eif1a-dsRed-eif1a-sfGFP-tVE1 vector (V010001)

Basic Vector Information

      • Vector Name:
      • eif1a-dsRed-eif1a-sfGFP-tVE1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 9494 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.

eif1a-dsRed-eif1a-sfGFP-tVE1 vector Vector Map

eif1a-dsRed-eif1a-sfGFP-tVE19494 bp400800120016002000240028003200360040004400480052005600600064006800720076008000840088009200M13 fwdeif1alphaloxPvertebrate consensus sequence for strong initiation of translation (Kozak, 1987)SV40 poly(A) signalloxPeif1alpha5'UTRvertebrate consensus sequence for strong initiation of translation (Kozak, 1987)SV40 poly(A) signalattB3M13 fwdSV40 poly(A) signalSP6 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

eif1a-dsRed-eif1a-sfGFP-tVE1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                9494 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector eif1a-dsRed-eif1a-sfGFP-tVE1, complete sequence.
ACCESSION   KU645306
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, 
            Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 9494)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9494
                     /organism="Cloning vector eif1a-dsRed-eif1a-sfGFP-tVE1"
                     /mol_type="other DNA"
                     /label=derived from Danio rerio
                     /note="derived from Danio rerio"
                     /db_xref="taxon:1891372"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     regulatory      438..1597
                     /regulatory_class="promoter"
                     /note="eif1alpha"
     intron          959..1531
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    1600..1633
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      1640..1649
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1646..2323
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     CDS             1679..2323
                     /codon_start=1
                     /product="DsRed"
                     /label=DsRed
                     /note="red fluorescent protein"
                     /protein_id="AOE47047.1"
                     /translation="MRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVKLKVTKGGPLPF
                     AWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVATVTQDSSLQD
                     GCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGETHKALKLKDGGHYL
                     VEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHHLFL"
     polyA_signal    2444..2565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    2819..2852
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     regulatory      2900..4059
                     /regulatory_class="promoter"
                     /note="eif1alpha"
     intron          3421..3993
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     5'UTR           4068..4110
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     regulatory      4135..4144
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4141..5763
                     /codon_start=1
                     /product="SfGFP/Aqp3aR220Q fusion protein"
                     /label=SfGFP/Aqp3aR220Q fusion protein
                     /note="tVE1 allele"
                     /protein_id="AOE47048.1"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKSGLRSMGWQKSVLDKLAQTFQIRNKLLRQGLAECLGTLILVM
                     FGCGSLAQLKLSEGSHGLFLTANLAFGFGATLGILVCGQVSGGHLNPAVTFALCLLGRE
                     KWRKFPVYFLFQTLGSFLGAAIIFAEYHDAIYDYAGESNELLVLGEKETAGIFATYPSK
                     YLTPLNGFFDQVIGTASLIVCILAIVDPYNNPIPQGLEAFTVGFSVLIIGLSMGFNSGY
                     AVNPAQDFGPRLFTAMAGWGSEVFTARDYWFLVPIFAPFIGAVIGVIVYQLMVGWHVEG
                     EARDKKAKAREEVMNLNDVASKE"
     polyA_signal    5796..5930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5993..6013)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6021..6037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6053..6187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6769..6787)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6805..6821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6829..6845
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6853..6883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6898..6919
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7207..7795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7966..8826)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8827..8931)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(9009..9464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"

This page is informational only.