LOCUS       40924_760        9494 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector eif1a-dsRed-eif1a-sfGFP-tVE1, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9494)
  AUTHORS   Eskova A, Garcia-Junco R, Chauvigne F, Maischein H-M., Ammelburg M, 
            Cerda J, Kaderali L, Nusslein-Volhard C, Irion U.
  TITLE     Gain-of-function mutations of mau/DrAqp3a influence zebrafish 
            pigment pattern formation through the tissue environment
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9494)
  AUTHORS   Eskova A.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-JAN-2016) e-CNV group, Max Planck Institute for 
            Developmental Biology, Spemannstr. 35-39, Tuebingen 72076, Germany
REFERENCE   3  (bases 1 to 9494)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9494)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (28-JAN-2016) e-CNV group, Max Planck Institute for Developmental 
            Biology, Spemannstr. 35-39, Tuebingen 72076, Germany"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     111..127
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          959..1531
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    1600..1633
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     CDS             1646..2320
                     /label=DsRed2
                     /note="improved tetrameric variant of DsRed fluorescent
                     protein"
     polyA_signal    2444..2565
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(2819..2852)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2900..4059
                     /label=eif1alpha
                     /note="eif1alpha"
                     /regulatory_class="promoter"
     intron          3421..3993
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     5'UTR           4068..4110
                     /label=Xenopus globin 5'-UTR
                     /note="translational enhancer from the 5'-UTR of the major 
                     beta-globin gene of Xenopus laevis"
     CDS             4141..4857
                     /label=superfolder GFP
                     /note="GFP variant that folds robustly even when fused to
                     poorly folded proteins (Pedelacq et al., 2006)"
     polyA_signal    5796..5930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5993..6013)
                     /label=attB3
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     primer_bind     complement(6021..6037)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(6053..6187)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(6769..6787)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6805..6821)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6829..6845)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6853..6883)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6898..6919)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7207..7795)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7969..8826)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8827..8931)
                     /label=AmpR promoter
     rep_origin      complement(9009..9464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
ORIGIN
        1 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
       61 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      121 ggccagtgaa ttgtaatacg actcmctata gggcgaattg ggcccagagg tgtaaagtac
      181 ttgagtaatt ttacttgatt actgtactta agtattattt ttggggattt ttactttact
      241 tgagtacaat taaaaatcaa tacttttact tttacttaat tacatttttt tagaaaaaaa
      301 agtacttttt actccttaca attttattta cagtcaaaaa gtacttattt tttggagatc
      361 acttcattct attttccctt gctattacca aaccaattga attgcgctga tgcccagttt
      421 aattttcggt acaaatatcg agcaggggga tcatctaatc aagcacaaat aaggggcgtg
      481 taacacaaaa gccagcgacc ctttccaatg caaatcaaac ttgcaattct ttgccgtttt
      541 tatcatttaa gtgtcggctt aaggtccact atcagatgta aacagcctta tctaacaaag
      601 gtatcattac attctgaaat tctcaggcat gcaagctagc ttatgacgca ctagggagtg
      661 ccacccttcc tttcgcccta acttcgtgat aactcgcgcg tttcactcaa cagctgcatc
      721 cgccctagtg ctactgggag ttgtagtata caagacgctt acaggctgaa tgttctgtca
      781 agaccccgcc tctagcactt tgggaattct ggacttgatg atgtcatggt taatccccgc
      841 ccagtagagg cggctatata aagggtggtt aaggcccggt tcgctctctt cctcaccggg
      901 tctgcggcga gttctagctg aagcttcctg caggtcgacc gatcctgaga acttcagggt
      961 gagtttgggg acccttgatt gttctttctt tttcgctatt gtaaaattca tgttatatgg
     1021 agggggcaaa gttttcaggg tgttgtttag aatgggaaga tgtcccttgt atcaccatgg
     1081 accctcatga taattttgtt tctttcactt tctactctgt tgacaaccat tgtctcctct
     1141 tattttcttt tcattttctg taactttttc gttaaacttt agcttgcatt tgtaacgaat
