Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | CS111 | Antibiotic Resistance | Ampicillin |
| Length | 4146 bp | Type | Expression vector |
| Source | Khokha MK, Hsu D, Baker JC, Harland RM. | ||
CS111 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CS111 vector Sequence
LOCUS Exported 4146 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Expression vector CS111, complete sequence.
ACCESSION DQ649434
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4146)
AUTHORS Khokha MK, Hsu D, Baker JC, Harland RM.
TITLE Vectors for Expression Cloning in Xenopus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4146)
AUTHORS Khokha MK, Hsu D, Baker JC, Harland RM.
TITLE Direct Submission
JOURNAL Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA
94720-3200, USA
REFERENCE 3 (bases 1 to 4146)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..4146
/organism="Expression vector CS111"
/mol_type="other DNA"
/db_xref="taxon:388338"
promoter 35..53
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 35..51
/label=Sp6
/note="Sp6"
primer_bind complement(161..183)
/label=T7
/note="T7"
promoter complement(164..182)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 187..384
/label=SV40 polyA
/note="SV40 polyA"
polyA_signal 187..321
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(437..456)
/label=T3
/note="T3"
promoter complement(438..456)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
misc_feature join(467..696,3086..3155)
/label=lac operon
/note="lac operon"
primer_bind complement(477..493)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 501..517
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(525..555)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 570..591
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin 823..1490
/label=ColE1
/note="ColE1"
rep_origin complement(879..1467)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1638..2498)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=bla
/note="ampicillin resistance"
/protein_id="ABF93445.1"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
gene complement(1638..2498)
/gene="bla"
/label=bla
promoter complement(2499..2603)
/gene="bla"
/label=AmpR promoter
misc_feature 2628..3084
/label=PhageM13
/note="PhageM13"
rep_origin complement(2629..3084)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
regulatory 3162..4146
/regulatory_class="promoter"
/note="CMV-IE94"
promoter 3162..4146
/label=CMV IE94 promoter
/note="enhancer/promoter region of simian cytomegalovirus
major immediate early transcription unit IE94"
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