LOCUS       40924_715       11831 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector EF-PDGF, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11831)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 11831)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 11831)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11831)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(558..578)
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     intron          complement(708..773)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     protein_bind    1555..1833
                     /label=tetracycline response element
                     /note="contains seven copies of the tetracycline operator
                     tetO"
     CDS             4458..5132
                     /codon_start=1
                     /label=DsRed-Express2
                     /note="noncytotoxic tetrameric variant of DsRed fluorescent
                     protein (Strack et al., 2008)"
                     /translation="MDSTENVIKPFMRFKVHMEGSVNGHEFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGTFIYHVKFIGVNFPSDGPVMQKKTLGWEPSTERLYPRDGVLKGEIHK
                     ALKLKGGGHYLVEFKSIYMAKKPVKLPGYYYVDSKLDITSHNEDYTVVEQYERAEARHH
                     LFQ"
     protein_bind    6627..6726
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     promoter        complement(7767..7797)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7812..7833)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8121..8709)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8883..9740)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9741..9845)
                     /label=AmpR promoter
     primer_bind     10319..10335
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(join(11830..11831,1..133))
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tcgagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
       61 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      121 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      181 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      241 ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat taaaaagcta
      301 aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct gtgtggagta
      361 agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt tacagaatat
      421 ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta aatagcaaag
      481 caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa ttctgaagga
      541 aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg ttgagagtca
      601 gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt ttcctcatta
      661 aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
      721 acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
      781 ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
      841 acaaagatcc aagcttaggt cacgggacgg gcggcagcaa cagtttcaca tttacaggcc
      901 aaatgatctt ccaaggtaac ggtggccttc ttgaaaatgg gctttttacg cacaatttca
      961 atcttacgaa cttgcacggg acgcaattga acttgggtgg gacgacattg cacattacga
     1021 ttattacaac aaccactaca acgttgcact tcaacacagg ggggccaaac caagaaattg
     1081 gcattggtac gatcaatcaa acgacgggaa atttcgaaca cttcggtacg ggttttacat
     1141 tcggcaatca tggcgggttc agcaatggtc aatgagccca atgaggcttg aacgaaggca
     1201 aaaacggcca agaaggcgaa caacaccaac aagaacgact tcatggttgt gtgactttgt
     1261 ttttcttctt tgttgtgtat ataaacttga tgaatgattt gtaatagttt ttctatttat
     1321 agaacttgtt gcttaaaact cactttttat agaattattt tactttcttt tgtacacttt
     1381 ctgatttatt taatttcttg ttcacttgaa cgtagttttt cttaatttaa atcgcttgtt
     1441 atttcttttt aatgttttca ctttgcttgt ttgtattgta ctaatgccga agagagtttg
     1501 tgcggccgta tttatatgaa tcacataaac cttggatccg ttcgagttta ccactcccta
     1561 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     1621 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttacgag
     1681 ctcggatctc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     1741 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     1801 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgaaac ctggcgcgcc
     1861 ccggccatcg agaaagagag agagaagaga agagagagaa cattcgagaa agagagagag
     1921 aagagaagag agagaacata cttccctatc agtgatagag aagtccctat cagtgataga
     1981 gatgtcccta tcagtgatag agagttccct atcagtgata gagacgtccc tatcagtgat
     2041 agagaagtcc ctatcagtga tagagagatc cctatcagtg atagagattt ccctatcagt
     2101 gatagagagg tccctatcag tgatagagac ttccctatca gtgatagaga aatccctatc
     2161 agtgatagag acatccctat cagtgataga gaactcccta tcagtgatag agacctccct
     2221 atcagtgata gagagaattc tgcggcccac gtgttgaaac cattatcaga aaataattca
     2281 cttaaacatt ataatttgat aaaaagaaca ctaaaacaaa atattataat tataccgcca
     2341 aaaattcaac aaactttaag aaatttcgag taaattgcac cgcaaactaa aaaaccgaca
     2401 tgataaacaa aaaaaataca taaaagagcg caaacagctg tcatttacag ggttgtagat
     2461 atttgaaatt tttacctaaa aaggggaaaa taaaaggcca cacttttgga aattacaaat
     2521 ttgtgagaga atttttgatt tcatttatta aatttatata aaaacaaaag agttgttata
     2581 atatgtatga gataatgttt gcaaatttta atttttaaat ttttacagtc aaattttata
     2641 aaatatgtta aaagtatatg attgtcacat ttttaaatta ttccactttt cattgttttt
     2701 attcaagacc ggcaactctg atgttttgct gcacatcagc tgcactggcg aaaaaattat
     2761 taatgccccc atgatgagag aaaatcaata ttttttcatt ttatgcattt ttataaattt
     2821 tgttagcaat attattgttt taataatgtt attattttct ttaataaaac aattaaatta
     2881 ctttcattta taatttatga tgactaatat gtaaatcgaa tgtgattaaa tcatacatac
     2941 tatttccata taaagcacaa agtttaatat ataattaata ttaaatttat ttaatttaaa
     3001 aattaatagt taatacatat ttcaattaat caaatacata ttaagcttgt aacttagttc
     3061 aaaaaacact taattataca tagatttgtc aattaataag actttttaac tataatttaa
     3121 agaataaaac gtttttgaaa taaataagtt cattgtttga ctagaaaaat atttagttta
     3181 tgtcatgata ttactcagca atgcctaaga ttaattcctt tttcatttaa ttaataactt
     3241 ttatatattt ttgcataaat aaataaagta aattatagtt gattcattcc gcatgttatt
     3301 aaaaacagag agaacattgt agaaattttg acattgatgc aattttacag tttaaaagcc
     3361 actgcttcta catatataca acgctgcgtg ggcattaatc atgttgaatg ttttgcacat
     3421 ttttttgata atttgcaact aataaattaa aataacaata aaaacctata attaatattg
     3481 gttttctaca attttaataa attaattttt ttatatatct aaaatgtaac caatttcttt
     3541 tgttatcacc aaataaacaa ccacatgatg ctgtttagag cctacggaag tacgggtagt
     3601 ttgttccaga acattctagt attttcgttt atttttgttt tacctataaa ttcagtgcat
     3661 ggctgctaac cggcatcagt cttgaaagaa aaattgtgca aaaaacacca agaattccaa
     3721 acgggagaaa gttaaaagtg ttttaatttt cttaataaag aaaagtctga aaaaagaaca
     3781 ttaaaaaagt gtgtagttgt ttaatttcaa ttgcaaaaag aaaaataatt taaaaagtat
     3841 ttatttttgt gaaattaata taaaacaaac acatcaaggc aagtttttat taaaaaaaat
     3901 aaaatgtagt aacaaaacgt ttttgttctt ttgaaaaaag tgtgatgtaa aaactcaggt
     3961 aatttgccag aaagaaaaac cttcaatatg gtttaagaat aacgttacta agtaattgta
     4021 aattgttcta gattaaattt tctttggcca tgacattttt agaaaataaa tctagtatat
     4081 aaagaaaatt tcaccatgct aataaaaaga aatttctagc atgcgatttt ttaaagaaaa
     4141 tttttcaaaa ttttttcgaa attataatcg atatttcatg ttttcattcg ttaaaaagca
     4201 tacccaatcc acccctaaaa aactagtaat tcttaactta aagagatttc tagattttta
     4261 ttaaaaattt tcagtaccaa tcccactccc tacacttaac gaaaaacgtg cgtcattaaa
     4321 attatttata cttcatattt tgattgatta ttttactaat aatttgtatt tttctatttt
     4381 ttttttatta aacacagatg cctgaagaag ttgaaacttt tgctttccaa gctgaaattg
     4441 ctcagcttaa aggatccatg gattctactg aaaatgttat caagcctttt atgcgtttta
     4501 aggtacacat ggaaggttct gtcaatggtc acgaatttga aatcgaaggt gaaggtgaag
     4561 gtaaacccta cgaaggtact caaactgcta aattgcaagt taccaagggt ggtcccttgc
     4621 cattcgcctg ggatattttg tccccacaat tccaatacgg ttctaaggta tacgttaagc
     4681 atcccgctga tattcccgat tataaaaagt tgtccttccc cgaaggtttt aagtgggaac
     4741 gtgtaatgaa tttcgaagat ggtggtgttg tcaccgttac tcaagattcc tctttgcaag
     4801 atggtacttt tatttaccac gtcaaattca tcggtgtaaa ctttccatca gatggtcctg
     4861 ttatgcaaaa gaaaaccttg ggttgggaac cttctactga acgtttgtat ccccgtgatg
     4921 gtgtcttgaa aggtgaaatt cataaggctt tgaaattgaa gggtggtggt cactacttgg
     4981 ttgaatttaa gtccatctac atggccaaaa agcccgttaa gttgccaggt tactactacg
     5041 tcgattcaaa attggatatt accagtcata atgaagatta caccgtagta gaacaatacg
     5101 aacgtgccga agcccgtcac cacttgtttc aataaaaaag cttgcagcca aacccatcat
     5161 cgacaaccat caaaaccaaa aaaaaaagac agaagaagtg tgtattgaaa aaattctcga
     5221 cctattagct gaaactactc aactaaacat tcaatatact tttttttaaa caaactgtta
     5281 ttttaaaaat gataacgaat ccaaaacaaa acacttgtaa atttttttta ttttcaaatt
     5341 tgaaaaaaac atgaatgaaa agaacaaatt ttaaagaaat gcactttaaa aacacgaaaa
     5401 aaaaacaaga acctaataaa atgaaaggct taagctgcat tgaatcataa tattccaaaa
     5461 gaaaattttt taatgtttta ctacttaatg gggggaaaaa gagggaaaac aactaaaaat
     5521 agtaattgtt aaaatgcttg gcaaaagttt aatgaacttg ccgaggtgta gaggaaacta
     5581 taaaaatgca tagtgagtgc attaaaaaca gcactagttt ggcctcattt tgatgggtac
     5641 aagaggaaga cgaacaattt taaaaataac tttcaagtgg aagatagatt tgcttgaaaa
     5701 cattttgaga atttactcta gctataaaga taaataacat tacttcckty wtwcccaaga
     5761 tttctttaaa ctatagatga ctcattaatg aatcgttgct ttttatttat gatttccctc
     5821 ccctcttaaa caagtacagt ttgttttcta tatacatacc tactcagagt tctatttttt
     5881 gttagcaaca gctctgccaa ctgcttgttg ccttttaata gagttttgtt gttgctgtgt
     5941 tggtcttaaa atcatatgaa gcattttatg tggtgggttt tataactatt tttgtttgga
     6001 tatagtattt ttgttttctg tactgtaact caacaacaca ttgcctttta tggtcatata
     6061 aatgagtaaa tatactatat tgtatctacc acgatgatct cattcacatt ttttagagtt
     6121 tacattggaa acaaatattt gaattttgta agaaaacact gcccaccaaa tttttaagtt
     6181 gtttatttgg atctttgtgt aaaagagggg ttttaagata aatttcaagt tcattttaat
     6241 gtatcatggg acgctaagat caaagaatat ttgttttaag taatgtaaag ttatattttc
     6301 tgcttgatca aatctattat aagatcttat atcacacagt gaattagttc ccaagaaagg
     6361 ggtaaagatg catatcattt agcttaaacg tttttcataa aactgaacgt catgggcaaa
     6421 ttttcattaa agtataattt tctccaaaga actggtctat tgtcctcact attttatatt
     6481 tccatagtac tggacaggaa cgcggtttaa ggatttgcgt ttttagttaa tataaatcaa
     6541 agtttttgac tacaccagcc cgcactgacg gacacaccga agccccggcg gcaaccctca
     6601 gcggatgccc cggggcttca cgttttccca ggtcagaagc ggttttcggg agtagtgccc
     6661 caactggggt aacctttgag ttctctcagt tgggggcgta gggtcgccga catgacacaa
     6721 ggggttgtga ccggggtgga cacgtacgcg ggtgcttacg accgtcagtc gcgcgagcgc
     6781 gactagtaca agccgaattc tgcagatatc catcacactg gcggccgcgt cgacccgcgg
     6841 ccatggaggc ctggtaccag atctgacaat gttcagtgca gagactcggc tacgcctcgt
     6901 ggactttgaa gttgaccaac aatgtttatt cttacctcta atagtcctct gtggcaaggt
     6961 caagattctg ttagaagcca atgaagaacc tggttgttca ataacatttt gttcgtctaa
     7021 tatttcacta ccgcttgacg ttggctgcac ttcatgtacc tcatctataa acgcttcttc
     7081 tgtatcgctc tggacgtcat cttcacttac gtgatctgat atttcactgt cagaatcctc
     7141 accaacaagc tcgtcatcgc tttgcagaag agcagagagg atatgctcat cgtctaaaga
     7201 actacccatt ttattatata ttagtcacga tatctataac aagaaaatat atatataata
     7261 agttatcacg taagtagaac atgaaataac aatataatta tcgtatgagt taaatcttaa
     7321 aagtcacgta aaagataatc atgcgtcatt ttgactcacg cggtcgttat agttcaaaat
     7381 cagtgacact taccgcattg acaagcacgc ctcacgggag ctccaagcgg cgactgagat
     7441 gtcctaaatg cacagcgacg gattcgcgct atttagaaag agagagcaat atttcaagaa
     7501 tgcatgcgtc aattttacgc agactatctt tctagggtta aaaaagattt gcgctttact
     7561 cgacctaaac tttaaacacg tcatagaatc ttcgtttgac aaaaaccaca ttgtggccaa
     7621 gctgtgtgac gcgacgcgcg ctaaagaatg gcaaaccaag tcgcgcgagc gtcgactcta
     7681 gaggatcccc gggtaccgag ctcgaattcg taatcatggt catagctgtt tcctgtgtga
     7741 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     7801 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     7861 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     7921 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7981 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     8041 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     8101 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     8161 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     8221 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     8281 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt
