CIp30 vector (V010037#)

Basic Vector Information

Basic Vector Information
Vector Name CIp30 Antibiotic Resistance Ampicillin
Length 8492 bp Type Candida integrating vector
Source Dennison PM, Ramsdale M, Manson CL, Brown AJ.

CIp30 vector Vector Map

CIp308492 bp4008001200160020002400280032003600400044004800520056006000640068007200760080008400f1 oriM13 fwdT7 promoterURA3 markerARG4 markerHIS1 markerRPS1KS primerT3 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

CIp30 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                8492 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Candida integrating vector CIp30, complete sequence.
ACCESSION   DQ015895
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8492)
  AUTHORS   Dennison PM, Ramsdale M, Manson CL, Brown AJ.
  TITLE     Gene disruption in Candida albicans using a synthetic, 
            codon-optimised Cre-loxP system
  JOURNAL   Fungal Genet. Biol. 42 (9), 737-748 (2005)
  PUBMED    16043373
REFERENCE   2  (bases 1 to 8492)
  AUTHORS   Dennison PMJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-APR-2005) Medical Sciences, Aberdeen University, IMS, 
            Foresterhill, Aberdeen AB25 2ZD, UK
REFERENCE   3  (bases 1 to 8492)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8492
                     /organism="Candida integrating vector CIp30"
                     /mol_type="other DNA"
                     /label=derived from Candida albicans
                     /note="derived from Candida albicans"
                     /db_xref="taxon:329971"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    670..2060
                     /label=URA3 marker
                     /note="URA3 marker"
     misc_feature    2072..4076
                     /label=ARG4 marker
                     /note="ARG4 marker"
     misc_feature    4083..5366
                     /label=HIS1 marker
                     /note="HIS1 marker"
     misc_feature    5372..6232
                     /label=RPS1
                     /note="RPS1"
     primer_bind     complement(6258..6274)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(6304..6322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6343..6359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6367..6383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6391..6421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6436..6457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6745..7333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7504..8364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8365..8469)
                     /gene="bla"
                     /label=AmpR promoter

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