Basic Vector Information
| Basic Vector Information | |||
|---|---|---|---|
| Vector Name | CIp30 | Antibiotic Resistance | Ampicillin |
| Length | 8492 bp | Type | Candida integrating vector |
| Source | Dennison PM, Ramsdale M, Manson CL, Brown AJ. | ||
CIp30 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
CIp30 vector Sequence
LOCUS Exported 8492 bp ds-DNA circular SYN 17-DEC-2018
DEFINITION Candida integrating vector CIp30, complete sequence.
ACCESSION DQ015895
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8492)
AUTHORS Dennison PM, Ramsdale M, Manson CL, Brown AJ.
TITLE Gene disruption in Candida albicans using a synthetic,
codon-optimised Cre-loxP system
JOURNAL Fungal Genet. Biol. 42 (9), 737-748 (2005)
PUBMED 16043373
REFERENCE 2 (bases 1 to 8492)
AUTHORS Dennison PMJ.
TITLE Direct Submission
JOURNAL Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS,
Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE 3 (bases 1 to 8492)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..8492
/organism="Candida integrating vector CIp30"
/mol_type="other DNA"
/label=derived from Candida albicans
/note="derived from Candida albicans"
/db_xref="taxon:329971"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 626..644
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 670..2060
/label=URA3 marker
/note="URA3 marker"
misc_feature 2072..4076
/label=ARG4 marker
/note="ARG4 marker"
misc_feature 4083..5366
/label=HIS1 marker
/note="HIS1 marker"
misc_feature 5372..6232
/label=RPS1
/note="RPS1"
primer_bind complement(6258..6274)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
promoter complement(6304..6322)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(6343..6359)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 6367..6383
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6391..6421)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6436..6457
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(6745..7333)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7504..8364)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8365..8469)
/gene="bla"
/label=AmpR promoter
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