DR4 vector (V010007)

LOCUS       40924_680       14499 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DR4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 14499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1..133)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(558..578)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(708..773)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(861..1094)
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     CDS             complement(1251..1874)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      complement(1885..4895)
                     /label=Chhsp83
                     /note="Chhsp83"
                     /regulatory_class="promoter"
     regulatory      4896..7109
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             7110..7802
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      7812..9229
                     /label=Lctub pA
                     /note="Lctub pA"
                     /regulatory_class="polyA_signal_sequence"
     protein_bind    9295..9394
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     mobile_element  9527..10209
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
     primer_bind     complement(10387..10403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10411..10427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10435..10465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10480..10501)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10789..11377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11551..12408)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12409..12513)
                     /label=AmpR promoter
     primer_bind     12987..13003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  13702..14480
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC