LOCUS       40924_805        6761 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EXP5(+), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6761)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Vectors for expression of proteins with single or combinatorial 
            fluorescent protein and tandem affinity purification tags in 
            Dictyostelium
  JOURNAL   Protein Expr. Purif. 53 (2), 283-288 (2007)
  PUBMED    17296313
REFERENCE   2  (bases 1 to 6761)
  AUTHORS   Meima ME, Weening KE, Schaap P.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2006) School of Life Sciences, University of 
            Dundee, Dow Street, Dundee DD1 5EH, United Kingdom
REFERENCE   3  (bases 1 to 6761)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6761)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Expr. Purif."; date: "2007"; volume: "53"; issue: "2"; pages: 
            "283-288"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (27-SEP-2006) School of Life Sciences, University of Dundee, Dow 
            Street, Dundee DD1 5EH, United Kingdom"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    15..59
                     /label=multipe cloning site
                     /note="multipe cloning site"
     regulatory      71..1051
                     /label=2H3 terminator
                     /note="2H3 terminator"
                     /regulatory_class="terminator"
     CDS             1820..2611
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        3504..3608
                     /label=AmpR promoter
     CDS             3609..4466
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4640..5228
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     regulatory      6453..6761
                     /label=actin15 promoter
                     /note="actin15 promoter"
                     /regulatory_class="promoter"
ORIGIN
        1 agatctaaaa aatgggatcc aagcttacta gttctagaga attcatcgat taactcgagg
       61 atctgctaga ggtaccacaa gtggtggtgg tggtatgggt tcatttggtg gtcgatccga
      121 tgaagaatca agtgatccaa atgctatact tggtctcttt gaagacgata ttttctgggg
      181 tgataatgat gaatacttta gtgataacta ccgttatgtt gatgaagaag atattgatga
      241 agatttcaat ggtaatgatg aatttgaaat tgatgttgaa ggtgatttcc aagaagatga
      301 tttcgaagaa gaatatgcat tttattaaat taaacttttt ttcataaaaa aataataata
      361 ataatttaat aactaatcat tatcatcaat aataacatta taatattact ttaataacaa
      421 tacttttttt taatattcaa agactttaca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      481 caaaccataa acaaaactaa aaataaatta aaaaaaaaaa taaaaaaaaa aaaaaaaaaa
      541 aaaactaaaa atacaattat tttattattt aaataaataa ttgtattata atttgtattt
      601 ttgtgttttt gtttttttta tttctttaaa atgatttata acttttattt tttataaagg
      661 aaaataacat tttccttaaa tgataaaaga tcatttaaaa catttgtatt aaaaggttca
      721 ttaccattat ataaatcatt aaataattgt ttttgaactg tgaaaacgaa tttagtatgt
      781 ttcaaatcag tgatttgtgg attatcaact aatattgatt gaattgttgg tgtatcatca
      841 tgagcataag aaccatcatc cattatactt tcaacatgaa aatattttgt aattttttta
      901 ttgattgatg aaatatatct aatgagttga tcagaattta aacctaatat tgataatttt
      961 cttaacggtg caattgatga aatcattgaa ccaaactctt taacgaatgc attggataac
     1021 tttatcgatc tttgttttct ttgagagaat tagcggccgc taattcagag gtttattttt
     1081 aaaaattaca aaaactaaaa agaaaaataa aaaggaaaaa tcttattaat ctgaaaatta
     1141 cagatttgcc ccaatttaaa gaaaaattat ccaaaataat caatcaacaa cgatatcttt
     1201 ttgatagata tttataaaaa ctttatcttt tttatttttt caaagttgcg caaaataata
     1261 aaattaaaat aaatataaaa actgtaaaaa gaaaaaaaaa agtgtaaagg tttattaatt
     1321 atttaattat tattcacttt ttgtaattat ttttttattt tgaagaataa tgatggatat
     1381 tttatataaa aaaaaaaaga gatatgaaaa aataataatt ataaaaaaaa aaaaaaaaaa
     1441 tagaatttta aagttttagt acaaatggat gatttttttt tttttttttt ttttttccta
     1501 aataattcaa gtaataaaca aagaattgat attctgatgc ctaattaaaa aagaaatttt
     1561 taaataaaaa atgggttttt tttaagtaaa gttatttgaa attgattgaa attttcaaac
     1621 catgggtggt ttttcgcttt aaaattggga ttttattttt atttttttat attttttatt
     1681 ttttattttt tttttttttg aggtttctga gattataaaa tgaaattttt tttttttttt
     1741 taattaattc aaaaaataat caaataaata aatataatat aaaatggacg gagaagatgt
     1801 tcaagctgga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     1861 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     1921 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     1981 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     2041 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     2101 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     2161 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     2221 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     2281 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     2341 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     2401 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     2461 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     2521 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     2581 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     2641 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     2701 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     2761 ctcatgctgg agttcttcgc ccaccccggg ctcgatcccc tcgcgagttg gttcagctgc
     2821 tgcctgaggc tggacgacct cgcggagttc taccggcagt gcaaatccgt cggcatccag
     2881 gaaaccagca gcggctatcc gcgcatccat gcccccgaac tgcaggagtg gggaggcacg
     2941 atggccgctt tggtcccgga tcagctttat ctttttgatt ttcatactct ttggctgttt
     3001 tacatctaat gcatatattt atcttcacca ttcaaaatgt atctgtaaat aataaatttt
     3061 ttaaatatat ttgatttttt tgatagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     3121 attaaaataa cttaataatt attaagattt aaaaaaaaaa aattaaaaaa aaaagaaaat
     3181 attagattat tacaacaatt tgttttttta ttatttatta aataatttag aaacattttc
     3241 tgtgaacaat tgatggaccc tcaggaagat cgcactccag ccagctttcc ggcaccgctt
     3301 ctggtgccgg aaaccaggca aagcgccatt cgccattcag gctgcgcaac tgttgggaag
     3361 ggcgatcggt ggcggggatc agcggccgct atcagatcaa ttcttgaaga cgaaagggcc
     3421 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     3481 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     3541 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     3601 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     3661 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     3721 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     3781 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     3841 tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     3901 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     3961 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     4021 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     4081 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     4141 ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     4201 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     4261 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     4321 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     4381 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     4441 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     4501 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     4561 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     4621 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     4681 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     4741 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     4801 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     4861 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     4921 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     4981 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     5041 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     5101 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     5161 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     5221 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     5281 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     5341 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     5401 aagcggaaga gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc
     5461 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     5521 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     5581 aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc
     5641 cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc
     5701 gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta
     5761 gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca
     5821 acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg
     5881 tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc
     5941 tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg
     6001 acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg
     6061 ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg
     6121 ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga
     6181 aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg
     6241 cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta
     6301 cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc
     6361 accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgagca gctgaagcta
     6421 gcttgcatgc ctgcaggtcg actctagcta gataaaaaaa atttttattt atttttattt
     6481 attttgaatt aaatagatta caaattaatt aatcccatca aatctttaaa aaaaaaaatg
     6541 gtttaaaaaa acttgggttg gttaattatt atttgaaaat tttaaaaccc aaattaaaaa
     6601 aaaaaaatgg gattcaaaaa tttttttttt tttttttttt tttttttttt tttttttttt
     6661 tttttttttt ttttttttca gattgcataa aaagattttt tttttttttt ttttcttatt
     6721 tcttaaaaca aataaattaa attaaataaa aaataaaaat c
//
