LOCUS       40924_680       14499 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector DR4, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Towards next generation maggot debridement therapy: transgenic 
            Lucilia sericata larvae that produce and secrete a human growth 
            factor
  JOURNAL   BMC Biotechnol. 16 (1), 30 (2016)
  PUBMED    27006073
REFERENCE   2  (bases 1 to 14499)
  AUTHORS   Linger RJ, Belikoff EJ, Yan Y, Li F, Wantuch HA, Fitzsimons HL, 
            Scott MJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2015) Entomology, North Carolina State University,
            112 Derieux Place, Raleigh, NC 27695-7614, USA
REFERENCE   3  (bases 1 to 14499)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14499)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016"; volume: "16"; issue: "1"; pages: "30"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-OCT-2015) Entomology, North Carolina State University, 112 
            Derieux Place, Raleigh, NC 27695-7614, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..14499
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    complement(1..133)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(558..578)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(708..773)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(861..1094)
                     /label=VP16 AD
                     /note="transcriptional activation domain of herpes simplex
                     virus protein VP16 (Triezenberg et al., 1988; Cousens et 
                     al., 1989)"
     CDS             complement(1251..1874)
                     /label=TetR
                     /note="tetracycline repressor TetR"
     regulatory      complement(1885..4895)
                     /label=Chhsp83
                     /note="Chhsp83"
                     /regulatory_class="promoter"
     regulatory      4896..7109
                     /label=Lchsp83
                     /note="Lchsp83"
                     /regulatory_class="promoter"
     CDS             7110..7802
                     /label=ZsGreen
                     /note="Zoanthus green fluorescent protein (Matz et al.,
                     1999)"
     regulatory      7812..9229
                     /label=Lctub pA
                     /note="Lctub pA"
                     /regulatory_class="polyA_signal_sequence"
     protein_bind    9295..9394
                     /label=phage phi-C31 attP
                     /note="attachment site of phage phi-C31"
     mobile_element  9527..10209
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
     primer_bind     complement(10387..10403)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10411..10427
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10435..10465)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(10480..10501)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(10789..11377)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11551..12408)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(12409..12513)
                     /label=AmpR promoter
     primer_bind     12987..13003
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     mobile_element  13702..14480
                     /mobile_element_type="transposon:pBAC"
                     /label=BAC
ORIGIN
        1 tcgagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
       61 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
      121 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
      181 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
      241 ctagtcaagg cactatacat caaatattcc ttattaaccc ctttacaaat taaaaagcta
      301 aaggtacaca atttttgagc atagttatta atagcagaca ctctatgcct gtgtggagta
      361 agaaaaaaca gtatgttatg attataactg ttatgcctac ttataaaggt tacagaatat
      421 ttttccataa ttttcttgta tagcagtgca gctttttcct ttgtggtgta aatagcaaag
      481 caagcaagag ttctattact aaacacagca tgactcaaaa aacttagcaa ttctgaagga
      541 aagtccttgg ggtcttctac ctttctcttc ttttttggag gagtagaatg ttgagagtca
      601 gcagtagcct catcatcact agatggcatt tcttctgagc aaaacaggtt ttcctcatta
      661 aaggcattcc accactgctc ccattcatca gttccatagg ttggaatcta aaatacacaa
      721 acaattagaa tcagtagttt aacacattat acacttaaaa attttatatt taccttagag
      781 ctttaaatct ctgtaggtag tttgtccaat tatgtcacac cacagaagta aggttccttc
      841 acaaagatcc aagcttacta cccaccgtac tcgtcaattc caagggcatc ggtaaacatc
      901 tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc gtggggggta
      961 aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc gtctagcgcg
     1021 tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc caggctgaca
     1081 tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa ggacaggcgc
     1141 ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag caggccctcg
     1201 atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggggcc cgagcccgat
     1261 tcacatttca attgtttttc caatccacaa ataatcaatt ccaagccgaa caagaatgct
     1321 ggttcggctc cttgatgatc gaacaattca attgcttgac gcaacaatgg gggcatcgaa
     1381 tctgttgttg gtgtttcacg ttcttcttta gcgacttgat gttcttgatc ttccaacaca
     1441 cagcccaatg taaaatgacc aacggcgctc aaagcataca aagcattttc caagctgaag
     1501 ccttgttgac acaagaaagc caattgattt tccaatgttt catattgttt ttctgttgga
     1561 cgtgtgccca aatgaacctt ggcaccatca cgatgggaca acaaagcaca acggaaggat
     1621 ttggcattat tacgcaagaa atcttgccag gattcgcctt ccaatgggca aaaatgtgta
     1681 tgatgacggt ccaacatttc aatagccaaa gcatccaaca aagcacgttt atttttaaca
     1741 tgccaataca atgttggttg ttcaacaccc aatttttgag ccaatttacg tgttgtcaaa
     1801 ccttcaatac caacttcatt caacaattcc aaagcggaat taataacttt ggatttatcc
     1861 aaacggctga ccatggctgc agatctggaa atacaatagg aaaaataaag ttagcgaatt
     1921 ggaaaacaat ttttcaaaca cattttatat tgttaaggga gaaaagggtg gtttttagat
     1981 acgttttgct ctacaaggtt ttttatatat accgttaggt tttaatataa cctttttaat
     2041 gctttttttt cattttcatg ataaaagatt ctaggttttc ttctttcgtt aaggggtggg
     2101 tttttgatct atgacgtcag acttaattag aattttcatt attataaaat ttttacaaat
     2161 tatttctttt tattaagaaa atataacttc ttttcggaat aagtggaagg aaaatggggg
     2221 tccaattttt tcccaaaatg ttagggttaa gaaaaaaaaa tcttaattct ttccggaatt
     2281 agtaaaaaag ggggtaccat tttgactcac ttttttaatg tcagggttac acaaaatagg
     2341 gttcgaatcg atttttttag aatttctagt ataatttgca acgttttata attttaaaaa
     2401 attcacttcc ctttttccca taacacgttg ttgcaaattt gtatgaaatt aactaatttt
     2461 caaaacttac cttgatgtgt tttattattc aattaatttc gcagaaaaaa taaattaatt
     2521 tgtagattct tttttttgca attgaaattg atcaaccaca atctaatata ttataacttt
     2581 tttcactttt cagttacaat aattaaatac tttttgtctt ttcgctcgct tggaaactct
     2641 cgatgtattt tgcacaattt ttctttttag actgatgccg gttcacacac atgcactgaa
     2701 tttatacaaa aaaacaaaaa taatggaaaa caccagaatg ttctagaaca aactactcgc
     2761 acttccgtag gctttaaaaa acatcatgtg gttgttattc attatgaaat taacaaaaaa
     2821 ctaaaaaatt tcaaatttat aaaaataatt ttaagaaaat cttttatata tttaataatc
     2881 gaatagatct tttgtcatga taattttaaa aaattaaaaa tgagaaaaaa atcaaagaaa
     2941 atttgccaca ttattaataa cccaacaagg ttgtattttt gcataagcaa taatttttaa
     3001 actgttttat tgcattaata cttgctcttt cttctctttt tgaatgtgag aaattaagtg
     3061 acatgtggta agaaaaatga atcaacaaaa ttaaacttta gctaaaattt attaaaatta
     3121 atgaataaat ataaaaataa attttgaaat gaagttaaat acttaaactt aataatgact
     3181 aatcatacat agaactttat agaaatcttg caaataaaca gaaaccattt tgagggagaa
     3241 tagtatattg aactttttac acaaatttga aataaattat taattgtatt attaataaca
     3301 attgtattcc tgccttatta atatgcctta aattgtaaaa attaattaat acaagtctaa
     3361 aggttgagca ttaaattttt aactgtaaaa tttaatcctt taaaaaatat tttaactaat
     3421 acgtctttat taaaggtcat gttagataat cagattcctt gttagttata aataatagtt
     3481 taatttatca taatcaatat ttgattatct attatgcata atttgttagc aaaaaaattg
     3541 tatttttaag aaaacaaaac cttacaaatg cattttgtgc aaatatttcg attctctttt
     3601 ctctcttttt taagcaattg tgggcattgt tgttaatttt ttgccggtgc agcagatgtc
     3661 tttttttcaa catggttgcc aacgttgtaa taaaaaaggt agaaaaaatt gagaacaaat
     3721 taagaatctt tctttctttt aatcatgaga gcataaaagt gaaatcgaaa cttttaacat
     3781 gtcagtgatt atgtataatg tcaatcagat taaccatata atctgattaa aaatgagtga
     3841 ccagaaaaaa gtgcatacta aaaaattaaa tatttttcca caaaatcaaa ctttattcgt
     3901 cacgaagtta acctagcatt caaagatttg ctgttaaact gtttataact ccattaaaca
     3961 gtaccggtct ttggtagaaa gaatggtccg aatgatgtct ctaaagccaa aaaatataca
     4021 gctctttcaa tacaactcct aatacctccg gaagtaaagc agtccggtta gctccgtgtt
     4081 aggactattt ggtacaaaaa gttaaaacta atgcaggtta agagcataca agcctcaaaa
     4141 gttctcgaca ggaaagctgc tgaaaattaa gaggcctaaa acagtgaacg gaaattgaag
     4201 acaaatttta ttaaaaaaaa tattttttgt atagtggatg acgaaacgca acttcctaat
     4261 aaaaaaaaat tgttgctgat gttccaggga attttaaaaa agtatatatt tactgaaaaa
     4321 ttcttggtat atcaaacaat atgaagttgc agcaaaagaa accatcaatt tgttacaaaa
     4381 tctgtatata aatactgaaa ttttcatcga gaaatgttta caaagagagc tgctttcatc
     4441 catcagactt catattatgt tcacttatat ctgaactgtc aagacatgct gtcattatgg
     4501 taaacaagcc cttaagtgat acaataacaa taatgtgata tttgtatcaa gagagtcaaa
     4561 tcattcctat tgccctaagc tacggtcagt ttagatatac tggaatcttg tgaaaataga
     4621 attagggagc acacaaaagg agtccaaaaa tgatgtagat tttaaaccga atgaattact
     4681 tgcttgagca aagtatcgga aagctccata aatacgttta tggatgggtt tccgaaaagt
     4741 tttgttataa aatttggtaa aattgagaaa aaataagaaa aatcgatatt ccatattttt
     4801 ttccattatt acattttgct aattcttggc gattttaatt tcccctttca ggcataatat
     4861 acaattgtta taacttaaac tctagtaatg acagcggccc acgtgttgaa accattatca
     4921 gaaaataatt cacttaaaca ttataatttg ataaaaagaa cactaaaaca aaatattata
     4981 attataccgc caaaaattca acaaacttta agaaatttcg agtaaattgc accgcaaact
     5041 aaaaaaccga catgataaac aaaaaaaata cataaaagag cgcaaacagc tgtcatttac
     5101 agggttgtag atatttgaaa tttttaccta aaaaggggaa aataaaaggc cacacttttg
     5161 gaaattacaa atttgtgaga gaatttttga tttcatttat taaatttata taaaaacaaa
     5221 agagttgtta taatatgtat gagataatgt ttgcaaattt taatttttaa atttttacag
     5281 tcaaatttta taaaatatgt taaaagtata tgattgtcac atttttaaat tattccactt
     5341 ttcattgttt ttattcaaga ccggcaactc tgatgttttg ctgcacatca gctgcactgg
     5401 cgaaaaaatt attaatgccc ccatgatgag agaaaatcaa tattttttca ttttatgcat
     5461 ttttataaat tttgttagca atattattgt tttaataatg ttattatttt ctttaataaa
     5521 acaattaaat tactttcatt tataatttat gatgactaat atgtaaatcg aatgtgatta
     5581 aatcatacat actatttcca tataaagcac aaagtttaat atataattaa tattaaattt
     5641 atttaattta aaaattaata gttaatacat atttcaatta atcaaataca tattaagctt
     5701 gtaacttagt tcaaaaaaca cttaattata catagatttg tcaattaata agacttttta
     5761 actataattt aaagaataaa acgtttttga aataaataag ttcattgttt gactagaaaa
     5821 atatttagtt tatgtcatga tattactcag caatgcctaa gattaattcc tttttcattt
     5881 aattaataac ttttatatat ttttgcataa ataaataaag taaattatag ttgattcatt
     5941 ccgcatgtta ttaaaaacag agagaacatt gtagaaattt tgacattgat gcaattttac
     6001 agtttaaaag ccactgcttc tacatatata caacgctgcg tgggcattaa tcatgttgaa
     6061 tgttttgcac atttttttga taatttgcaa ctaataaatt aaaataacaa taaaaaccta
     6121 taattaatat tggttttcta caattttaat aaattaattt ttttatatat ctaaaatgta
     6181 accaatttct tttgttatca ccaaataaac aaccacatga tgctgtttag agcctacgga
     6241 agtacgggta gtttgttcca gaacattcta gtattttcgt ttatttttgt tttacctata
     6301 aattcagtgc atggctgcta accggcatca gtcttgaaag aaaaattgtg caaaaaacac
     6361 caagaattcc aaacgggaga aagttaaaag tgttttaatt ttcttaataa agaaaagtct
     6421 gaaaaaagaa cattaaaaaa gtgtgtagtt gtttaatttc aattgcaaaa agaaaaataa
     6481 tttaaaaagt atttattttt gtgaaattaa tataaaacaa acacatcaag gcaagttttt
     6541 attaaaaaaa ataaaatgta gtaacaaaac gtttttgttc ttttgaaaaa agtgtgatgt
     6601 aaaaactcag gtaatttgcc agaaagaaaa accttcaata tggtttaaga ataacgttac
     6661 taagtaattg taaattgttc tagattaaat tttctttggc catgacattt ttagaaaata
     6721 aatctagtat ataaagaaaa tttcaccatg ctaataaaaa gaaatttcta gcatgcgatt
     6781 ttttaaagaa aatttttcaa aattttttcg aaattataat cgatatttca tgttttcatt
     6841 cgttaaaaag catacccaat ccacccctaa aaaactagta attcttaact taaagagatt
     6901 tctagatttt tattaaaaat tttcagtacc aatcccactc cctacactta acgaaaaacg
     6961 tgcgtcatta aaattattta tacttcatat tttgattgat tattttacta ataatttgta
     7021 tttttctatt ttttttttat taaacacaga tgcctgaaga agttgaaact tttgctttcc
     7081 aagctgaaat tgctcagctt aaaggatcca tggctcagtc aaagcacggt ctaacaaaag
     7141 aaatgacaat gaaataccgt atggaagggt gcgtcgatgg acataaattt gtgatcacgg
     7201 gagagggcat tggatatccg ttcaaaggga aacaggctat taatctgtgt gtggtcgaag
     7261 gtggaccatt gccatttgcc gaagacatat tgtcagctgc ctttatgtac ggaaacaggg
     7321 ttttcactga atatcctcaa gacatagctg actatttcaa gaactcgtgt cctgctggtt
     7381 atacatggga caggtctttt ctctttgagg atggagcagt ttgcatatgt aatgcagata
     7441 taacagtgag tgttgaagaa aactgcatgt atcatgagtc caaattttat ggagtgaatt
     7501 ttcctgctga tggacctgtg atgaaaaaga tgacagataa ctgggagcca tcctgcgaga
     7561 agatcatacc agtacctaag caggggatat tgaaagggga tgtctccatg tacctccttc
     7621 tgaaggatgg tgggcgttta cggtgccaat tcgacacagt ttacaaagca aagtctgtgc
     7681 caagaaagat gccggactgg cacttcatcc agcataagct cacccgtgaa gaccgcagcg
     7741 atgctaagaa tcagaaatgg catctgacag aacatgctat tgcatccgga tctgcattgc
     7801 cctgaaagct tgcagccaaa cccatcatcg acaaccatca aaaccaaaaa aaaaagacag
     7861 aagaagtgtg tattgaaaaa attctcgacc tattagctga aactactcaa ctaaacattc
     7921 aatatacttt tttttaaaca aactgttatt ttaaaaatga taacgaatcc aaaacaaaac
     7981 acttgtaaat tttttttatt ttcaaatttg aaaaaaacat gaatgaaaag aacaaatttt
     8041 aaagaaatgc actttaaaaa cacgaaaaaa aaacaagaac ctaataaaat gaaaggctta
     8101 agctgcattg aatcataata ttccaaaaga aaatttttta atgttttact acttaatggg
     8161 gggaaaaaga gggaaaacaa ctaaaaatag taattgttaa aatgcttggc aaaagtttaa
     8221 tgaacttgcc gaggtgtaga ggaaactata aaaatgcata gtgagtgcat taaaaacagc
     8281 actagtttgg cctcattttg atgggtacaa gaggaagacg aacaatttta aaaataactt
     8341 tcaagtggaa gatagatttg cttgaaaaca ttttgagaat ttactctagc tataaagata
     8401 aataacatta cttccktywt wcccaagatt tctttaaact atagatgact cattaatgaa
     8461 tcgttgcttt ttatttatga tttccctccc ctcttaaaca agtacagttt gttttctata
