DR4 vector (V010007)

MSDS

Basic Vector Information

Vector Name:: DR4

Antibiotic Resistance:: Ampicillin

Length:: 14499 bp

Type:: Cloning vector

Replication origin:: ori

Source/Author:: Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, Scott MJ.

DR4 vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

DR4 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_680       14499 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DR4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 14499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1..133)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(558..578)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(708..773)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(861..1094)
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     CDS             complement(1251..1874)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      complement(1885..4895)
                     /label=Chhsp83
                     /note="Chhsp83"
                     /regulatory_class="promoter"
     regulatory      4896..7109
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             7110..7802
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      7812..9229
                     /label=Lctub pA
                     /note="Lctub pA"
                     /regulatory_class="polyA_signal_sequence"
     protein_bind    9295..9394
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     mobile_element  9527..10209
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
     primer_bind     complement(10387..10403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10411..10427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10435..10465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10480..10501)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10789..11377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11551..12408)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12409..12513)
                     /label=AmpR promoter
     primer_bind     12987..13003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  13702..14480
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC

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