EF-PDGF vector (V010003)

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LOCUS       40924_715       11831 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EF-PDGF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 11831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(558..578)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(708..773)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    1555..1833
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             4458..5132
                     /codon_start=1
                     /label=DsRed-Express2
                     /note="noncytotoxic tetrameric variant of DsRed fluorescent
                     protein (Strack et al., 2008)"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     protein_bind    6627..6726
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     promoter        complement(7767..7797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7812..7833)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8883..9740)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9741..9845)
                     /label=AmpR promoter
     primer_bind     10319..10335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(join(11830..11831,1..133))
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"