     8521 tacataccta ctcagagttc tattttttgt tagcaacagc tctgccaact gcttgttgcc
     8581 ttttaataga gttttgttgt tgctgtgttg gtcttaaaat catatgaagc attttatgtg
     8641 gtgggtttta taactatttt tgtttggata tagtattttt gttttctgta ctgtaactca
     8701 acaacacatt gccttttatg gtcatataaa tgagtaaata tactatattg tatctaccac
     8761 gatgatctca ttcacatttt ttagagttta cattggaaac aaatatttga attttgtaag
     8821 aaaacactgc ccaccaaatt tttaagttgt ttatttggat ctttgtgtaa aagaggggtt
     8881 ttaagataaa tttcaagttc attttaatgt atcatgggac gctaagatca aagaatattt
     8941 gttttaagta atgtaaagtt atattttctg cttgatcaaa tctattataa gatcttatat
     9001 cacacagtga attagttccc aagaaagggg taaagatgca tatcatttag cttaaacgtt
     9061 tttcataaaa ctgaacgtca tgggcaaatt ttcattaaag tataattttc tccaaagaac
     9121 tggtctattg tcctcactat tttatatttc catagtactg gacaggaacg cggtttaagg
     9181 atttgcgttt tagtttaaca taaatcaaag tttttgacta caccagcccg cactgacgga
     9241 cacaccgaag ccccggcggc aaccctcagc ggatgccccg gggcttcacg ttttcccagg
     9301 tcagaagcgg ttttcgggag tagtgcccca actggggtaa cctttgagtt ctctcagttg
     9361 ggggcgtagg gtcgccgaca tgacacaagg ggttgtgacc ggggtggaca cgtacgcggg
     9421 tgcttacgac cgtcagtcgc gcgagcgcga ctagtacaag ccgaattctg cagatatcca
     9481 tcacactggc ggccgcgtcg acccgcggcc atggaggcct ggtaccagat ctgacaatgt
     9541 tcagtgcaga gactcggcta cgcctcgtgg actttgaagt tgaccaacaa tgtttattct
     9601 tacctctaat agtcctctgt ggcaaggtca agattctgtt agaagccaat gaagaacctg
     9661 gttgttcaat aacattttgt tcgtctaata tttcactacc gcttgacgtt ggctgcactt
     9721 catgtacctc atctataaac gcttcttctg tatcgctctg gacgtcatct tcacttacgt
     9781 gatctgatat ttcactgtca gaatcctcac caacaagctc gtcatcgctt tgcagaagag
     9841 cagagaggat atgctcatcg tctaaagaac tacccatttt attatatatt agtcacgata
     9901 tctataacaa gaaaatatat atataataag ttatcacgta agtagaacat gaaataacaa
     9961 tataattatc gtatgagtta aatcttaaaa gtcacgtaaa agataatcat gcgtcatttt
    10021 gactcacgcg gtcgttatag ttcaaaatca gtgacactta ccgcattgac aagcacgcct
    10081 cacgggagct ccaagcggcg actgagatgt cctaaatgca cagcgacgga ttcgcgctat
    10141 ttagaaagag agagcaatat ttcaagaatg catgcgtcaa ttttacgcag actatctttc
    10201 tagggttaaa aaagatttgc gctttactcg acctaaactt taaacacgtc atagaatctt
    10261 cgtttgacaa aaaccacatt gtggccaagc tgtgtgacgc gacgcgcgct aaagaatggc
    10321 aaaccaagtc gcgcgagcgt cgactctaga ggatccccgg gtaccgagct cgaattcgta
    10381 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    10441 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    10501 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    10561 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    10621 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    10681 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10741 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10801 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10861 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10921 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10981 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    11041 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    11101 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    11161 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    11221 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    11281 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    11341 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    11401 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    11461 