     1201 ttttaaattc acttttgttt atttgtcaga ttgtaagtac tttctctaat cacttttttt
     1261 tcaaggcaat cagggtatat tatattgtac ttcagcacag ttttagagaa caattgttat
     1321 aattaaatga taaggtagaa tatttctgca tataaattct ggctggcgtg gaaatattct
     1381 tattggtaga aacaactaca ccctggtcat catcctgcct ttctctttat ggttacaatg
     1441 atatacactg tttgagatga ggataaaata ctctgagtcc aaaccgggcc cctctgctaa
     1501 ccatgttcat gccttcttct ctttcctaca gctcctgggc aacgtgctgg ttgttgtgct
     1561 gtctcatcat tttggcaaag aattcctcga cggccggcca taacttcgta tagcatacat
     1621 tatacgaagt tatccggtcg ccaccatggc ctcctccgag aacgtcatca ccgagttcat
     1681 gcgcttcaag gtgcgcatgg agggcaccgt gaacggccac gagttcgaga tcgagggcga
     1741 gggcgagggc cgcccctacg agggccacaa caccgtgaag ctgaaggtga ccaagggcgg
     1801 ccccctgccc ttcgcctggg acatcctgtc cccccagttc cagtacggct ccaaggtgta
     1861 cgtgaagcac cccgccgaca tccccgacta caagaagctg tccttccccg agggcttcaa
     1921 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggcg accgtgaccc aggactcctc
     1981 cctgcaggac ggctgcttca tctacaaggt gaagttcatc ggcgtgaact tcccctccga
     2041 cggccccgtg atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc
     2101 ccgcgacggc gtgctgaagg gcgagaccca caaggccctg aagctgaagg acggcggcca
     2161 ctacctggtg gagttcaagt ccatctacat ggccaagaag cccgtgcagc tgcccggcta
     2221 ctactacgtg gacgccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga
     2281 gcagtacgag cgcaccgagg gccgccacca cctgttcctg tagcggccgc gactctagat
     2341 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     2401 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     2461 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     2521 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggcg taaattgtaa
     2581 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2641 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2701 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2761 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagat
     2821 aacttcgtat agcatacatt atacgaagtt atgatctggc catctagagc ggccgcgcgc
     2881 actagtgaat tccatggatt cgagcagggg gatcatctaa tcaagcacaa ataaggggcg
     2941 tgtaacacaa aagccagcga ccctttccaa tgcaaatcaa acttgcaatt ctttgccgtt
     3001 tttatcattt aagtgtcggc ttaaggtcca ctatcagatg taaacagcct tatctaacaa
     3061 aggtatcatt acattctgaa attctcaggc atgcaagcta gcttatgacg cactagggag
     3121 tgccaccctt cctttcgccc taacttcgtg ataactcgcg cgtttcactc aacagctgca
     3181 tccgccctag tgctactggg agttgtagta tacaagacgc ttacaggctg aatgttctgt
     3241 caagaccccg cctctagcac tttgggaatt ctggacttga tgatgtcatg gttaatcccc
     3301 gcccagtaga ggcggctata taaagggtgg ttaaggcccg gttcgctctc ttcctcaccg
     3361 ggtctgcggc gagttctagc tgaagcttcc tgcaggtcga ccgatcctga gaacttcagg
     3421 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat
     3481 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat
     3541 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct
     3601 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga
     3661 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt
     3721 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt
     3781 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt
     3841 cttattggta gaaacaacta caccctggtc atcatcctgc ctttctcttt atggttacaa
     3901 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct
     3961 aaccatgttc atgccttctt ctctttccta cagctcctgg gcaacgtgct ggttgttgtg
     4021 ctgtctcatc attttggcaa agaattcctc gacggccggc cagcttgctt gttctttttg
     4081 cagaagctca gaataaacgc tcaactttgg cagatcaatt ccccccaccc tagcgccacc
     4141 atggtgtcta aaggagagga gctgttcaca ggcgtggtgc caatcctggt ggagctggat
     4201 ggagacgtga acggccacaa gttcagcgtg agaggcgagg gagagggaga cgccacaaac
     4261 ggcaagctga cactgaaatt catctgcaca acaggcaaac tgcctgtgcc ttggccaacc
     4321 ctggtgacaa ccctgacata cggagtgcag tgctttagca gataccctga tcacatgaaa
     4381 cagcacgatt tcttcaagag cgccatgcct gagggctacg tgcaggagag aaccatcagc
     4441 ttcaaggatg acggaaccta caagacaaga gccgaggtga agtttgaggg agatacactg
     4501 gtgaacagaa tcgagctgaa aggcatcgat ttcaaagagg atggcaacat cctgggacac
     4561 aaactggagt acaacttcaa cagccacaac gtgtacatca cagccgataa acagaagaac
     4621 ggcatcaaag ccaacttcaa gatcagacac aatgtggagg atggatctgt gcagctggcc
     4681 gatcactacc agcagaacac acctatcgga gacggcccag tgctgctgcc agataaccac
     4741 tacctgagca cacagagcgt gctgtctaaa gaccctaacg agaagagaga tcacatggtg
     4801 ctgctggagt ttgtgacagc cgccggaatc accctgggca tggatgagct gtacaaatcc
     4861 ggactcagat ctatgggttg gcagaaaagc gttctggata agcttgcgca gactttccaa
     4921 atccgcaaca agttactgcg ccagggattg gctgaatgct taggaactct catcctggtg
     4981 atgtttggct gtggttcatt ggcccagttg aaactaagcg aaggttctca tggtctcttt
     5041 ctcactgcaa accttgcttt tgggtttggt gctactcttg gaatcttggt ttgcggccag
