multipe cloning site"
regulatory 71..1051
/regulatory_class="terminator"
/note="2H3 terminator"
CDS 1784..2614
/codon_start=1
/product="neomycin phosphotransferase"
/label=neomycin phosphotransferase
/protein_id="ABK40469.1"
/translation="MDGEDVQAGSFRMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSD
AAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWL
LLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAG
LVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCG
RLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 1820..2614
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
promoter 3504..3608
/gene="bla"
/label=AmpR promoter
CDS 3609..4469
/codon_start=1
/product="beta-lactamase"
/label=beta-lactamase
/protein_id="ABK40468.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4640..5228
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
regulatory 6453..6761
/regulatory_class="promoter"
/note="actin15 promoter"
This page is informational only.