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    11521 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    11581 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    11641 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    11701 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11761 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11821 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11881 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11941 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    12001 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    12061 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    12121 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    12181 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    12241 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    12301 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    12361 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    12421 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    12481 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
    12541 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
    12601 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
    12661 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
    12721 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
    12781 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    12841 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
    12901 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
    12961 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tttgtttaaa
    13021 atataacaaa attgtgatcc cacaaaatga agtggggcaa aatcaaataa ttaatagtgt
    13081 ccgtaaactt gttggtcttc aactttttga ggaacacgtt ggacggcaaa tccgtgacta
    13141 taacacaagt tgatttaata attttagcca acacgtcggg ctgcgtgttt tttgccgacg
    13201 cgtctgtgta cacgttgatt aactggtcga ttaaactgtt gaaataattt aatttttggt
    13261 tcttctttaa atctgtgatg aaatttttta aaataacttt aaattcttca ttggtaaaaa
    13321 atgccacgtt ttgcaacttg tgagggtcta atatgaggtc aaactcagta ggagttttat
    13381 ccaaaaaaga aaacatgatt acgtctgtac acgaacgcgt attaacgcag agtgcaaagt
    13441 ataagagggt taaaaaatat attttacgca ccatatacgc atcgggttga tatcgttaat
    13501 atggatcaat ttgaacagtt gattaacgtg tctctgctca agtctttgat caaaacgcaa
    13561 atcgacgaaa atgtgtcgga caatatcaag tcgatgagcg aaaaactaaa aaggctagaa
    13621 tacgacaatc tcacagacag cgttgagata tacggtattc acgacagcag gctgaataat
    13681 aaaaaaatta gaaactatta tttaacccta gaaagataat catattgtga cgtacgttaa
    13741 agataatcat gcgtaaaatt gacgcatgtg ttttatcggt ctgtatatcg aggtttattt
    13801 attaatttga atagatatta agttttatta tatttacact tacatactaa taataaattc
    13861 aacaaacaat ttatttatgt ttatttattt attaaaaaaa aacaaaaact caaaatttct
    13921 tctataaagt aacaaaactt ttaaacattc tctcttttac aaaaataaac ttattttgta
    13981 ctttaaaaac agtcatgttg tattataaaa taagtaatta gcttaactta tacataatag
    14041 aaacaaatta tacttattag tcagtcagaa acaactttgg cacatatcaa tattatgctc
    14101 tcgacaaata acttttttgc attttttgca cgatgcattt gcctttcgcc ttattttaga
    14161 ggggcagtaa gtacagtaag tacgtttttt cattactggc tcttcagtac tgtcatctga
    14221 tgtaccaggc acttcatttg gcaaaatatt agagatatta tcgcgcaaat atctcttcaa
    14281 agtaggagct tctaaacgct tacgcataaa cgatgacgtc aggctcatgt aaaggtttct
    14341 cataaatttt ttgcgacttt ggaccttttc tcccttgcta ctgacattat ggctgtatat
    14401 aataaaagaa tttatgcagg caatgtttat cattccgtac aataatgcca taggccacct
    14461 attcgtcttc ctactgcagg gtaaccaccg gttctagac
//