     8341 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     8401 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     8461 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     8521 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
     8581 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     8641 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     8701 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     8761 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     8821 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     8881 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     8941 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     9001 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     9061 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     9121 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     9181 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     9241 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     9301 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     9361 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     9421 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     9481 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     9541 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     9601 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     9661 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     9721 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     9781 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     9841 ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca
     9901 ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac
     9961 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
    10021 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10081 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10141 ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc
    10201 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    10261 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    10321 aaaacgacgg ccagtgccaa gctttgttta aaatataaca aaattgtgat cccacaaaat
    10381 gaagtggggc aaaatcaaat aattaatagt gtccgtaaac ttgttggtct tcaacttttt
    10441 gaggaacacg ttggacggca aatccgtgac tataacacaa gttgatttaa taattttagc
    10501 caacacgtcg ggctgcgtgt tttttgccga cgcgtctgtg tacacgttga ttaactggtc
    10561 gattaaactg ttgaaataat ttaatttttg gttcttcttt aaatctgtga tgaaattttt
    10621 taaaataact ttaaattctt cattggtaaa aaatgccacg ttttgcaact tgtgagggtc
    10681 taatatgagg tcaaactcag taggagtttt atccaaaaaa gaaaacatga ttacgtctgt
    10741 acacgaacgc gtattaacgc agagtgcaaa gtataagagg gttaaaaaat atattttacg
    10801 caccatatac gcatcgggtt gatatcgtta atatggatca atttgaacag ttgattaacg
    10861 tgtctctgct caagtctttg atcaaaacgc aaatcgacga aaatgtgtcg gacaatatca
    10921 agtcgatgag cgaaaaacta aaaaggctag aatacgacaa tctcacagac agcgttgaga
    10981 tatacggtat tcacgacagc aggctgaata ataaaaaaat tagaaactat tatttaaccc
    11041 tagaaagata atcatattgt gacgtacgtt aaagataatc atgcgtaaaa ttgacgcatg
    11101 tgttttatcg gtctgtatat cgaggtttat ttattaattt gaatagatat taagttttat
    11161 tatatttaca cttacatact aataataaat tcaacaaaca atttatttat gtttatttat
    11221 ttattaaaaa aaaacaaaaa ctcaaaattt cttctataaa gtaacaaaac ttttaaacat
    11281 tctctctttt acaaaaataa acttattttg tactttaaaa acagtcatgt tgtattataa
    11341 aataagtaat tagcttaact tatacataat agaaacaaat tatacttatt agtcagtcag
    11401 aaacaacttt ggcacatatc aatattatgc tctcgacaaa taactttttt gcattttttg
    11461 cacgatgcat ttgcctttcg ccttatttta gaggggcagt aagtacagta agtacgtttt
    11521 ttcattactg gctcttcagt actgtcatct gatgtaccag gcacttcatt tggcaaaata
    11581 ttagagatat tatcgcgcaa atatctcttc aaagtaggag cttctaaacg cttacgcata
    11641 aacgatgacg tcaggctcat gtaaaggttt ctcataaatt ttttgcgact ttggaccttt
    11701 tctcccttgc tactgacatt atggctgtat ataataaaag aatttatgca ggcaatgttt
    11761 atcattccgt acaataatgc cataggccac ctattcgtct tcctactgca gggtaaccac
    11821 cggttctaga c
//