     5101 gtgtcaggcg gacatttaaa tcctgctgtt acatttgctc tctgcctctt gggaagagaa
     5161 aaatggagaa agtttcctgt gtactttctg ttccaaacac tcggatcctt cttgggtgct
     5221 gctattatct ttgccgaata ccatgacgca atttatgatt atgctggaga atcaaatgag
     5281 ttgcttgtac tgggtgaaaa agaaacagct gggatttttg ctacataccc aagcaaatat
     5341 ctcacccccc taaatggatt ttttgaccag gtgataggca cagcatccct gattgtgtgc
     5401 atcctggcca ttgtggaccc ctacaacaac ccgatccctc aaggtcttga ggccttcaca
     5461 gtgggattca gcgtccttat cattggtctc tccatgggct tcaattctgg ctatgcagta
     5521 aacccagctc aagattttgg acctcgtctt tttactgcca tggctggttg gggtagtgaa
     5581 gtcttcacag ccagggatta ttggtttttg gtgcccatct ttgctccgtt cattggagcc
     5641 gttattggtg tgattgtgta ccagctgatg gtgggatggc atgtggaagg agaggcacga
     5701 gataagaaag ctaaagctag agaggaggtg atgaacctca atgacgtcgc cagcaaggaa
     5761 tgagaattct gcagtcgacg gtaccgcggg cccgggatcc agacatgata agatacattg
     5821 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     5881 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     5941 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt tccaacttta
     6001 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
     6061 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     6121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     6181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     6241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
     6301 tcagatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg
     6361 ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt
     6421 ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac
     6481 gtcgccagca aggaatgaga attctgcagt cgaatcagct taaacaagaa tctctagttt
     6541 tctttcttgc ttttactttt acttccttaa tactcaagta caattttaat ggagtacttt
     6601 tttactttta ctcaagtaag attctagcca gatactttta cttttaattg agtaaaattt
     6661 tccctaagta cttgtacttt cacttgagta aaatttttga gtacttttta cacctctgct
     6721 cgaccatatg ggagagctcc caacgcgttg gatgcatagc ttgagtattc tatagtgtca
     6781 cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6841 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6901 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6961 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     7021 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     7081 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     7141 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7201 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7261 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7321 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7381 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7441 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7501 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7561 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7621 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     7681 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7741 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7801 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7861 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7921 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7981 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     8041 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     8101 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     8161 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8221 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8281 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8341 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8401 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8461 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8521 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8581 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8641 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8701 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8761 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8821 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8881 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8941 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     9001 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     9061 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     9121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     9181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     9241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     9301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     9361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     9421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     9481 ctgcgcaact gttg
//
