CS111 vector (V010029#)

Basic Vector Information

Basic Vector Information
Vector Name CS111 Antibiotic Resistance Ampicillin
Length 4146 bp Type Expression vector
Source Khokha MK, Hsu D, Baker JC, Harland RM.

CS111 vector Vector Map

CS1114146 bp60012001800240030003600SP6 promoterT7SV40 polyAT3lac operonColE1blaAmpR promoterPhageM13CMV-IE94

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CS111 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                4146 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector CS111, complete sequence.
ACCESSION   DQ649434
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Vectors for Expression Cloning in Xenopus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Expression vector CS111"
                     /mol_type="other DNA"
                     /db_xref="taxon:388338"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..51
                     /label=Sp6
                     /note="Sp6"
     primer_bind     complement(161..183)
                     /label=T7
                     /note="T7"
     promoter        complement(164..182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    187..384
                     /label=SV40 polyA
                     /note="SV40 polyA"
     polyA_signal    187..321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(437..456)
                     /label=T3
                     /note="T3"
     promoter        complement(438..456)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    join(467..696,3086..3155)
                     /label=lac operon
                     /note="lac operon"
     primer_bind     complement(477..493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    501..517
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(525..555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    570..591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      823..1490
                     /label=ColE1
                     /note="ColE1"
     rep_origin      complement(879..1467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1638..2498)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=bla
                     /note="ampicillin resistance"
                     /protein_id="ABF93445.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(1638..2498)
                     /gene="bla"
                     /label=bla
     promoter        complement(2499..2603)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    2628..3084
                     /label=PhageM13
                     /note="PhageM13"
     rep_origin      complement(2629..3084)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     regulatory      3162..4146
                     /regulatory_class="promoter"
                     /note="CMV-IE94"
     promoter        3162..4146
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"

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