LOCUS       40924_550        4146 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Expression vector CS111, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Vectors for Expression Cloning in Xenopus
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Khokha MK, Hsu D, Baker JC, Harland RM.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 
            94720-3200, USA
REFERENCE   3  (bases 1 to 4146)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4146)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (25-MAY-2006) MCB, UC-Berkeley, 142 LSA, Berkeley, CA 94720-3200, 
            USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        complement(164..182)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    complement(187..321)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(438..456)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(477..493)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(501..517)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(525..555)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(570..591)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(879..1467)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1641..2498)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2499..2603)
                     /label=AmpR promoter
     rep_origin      complement(2629..3084)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3162..4146
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga ttcgaattcg tcccgggaat ctggccgcgg
      121 ccgcaaggcc tgatatctct agaccgcggc tcgagcctct cgccctatag tgagtcgtat
      181 tacgtagatc cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
      241 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
      301 ataagctgca ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag
      361 ggggaggtgt gggaggtttt ttaattcgcg gcgcgccgcg gcgccaatgc attgggcccg
      421 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
      481 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
      541 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
      601 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
      661 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
      721 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      781 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
      841 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
      901 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
      961 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1021 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1081 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1141 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1201 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1261 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1321 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1381 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1441 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1501 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1561 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1621 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1681 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1741 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1801 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     1861 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     1921 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     1981 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2041 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2101 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2161 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2221 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2281 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2341 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2401 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2461 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2521 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2581 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa attgtaagcg
     2641 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     2701 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     2761 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     2821 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     2881 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     2941 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     3001 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     3061 ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac tgttgggaag
     3121 ggcgatcggt gcgggcctct tcgctattac gccagtcgat cgaccatagc caattcaata
     3181 tggcgtatat ggactcatgc caattcaata tggtggatct ggacctgtgc caattcaata
     3241 tggcgtatat ggactcgtgc caattcaata tggtggatct ggaccccagc caattcaata
     3301 tggcggactt ggcaccatgc caattcaata tggcggactt ggcactgtgc caactgggga
     3361 ggggtctact tggcacggtg ccaagtttga ggaggggtct tggccctgtg ccaagtccgc
     3421 catattgaat tggcatggtg ccaataatgg cggccatatt ggctatatgc caggatcaat
     3481 atataggcaa tatccaatat ggccctatgc caatatggct attggccagg ttcaatacta
     3541 tgtattggcc ctatgccata tagtattcca tatatgggtt ttcctattga cgtagatagc
     3601 ccctcccaat gggcggtccc atataccata tatggggctt cctaataccg cccatagcca
     3661 ctcccccatt gacgtcaatg gtctctatat atggtctttc ctattgacgt catatgggcg
     3721 gtcctattga cgtatatggc gcctccccca ttgacgtcaa ttacggtaaa tggcccgcct
     3781 ggctcaatgc ccattgacgt caataggacc acccaccatt gacgtcaatg ggatggctca
     3841 ttgcccattc atatccgttc tcacgccccc tattgacgtc aatgacggta aatggcccac
     3901 ttggcagtac atcaatatct attaatagta acttggcaag tacattacta ttggaagtac
     3961 gccagggtac attggcagta ctcccattga cgtcaatggc ggtaaatggc ccgcgatggc
     4021 tgccaagtac atccccattg acgtcaatgg ggaggggcaa tgacgcaaat gggcgttcca
     4081 ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct atataagcaa tgctcgttta
     4141 gggaac
//
